摘要:

AbstractLocalization of hepatitis C virus (HCV) antigens was studied in fresh frozen and formalin‐fixed, paraffinembedded liver tissue by immunoperoxidase using monoclonal antibodies to nucleocapsid protein and polyclonal human immunoglobulin G purified from plasma containing antibodies to structural and non‐structural antigens of hepatitis C virus. The results observed using monoclonal antibody to HCV core were similar to those of polyclonal IgG against HCV antigens in the majority of cases and both correlated well with HCV status as defined by ‘nested’ polymerase chain reaction. HCV antigens were detected in both hepatocytes and mononuclear cells. Using polyclonal human IgG, a small proportion of biliary epithelial cells were also positive in 6/29 patients. In most of the specimens examined, relatively few cells (1–5 per cent) were found to be positive for HCV antigens. The cryostat sections, using polyclonal IgG against HCV antigens, exhibited greater immunohistochemical staining, suggesting that the fixation and processing of the tissue may be a major factor in the conservation and the outcome of HCV antigen(s) findings. However, the results using monoclonal antibodies may reflect the specificity of antigen e

ISSN:0022-3417    

DOI:10.1002/path.1711750112

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

摘要:

AbstractA polymerase chain reaction (PCR) assay for the rapid and species‐specific diagnosis of mycobacterial infections in paraffin‐embedded clinical specimens was developed using oligonucleotide primers to amplify a fragment of the DNA coding for the ribosomal 16S RNA of mycobacteria. The oligonucleotide primers amplified DNA from all 14 species of mycobacteria tested. By means of a reamplification protocol, as few as one to two mycobacteria could be detected in the presence of human DNA. The method of DNA isolation and amplification was applied on sections of routinely formalin‐fixed and paraffin‐embedded tissues. PCR for the β‐actin gene served as a control for successful DNA isolation. Mycobacterial DNA could be detected in cases of mycobacterial infections. The mycobacterial species was determined by additional sequencing of the PCR fragment. This PCR method may be a powerful tool for the diagnosis of mycobacterial infections from histopathological material and for the assessment of those mycobacteria that cannot readily be cultured, such asMycobacter

ISSN:0022-3417    

DOI:10.1002/path.1711750113

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711750114

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711750115

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711750118

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711750119

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711750101

出版商:John Wiley&Sons, Ltd.    

年代:1995

数据来源: WILEY