ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00300.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

In in vitro experiments, tumor necrosis factor (rHuTNFα) was found to inhibit spontaneous and directed migration of normal human neutrophils and monocytes. RHuTNFα showed no chemoattractant activity. In conjunction with a Phase‐2 clinical trial, we also studied in vivo rHuTNFα effects on neutrophil and monocyte number and function. At the time of maximal plasma TNF levels (30 min), marked neutropenia and monocytopenia were observed. Isolated neutrophils were activated for superoxide production but were unable to migrate. Monocyte migration was inhibited at later times. Neutrophil migratory function recovered between 4 and 8 h but was again depressed at 24 h. The data demonstrate the complexity of the response to TNF, comprising direct and indirect effects which are concentration‐, time‐ and place‐dependent. They further suggest that the only neutrophils and monocytes available for participation in an anti‐tumor activity of TNF in vivo are those present in the tumor a

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00301.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Alpha‐interferon (IFN) and 2‘‐deoxycoformycin (dCF) both exhibit substantial activity in the treatment of hairy cell leukemia (HCL). Anecdotal reports have suggested that patients who failed IFN could achieve durable responses with dCF, although the frequency with which this was said to have occurred was unknown. We reviewed the available data on the responsiveness of HCL to dCF after IFN therapy by analyzing cases reported in the literature and those treated under the Special Exception mechanism of the National Cancer Institute, Division of Cancer Treatment. Of 60 such cases identified there were 22 (37%) “compete responses” and 22 (37%) “partial responses” for a total response rate of 74%. Responses appeared to be durable in many cases, lasting up to 2 years at the time of reporting. dCF is an active agent in HCL both as initial therapy and for the salvage of patints who have failed IFN. The relative activity of these two agents and the optimal strategies for their use are currently under investigation in ongoing cl

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00302.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

We investigated the possibility that T cells observed in lymph nodes involved by B‐non‐Hodgkin's lymphomas (B‐NHL) may have a direct role in the expression of Mu‐ or Gamma‐ heavy chain isotype by autologous malignant B cells. T cells were separated from lymph nodes involved by B‐NHL cells expressing either surface IgM (19 cases) or surface IgG (4 cases) and compared to peripheral blood T lymphocytes of healthy subjects (19 cases) for their ability to promote both IgG and IgM secretion in Cowan‐activated normal B lymphocytes. The mean values of IgG/IgM ratios obtained under the influence of T cells associated with malignant B cells expressing either surface IgM or surface IgG were not statistically different to that obtained with the help of control T cells (0.60 and 0.62 versus 0.47, respectively). These results do not account for the hypothesis that autologous lymph node T cells may directly affect the expression of the heavy chain isotype by malignan

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00303.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

13 patients with refractory or relapsed acute lymphoblastic leukemia (ALL) and 7 patients with acute myeloid leukemia (AML) were treated with a regimen that included idarubicin 12 mg/m2intravenously daily for 3 d plus cytarabine 2 g/m2by infusion over 3 hours daily for 3 d. There were 10 remissions (ALL:7; AML:3) in the 15 relapsed patients and 4 (ALL:3) in the 5 patients with primary refractory disease. Severe myelosuppression was observed in all patients. Toxicity of this regimen caused nausea and vomiting, stomatitis, infections and/or liver enzymes increase. Cardiac toxicity was not observed. 2 patients died in aplasia of Gram‐negative septicemia and brain hemorrhage. In conclusion, the combination of idarubicin and intermediate‐dose cytarabine (IDARA‐C) seems to be highly effective and sufficiently well‐tolerated for the treatment of refractory and relapsed acute le

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00304.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

The effect of two conditioning regimens given prior to allogeneic bone marrow transplantation (BMT) on the kinetics of engraftment were compared. 5 patients received busulfan and cyclophosphamide: 7 patients received daunorubicin, vincristine, cytosine arabinoside, methylprednisone and VM‐26 plus total body irradiation (TBI). Bone marrow progenitors (BFU‐E, CFU‐E, CFU‐GM, CFU‐F) were assayed up to 3 months post‐BMT. All progenitors were severely depressed in spite of peripheral blood recovery. There was no stromal recovery in any adult patient post‐BMT. There was no significant difference in time to engraftment, or colony forming units, or between patients conditioned with chemotherapy alone or chemotherapy plus TBI. We were unable to detect effects of graft‐verus‐host disease or cytomegalic viral infection on bone marrow progenitors or peripheral blood recov

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00305.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

As part of a continuing study of the low MW iron pool, guinea pig reticulocytes were incubated with59Fe‐labeled transferrin, and the reticulocyte hemolysate was chromatographed on Sephadex G‐25.59Fe, in amounts corresponding to that which was in a low MW peak eluting from an Ultrogel column and to that not precipitated by ammonium sulfate, adsorbed to the Sephadex column. The adsorbing59Fe, on elution from the Sephadex with dilute formic acid, coeluted with phosphate and pentose. When EDTA was added to disrupt the putative iron complex, neither iron nor P adsorbed to the column, supporting the argument that they exist as a compound in the cytosol and adsorb and elute together for that reason. These observations provide additional evidence that P‐containing compounds, probably originating as nucleotides, are important components of the low MW iron pool of

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00306.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Patients aged 70 yr or older with multiple myeloma were treated, when suitable, according to concurrent trial protocols for younger patients, with the exception that the cytostatic regimen was not allocated at random. Intermittent melphalan and prednisone (MP) was given as the primary treatment to 42 patients and 5‐drug combination MOCCA to 68 patients. The groups were comparable with each other, and the distribution of the clinical stages of the patients was similar to the younger patients in concurrent trials. An at least 50% response was achieved in 33% (SE 7.3) with MP and in 75% (SE 5.3) with MOCCA. The median survival times were 39 and 32 months, the relative age‐adjusted survival times 45 and 41 months, respectively. Advanced age as such is thus no contraindication for active treatment of myeloma, and in suitable patients the results compare well with those achieved in younger patie

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00307.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Low‐grade non‐Hodgkin lymphomas (NHL) constitute a group of tumours with an often long survival time but, at present, with little — or no — chance of cure if the disease is not strictly local. In primarily asymptomatic patients, treatment may either be started immediately after diagnosis or deferred until symptoms occur. The possibility of predicting the symptom‐free time was investigated in 64 non‐selected initially asymptomatic patients with advanced low grade NHL, all of whom had treatment deferred until symptoms occurred. The most powerful predictor was the histopathological subgroup. Lymphocytic (LC) and follicular centroblastic‐centrocytic (fCBCC) lymphomas had a median symptom‐free period of 2 years, which was four times longer than that for immunocytoma (IC) and follicular and diffuse CBCC (fdCBCC). In addition, the serum levels of deoxythymidine kinase (S‐TK) and lactic dehydrogenase (S‐LDH) could predict the symptom‐free period. This did not apply to S‐Haptoglobin, S‐Orosomucoid or stage. In a multivariate analysis, only S‐TK gave additional information to histopathology. The only variable that predicted the overall survival time was the length

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00308.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

A hairy cell leukaemia (HCL) patient is presented in whom the peripheral blood mononuclear cells (PBMCs) carried suppressor T‐cell markers (CD3 +, CD2 +, CD8 + /CD4‐, CD38 +). Analysis of genomic DNA of PBMNC showed the presence of a monoclonal population of T cells, the T‐cell receptor (TCR) beta‐chain gene being rearranged on both alleles (DR/DR), while the immunoglobulin (Ig) heavy chain‐genes were in germline configuration. The neoplastic cells were found to react with the monoclonal antibody RAB‐1 — originally described as belonging to the B lineage‐restricted monoclonal antibodies — and to carry RAB‐1/CD‐8 in a double marker assay. Natural killer activity of PBMNCs against K562 target cells was severely reduced, while the cells were found to exert strong antibody‐dependen

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00309.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY