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1. |
THE HETEROCYSTS OF BLUE‐GREEN ALGAE (MYXOPHYCEAE) |
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Biological Reviews,
Volume 50,
Issue 3,
1975,
Page 247-284
V. V. S. TYAGI,
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摘要:
Summary1. Heterocysts are found in many species of filamentous blue‐green algae. They are cells of slightly larger size and with a more thickened wall than the vegetative cells.2. Structural details of the heterocyst are: the presence of three additional wall layers, the absence of granules, sparse thylakoid network throughout, except at the poles where a dense coiling of membranes occurs. Other characters include the two pores at opposite poles ‘plugged’ with refractive material called the polar granule.3. Peculiarities in the pigment composition of the heterocyst include an abundance of carotenoids and absence of phycobilins, and a short‐wave form of chlorophylla.4. Unique glycolipids and an acyl lipid, not found in the vegetative cells of the algae or in other plant cells, are associated with the heterocyst. The glycolipids constitute the laminated layer of the wall and probably regulate diffusion of substances through it, whereas the acyl lipids are supposed to function as carriers and intermediates in the biosynthesis of the wall.5. The heterocysts develop from vegetative cells, and the visible changes during differentiation include cell enlargement, synthesis of additional wall layers, disappearance of granules and reorientation and synthesis of the thylakoids.6. Heterocysts are formed sequentially with characteristic cellular spacing during the growth of cultures in medium free from combined nitrogen.7. Various sources of combined nitrogen inhibit heterocyst formation when supplied in the culture medium. Ammonium salts are among the most powerful inhibitors. Heterocysts are formed simultaneously and within a short period after transference of ammonia‐grown non‐heterocystous filaments to ammonia‐free medium.8. Incompletely differentiated heterocysts or proheterocysts are found in cultures grown in the presence of combined nitrogen. If two or more proheterocysts are close together generally a single one develops to maturity after a competitive interaction in medium free from combined nitrogen. This indicates that heterocyst formation is completed in two phases: phase I, synthesis and conservation of macromolecules, which takes place during growth in ammonia‐containing medium: and phase 11, morphological differentiation of the heterocyst which is unaccompanied by growth in cell number. In the ammonia‐free medium phase 11 quickly succeeds phase 1 and the whole process appears as a continuum.9. Heterocyst formation shows a definite requirement for light. Red light favours heterocyst formation, whereas green and blue light do not. The effects of light seem to be mainly due to photosynthesis, although some effects may be morphogenetic.10. Studies with metabolic inhibitors have revealed the involvement of photosynthesis, respiration and protein synthesis in heterocyst formation. Photosynthesis provides carbon skeletons, whereas ATP is most probably supplied by oxidative metabolism.11. Various functions have been assigned to the heterocyst from time to time. Their role in akinete formation is suggested by (i) the formation of akinetes adjacent to the heterocysts and (ii) prevention of sporulation by detachment of the heterocysts from the vegetative cells (potential akinetes). Despite substantial evidence for such a role, it is not applicable to all akinete‐forming genera.12. Heterocysts are now widely believed to be the site of nitrogen fixation in blue‐green algae. The main facts in favour of such a role are: (i) fixation of nitrogen by all heterocystous algae, (ii) inhibition of heterocyst formation by combined nitrogen and (iii) direct observations on acetylene reduction by isolated heterocysts.13. Some non‐heterocystous and unicellular algae, and vegetative cells of heterocystous algae fix nitrogen under microaerophilic conditions suggesting that absence of oxygen favours nitrogenase activity. Heterocysts lack the oxygen‐evolving photo‐system 11, possess oxidative enzymes, and reduce externally supplied tetrazolium salts ‐ all indicating that they are the most suitable sites for harbouring nitrogenase in aerobic conditions.14. Heterocysts probably originated in the Precambrian in response to the earth's changing environment and seem to be the first example of morphological differe
ISSN:1464-7931
DOI:10.1111/j.1469-185X.1975.tb00830.x
出版商:Blackwell Publishing Ltd
年代:1975
数据来源: WILEY
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2. |
THE ACTIVATION OF INFECTIVE STAGES OF ENDOPARASITES OF VERTEBRATES |
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Biological Reviews,
Volume 50,
Issue 3,
1975,
Page 285-323
ANN M. LACKIE,
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ISSN:1464-7931
DOI:10.1111/j.1469-185X.1975.tb00831.x
出版商:Blackwell Publishing Ltd
年代:1975
数据来源: WILEY
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3. |
SIALIC ACID AND THE SOCIAL BEEIAVIOUR OF CELLS |
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Biological Reviews,
Volume 50,
Issue 3,
1975,
Page 325-349
CLIVE W. LLOYD,
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摘要:
Summary1. It is suggested that specific carbohydrate side‐chains of membrane glycoproteins are the sites for cell recognition or adhesion when the terminal sugar, sialic acid, is absent.2. It is suggested that sialic acid plays a ‘protective’ or ‘blocking’ role in cell interactions so that addition of sialic acid to asialo side‐chains converts them to forms inactive for recognition. This principle of ‘blocking’ by sialic acid has been observed in other situations as in covering tumour antigens and in protecting glycoproteins from uptake by the liver. It is here extended to cell‐cell adhesions.3. It is to be expected that specific ‘protective’ actions of sialic acid in membrane‐bound glycoproteins will be difficult to detect. As a charged residue, sialic acid is likely to have a strong influence both on the glycoproteins on which it is borne and on their interactions with each other at the cell surface. Removal of sialic acid by enzymes could therefore perturb the structure of the cell surface in several ways and so obscure the ‘protective’ effects of sialic acid. Sialic acid is therefore suggested to have a structural role also.4. Evidence is assembled in favour of a model in which sialysation of specific adhesive receptors affects the social behaviour of cells. This may be an effect associated with growing cells since the contact properties of mitotic cells (and populations rich in dividing cells) are decreased by the increased sialysation of receptors. One of the factors associated with malignant behaviour could be that adhesive receptors are permanently blocked by sialic acid.5. A schematic representation of some of th
ISSN:1464-7931
DOI:10.1111/j.1469-185X.1975.tb00832.x
出版商:Blackwell Publishing Ltd
年代:1975
数据来源: WILEY
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4. |
IX. ADDENDUM |
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Biological Reviews,
Volume 50,
Issue 3,
1975,
Page 349-350
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ISSN:1464-7931
DOI:10.1111/j.1469-185X.1975.tb00833.x
出版商:Blackwell Publishing Ltd
年代:1975
数据来源: WILEY
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5. |
ACID‐BASE RELATIONSHIPS WITHIN THE AVIAN EGG |
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Biological Reviews,
Volume 50,
Issue 3,
1975,
Page 351-371
C. M. DAWES,
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摘要:
Summary1. In the newly laid egg of the domestic fowl the pH values of the albumen and yolk are about 7.6 and 6.0 respectively.2. When the egg is stored in air there is a loss of carbon dioxide from the albumen and the pH of this fluid rises to a maximum value of about 9.5. A large proportion of the carbon dioxide which remains in the albumen is in the form of carbonate.3. In the fertile incubated egg the pH of the albumen attains a maximum value within a period of about 2 days; the albumen then becomes less alkaline and it is nearly neutral by the end of the second week. The increasing acidity of the albumen can be attributed to (a) the secretion of hydrogen ions by the blastoderm and (b) the output of carbon dioxide by developing tissues.4. During the first 2 weeks of incubation the pH of the yolk progressively increases to a maximum value of about 7.5: there is then a tendency for the pH of this fluid to fall and the yolk that is retained within the body of the hatched chick is slightly acidic.5. The embryo may never come into direct contact with either the albumen or the yolk when the pH of these fluids are high and low respectively. At the beginning of embryonic development the blastoderm is separated from the albumen by the vitelline membrane and from the yolk by a layer of subgerminal fluid with a maximum pH of about 7.8. The vitelline membrane ruptures on day 4 but by this time the embryo is bathed in amniotic fluid with a pH of about 7.5.6. The pH of amniotic fluid falls from a maximum value of about 7.5 during week I to a minimum value of about 6.5 during week 2. Amniotic fluid is a simple solution of salts until day 12; albumen then begins to flow into the amniotic cavity and the buffering capacity of amniotic fluid increases.7. The principal end‐product of nitrogenous metabolism in the chick embryo is uric acid and about 100 mg of this substance are deposited within the allantoic cavity. The pH of allantoic fluid may exceed 7.5 during week 1 but falls to 6.0 or below after day 13.8. The tension of carbon dioxide within the egg is determined by the ratio of the rate of carbon dioxide production by the embryo to the permeability of the shell towards carbon dioxide. For the greater part of the period of incubation the permeability of the shell towards carbon dioxide is constant. Thus, as the carbon dioxide output of the embryo increases, the carbon dioxide tension within the egg rises.9. The pH of the blood can be defined in terms of the ratio of the bicarbonate concentration to the carbon dioxide tension. There is a progressive increase in the carbon dioxide tension of the blood during the period of incubation but the pH is maintained at about 7.4 by an increase in bicarbonate concentration.10. Part of the increase in bicarbonate is due to the removal of hydrogen ions from carbonic acid by haemoglobin. There is also a large influx of bicarbonate into the blood, but the source of this bicarbonate is not known; the evidence that renal mechanisms are involved is inconclusive and it is probable that the embryo utilizes the enormous potential store of bicarbonate in the egg shel
ISSN:1464-7931
DOI:10.1111/j.1469-185X.1975.tb00834.x
出版商:Blackwell Publishing Ltd
年代:1975
数据来源: WILEY
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