摘要:

AbstractTo clarify the factors producing bile infarction and bile duct proliferation in obstructive jaundice, the incidence of the hepatic lesions and the serum levels of the bile constituents were examined in three rat models. (1) Ligation of the common bile duct induced bile infarction, bile duct proliferation, retention of bite in the liver, and elevation of the serum levels of total bilirubin and total bile acids. (2) The rats treated by choledochotomy had bile in the abdominal cavity, but there was no retention of bile in the liver. The degree of development of bile infarction was similar to that of the common bile duct ligation group, but bile duct proliferation was not found: the serum levels of total bilirubin and total bile acids were elevated. (3) In the rats subjected to partial bile duct ligation, bile infarction and bile duct proliferation were seen only in the lobes with ligation of the hepatic ducts: only slight or no elevation of the serum levels of total bilirubin and total bile acids was found. These data suggest that bile infarction is caused by the toxic action of bile constituents other than bilirubin and bile acids, absorbed into the blood from the obstructed biliary system, and that bile duct proliferation is due to mechanical factors following bile retention or direct actions of retained bile in the liver.

ISSN:0022-3417    

DOI:10.1002/path.1711600112

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

摘要:

AbstractRats were exposed to clouds of the following pneumoconiotic dusts: quartz, coal‐mine dust, and chrysotile asbestos at l0 or 50mg/m3for 8, 32, and 75 days; for comparison, rats were also exposed to the non‐pathogenic dust titanium dioxide (TiO2). The bronchoalveolar leukocytes (macrophages and neutrophils) from dust‐exposed and control rats were obtained by lavage and tested for their ability to migrate toward zymosan‐activated serum. Varying amounts of neutrophils were present depending on the ability of the dust to cause inflammation and the length of exposure. There was a marked loss of chemotactic ability in leukocytes from rats inhaling the pneumoconiotic dusts compared with controls; TiO2‐exposed leukocytes had some impairment of chemotaxis, but this was substantially less than that found with the pneumoconiotic dusts. The loss of chemotactic activity did not correlate with the percentage of neutrophils in the lavage cells except when there were very high levels of neutrophils, and there was substantial impairment of chemotaxis with negligible numbers of neutrophils, showing that macrophage chemotaxis was impaired. A phagocytic burden within the leucocytes was not sufficient alone to inhibit chemotaxis, nor was the loss of chemotaclic activity due to occupied receptors, since incubation failed lo restore chemotaxis. Loss to chemotactic activity by leukocytes from pneumoconiotic dust‐exposed lung could be an important factor in the development of pne

ISSN:0022-3417    

DOI:10.1002/path.1711600113

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

摘要:

AbstractThree tumours which arose in two (one male and one hermaphrodite) out of 63 chimaeric mice resulting from injection of El4TG2a embryo stem (ES) cells into host blastocysts1have been investigated. All of the tumours appeared within the first 3 weeks after birth. The tumour in the male chimaera and one of the tumours in the hermaphrodite were in the perigenital region but were extragonadal. The third, smaller tumour in the hermaphrodite was on the caecum. The perigenital tumour in the male chimaera was a teratocarcinoma with a wide variety of differentiated tissues, including non‐pigmented retina, as well as nests of undifferentiated embryonal carcinoma (EC) cells with high levels of alkaline phosphatase activity. The perigenital tumour in the hermaphrodite was a teratoma, less differentiated and with no evidence of EC cells. Glucose phosphate isomerase isozyme analysis indicated that both perigenital tumours were predominantly of the injected ES cell rather than the host blaslocyst type. The possible origins of these tumours, which are the first reported to have arisen from ES cells in chimaeric mice, are discusse

ISSN:0022-3417    

DOI:10.1002/path.1711600114

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

摘要:

AbstractThe purification of many intracellular and extracellular inclusions is often difficult to achieve due to the low concentration of the abnormalities in the tissue under study, or due to the degradation of components during extraction. We describe the use of microdissection for the isolation of neurons and intraneuronal inclusion bodies. The resulting suspension may be used for biochemical, immunological or ultraslructural studies. The technique is applicable to the study of a wide range of cellular abnormalities.

ISSN:0022-3417    

DOI:10.1002/path.1711600115

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711600116

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711600117

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711600120

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711600121

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711600122

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711600124

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY