ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb01445.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

The synthetic pentapeptide pGlu‐Glu‐Asp‐Cys‐Lys has recently been proposed as the active component of a granulocyte‐derived inhibitor of normal haematopoiesis. We investigated its biological activity on leukaemic myelopoiesis both in vitro and in vivo in rats. Three different human permanent myeloid leukaemic cell lines (HL60, KG1, ML3) and a rat transplantable acute myeloid leukaemia (Shay leukaemia) were studied. Neither HL60 nor KG1 were sensitive to the peptide whereas a consistently reproducible inhibition of3H‐TdR uptake was observed in ML3 cells. This effect was not due to a unspecific toxic action on target cells and was spontaneously reversible. When injected i.p. twice daily at an appropriate concentration in rats bearing Shay leukaemia, the peptide caused a significant increase in survival. Our results therefore indicate that the synthetic pentapeptide studied inhibits not only normal but also leukaemic m

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb01446.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

We studied the chromosomes in the bone marrow of 4 patients who had both diabetes insipidus (DI) and acute non‐lymphocytic leukaemia. Clinical findings suggested that, in each case, myelodysplastic syndrome had preceded the onset of acute leukaemia. Two other such patients described in the literature had had a banded karyotype study of bone marrow cells. All 6 patients had deletions of chromosome 7. 3 had monosomy 7 as the sole cytogenetic abnormality, 2 had monosomy 7 associated with other clonal abnormalities and 1 had del(7)(q22) in association with other abnormalities. These data suggest that monosomy 7 or perhaps monosomy for 7q22‐qter predisposes to DI. The mechanism by which the proposed predisposition is produced remains to be clarif

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb01447.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

In 7 cases of chronic B‐cell lymphoproliferative disorders – 6 chronic lymphocytic leukaemias and 1 non‐Hodgkin lymphoma in leukaemic phase – which co‐expressed T‐cell markers (CD3, CD2) the clonal origin was investigated at the DNA level. In accordance with the diagnosis, all cases showed a monoclonally rearranged configuration of the immunoglobulin genes. On the contrary, the T‐cell receptor beta chain gene always retained a germ‐line organization. These findings demonstrate that B‐cell chronic lymphoproliferative disorders which co‐express T‐cell‐related markers are truly composed of mon

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb01448.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

The effect of human recombinant interferon α2(IFNα2) on hairy cells obtained from 16 patients was evaluated. All patients promptly responded to induction of remission with 2 times 106U/m2interferon α2b, three times a week, sc. In order to achieve a more detailed insight into the mode of action of interferon in this disease, we determined the influence of IFNα2on the incorporation of radiolabeled thymidine and uridine into hairy cells. While both3H‐thymidine and3H‐uridine incorporation were unaffected by IFNα2in a 3‐hour incubation period, a significant increase in uridine incorporation into hairy cells, but not CLL cells, was observed after 24 h. Cell surface marker analysis performed with monoclonal antibodies did not reveal a quantitative alteration of the immunophenotype of hairy cells in vitro. In addition, natural killer cells, assessed by monoclonal antibodies and a cytotoxicity assay against K 562 cells, were found to be decreased in 9 out of 10 patients prior to therapy. Although IFNα2could stimulate natural killer cells in vivo, we did not find a consistent correlation between the activation of these cells and the response to therapy. We conclude, therefore, that NK cells play no major role in the regression of hairy cells. Furthermore, IFNα2does not alter antigenic determinants in vitro, but leads to an enhanced incorporation of3H‐uridine into hairy cells in vitro, thus indicating a possible role for the induction of RNA syn

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb01449.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

We studied the pharmacokinetic data of 13 subjects with hemophilia B treated with a single‐dose of a Factor IX concentrate (Bebulin TIM2, N = 9; Preconativ, N = 4). The decay curves of Factor IX were evaluated by model‐independent methods and the following pharmacokinetic parameters (mean ± SD) were estimated: clearance (ml/h/kg) = 4.99 ± 2.01; mean residence time (h) = 22.9 ± 10.6; volume of distribution (ml/kg) = 99.9 ± 35.5. The in vivo recovery (59.8% ± 16.9%) was found to be inversely correlated with the volume of distribution. No significant difference in the pharmacokinetic parameters was found between patients treated with Preconativ and those treated with Bebulin. A model‐dependent compartmental evaluation of the 13 decay curves showed that the two‐compartment model was better than the one‐compartment model in 7 cases (53.8%), but the improvement of fit resulting from the two‐compartment model was statistically significant in only

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb01450.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb01451.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Spleen function was evaluated by measurement of the clearance of autologous heat‐damaged99mTc‐labelled erythrocytes from the circulation and into the spleen and the enumeration of pitted erythrocytes by interference contrast microscopy, and the spleen area was determined by scintillation scanning. All measurements were performed on 12 patients with chronic myelogenous leukemia and compared with 10 controls with apparently normal spleens, 6 splenectomized subjects and 9 patients with a reactive splenomegaly. Patients with CML had spleen function test results similar to normal controls in spite of having enlarged spleens whose projection area did not differ from that of the patients with reactive splenomegaly. Thus, patients with CML have a decreased spleen function per unit volume and signs of splenic hypofunction in the peripheral blood. The reduction of spleen function per unit volume in CML is the result of a severe decrease of the splenic blood perfusion which could result from the combined effects of the myeloid metaplasia and the increased whole‐blood viscosity due to high white‐cell

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb01452.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

As morbidity of thrombopenia in hemophilia A patients is increasing, the pathogenetic influence of the reticuloendothelial system (RES) was measured using autologous anti‐Rh0(D)‐coated erythrocytes (EA) in 17 patients with or without thrombopenia. Mean survival of EA in patients was reduced to 53% of healthy controls (53.2 ± 46.1 min vs 100.5 ± 12.2 min; patients vs controls, mean ± S.D.). Survival of EA was not significantly different either in thrombopenic vs non‐thrombopenic nor anti‐HIV (human immunodeficiency virus) positive vs negative patients. Thrombopenia, elevated serum IgG and circulating immune complexes were related to the presence of anti‐HIV antibodies. EA survival was also decreased in the absence of anti‐HIV antibodies. This indicates activation of RES by a mechanism different from retroviral infect

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb01453.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

The total urinary hydroxyproline excretion was assessed in 47 patients with chronic myeloproliferative disorders. Urinary hydroxyproline excretion was normal in 16 patients with idiopathic myelofibrosis and in 5 out of 6 patients with acute myelofibrosis. In patients with osteomyelosclerosis (n = 8) values for urinary hydroxyproline excretion were higher (median 202, range 54–652) than those in idopathic myelofibrosis (median 139, range 84–216). This difference was not significant (p>0.1). Elevated values for urinary hydroxyproline excretion were found in 10 patients (1 AMF patient, 3 OMS patients and 6 patients with CML in the accelerated phase of the disease). All but 1 of these patients had been treated, or were being treated, with cytotoxic agents at the time of investigation. These findings are compatible with impaired degradation of bone marrow collagen which, together with enhanced collagen synthesis from bone marrow fibroblasts, accounts for progressive accumulation of connective tissue in the bone marrow. This process appears to be influenced by cytotoxic treatment as reflected in increased urinary hydroxyproline excretion in those patients receiving cytotoxic age

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb01454.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY