ISSN:0022-3417    

DOI:10.1002/path.1711650202

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractThis study sought to determine, firstly, the relative frequency of lymphocytes and macrophages and, secondly, the percentage of lymphocytes containing interferon‐γ in inflamed islets (insulitis) of patients with type 1 (insulindependent) diabetes. Autopsy pancreases of 12 patients who had died of recent‐onset type 1 diabetes and one prediabetic patient who had died of cardiomyopathy were examined immunohistochemically. In the 87 islets that were studied, the lymphocyte macrophage ratio was 9·7:1 and approximately 40 per cent of the lymphocytes contained interferon‐γ. Interferon‐γ release in the insulitis process may be involved in the pathogensis of type

ISSN:0022-3417    

DOI:10.1002/path.1711650203

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractHuman insulin and glucagon mRNA were identified in routinely processed pancreatic tissue by non‐radioactivein‐situhybridization using digoxigenin‐labelled oligoncleotide probes. Cocktails of synthetic oligonucleotides complementary to human insulin and glucagon mRNA were labelled with digoxigenin using terminal deoxynucleotidyl transferase (Tdt). Specific hybrids were detected with alkaline phosphatase‐labelled anti‐digoxigenin antibody and visualized by BCIP‐nitroblue tetrazolium indicator substrate. The results showed highly sensitive and specific staining of islet cells on a range of routinely formalin‐fixed and paraffin‐embedded tissues. Post‐mortem pancreatic tissue from adults and stillborn neonates yielded acceptable signals as long as tissue morphology was well preserved. Preliminary investigations using pancreatic endocrine cell tumours gave clear easily interpretable signals which were comparable to conventional immunostaining. The application of this technique promises to be of value in the investigation of p

ISSN:0022-3417    

DOI:10.1002/path.1711650204

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractThe distribution and expression of preproparathyroid hormone (PTH) mRNA were investigated in parathyroid tissue from 57 parathyroidectomy specimens. PTH mRNA was detected byin situhybridization using digoxigeninlabelled oligonucleotide probes. Cell morphology was seen to correlate with PTH mRNA expression. Strong expression of PTH mRNA was confined to cells which on haematoxylin and eosin staining had large vesicular nuclei. These included both vaculated and non‐vacuolated cells. Chief cells with small dark nuclei and scanty cytoplasm had little or no expression. In both adenoma and chief cell hyperplasia, the striking difference from normal was the greatly increased proportion of cells expressing PTH mRNA. In adenomas, the rim of uninvolved parathyroid tissue showed PTH mRNA expression similar to that of normal parathyroid. In hyperplasia, there was frequently concordance of staining within individual nodules. The findings establish morphological criteria for activity of parathyroid tissue and support current concepts of the different pathogenesis of hyperplasia and adenoma. The expression of PTH mRNA in oxyphil change and parathyroid carcinomas was also investigate

ISSN:0022-3417    

DOI:10.1002/path.1711650205

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractTwo adjacent antral biopsies were taken endoscopically from 41 patients attending a clinic for the investigation of upper gastrointestinal tract symptoms. One was processed for histopathological grading and assessment of theHelicobacter pyloriby light microscopy. The other was fixed in tannic acid, which demonstrates cell leakiness, and processed for electron microscopy. On each grid, the light (electron lucent) and dark (electron dense—leaky) gastric superficial and foveolar cells away from mechanical trauma of biopsy were counted, along with the number ofH. pyloriwhich were in contact with the epithelial cells.There was a significant relationship between histological grade of gastritis and the extent of electron microscopic damage, i.e., the degradation of normal to leaky cells and the number ofH. pyloriper leaky cell.If the patients were divided into two groups with minimal and maximal gastritis and compared against each other, then there was a significant difference between the two groups with respect to surface cell damage, polymorphs per unit area, the number of glands invaded by polymorphs, light/dark cells,Helicobactergrades assessed light microscopically or totals counted in contact with epithelial cells by electron microscopy, the number ofHelicobacterper cell, and the age of the patients. There was no difference between the two groups on their endoscopic appearances or treatment with H2 blockers or NSAID

ISSN:0022-3417    

DOI:10.1002/path.1711650206

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractThe leucocyte antigen CD23 is upregulated in the early stages of B‐cell activation by Interleukin‐4 (IL‐4), and functions as an IgE receptor and lymphocyte growth factor. We have studied the expression of CD23 in 68 cases of non‐Hodgkin lymphoma (NHL) using the antibody BU38. This new antibody has the great advantage of being applicable to routinely‐processed paraffin sections. CD23 was expressed in tumour cells in 27 out of 36 cases of low grade NHL and 3 out of 32 cases of high grade NHL. Follicular dendritic cells were strongly positive and were seen in follicular lymphomas. Macrophages were also positive and were numerous in high grad

ISSN:0022-3417    

DOI:10.1002/path.1711650207

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractThis study quantified the constitutive and interferon‐gamma (IFN‐γ) stimulated expression of MHC class I (HLA‐ABC and β2, microglobulin) and class I1 antigens (HLA‐DR, ‐DP, ‐DQ) on normal and malignant oral keratinocytes using radioimmunoassay and immunocytochemical techniques. Normal keratinocytes and three of four malignant cell lines (H 103, H1 57, H314) expressed MHC class I antigens constitutively; IFN‐γ increased MHC class I expression with significant changes in normals, H157 and H314. Normal keratinocytes expressed significantly more constitutive MHC class I antigens than H103 and H157 and significantly more IFN‐γ stimulated MHC class I antigens than H103, H157 and H314. MHC class II antigens predominantly were not expressed constitutively on normals, H103 and H157 but, in H314, HLA‐DR, ‐DP and ‐DQ antigens were demonstrated on 35, 11 and 5 per cent of cells, respectively, and resulted in a non‐coordinated pattern ofexpression (HLA‐DR>‐DP = ‐DQ). IFN‐γ induced HLA‐DR on normals, H103 and H157, whilst HLA‐DP and ‐DQ remained undetectable. In H314, IFNγ enhanced HLA‐DR, ‐DP and ‐DQ (significant increase of HLA‐DQ) but the interrelationship between these antigens was maintained (HLA‐DR>‐DP = ‐DQ). Normal keratinocytes expressed significantly more IFN‐γ stimulated HLA‐DR than H103 and H157 but significantly less HLA‐DR than H314 under similar experimental conditions. One oral malignant cell line (H191) did not express MHC class I and MHC class II antigens either constitutively or in response to IFN‐γ. The results demonstrate aberrant patterns of MHC expression (absence, enhanced,

ISSN:0022-3417    

DOI:10.1002/path.1711650208

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractIn pleural of ascitic effusions the cytomorphological distinction of adenocarcinoma cells, reactive mesothelial cells, and malignant mesothelioma cells often causes a diagnostic dilemma. The value of immunocytochemistry was investigated on cytological smears of 24 well‐established cases of malignant mesothelioma, a selected series of 31 metastatic adenocarcinomas, and 20 smears of patients without known malignancy. In these smears we scored the immunoreactivity with a panel of four monoclonal antibodies. In addition to antibodies for epithelial membrane antigen (EMA) and carcinoembryonic antigen (CEA), the monoclonal antibody MOC31 and the ovarian carcinoma specific antibody OV632 were incorporated in the panel. With none of these four antibodies was immunostaining of reactive mesothelial cells found.CEA‐ and MOC31‐positive tumour cells were frequent in metastatic adenocarcinomas, but occurred rarelay in malignant mesothliomas. EMA‐positive tumour cells were in all metastatic adenocarcinomas (100 per cent) and in most malignant mesotheliomas (83 per cent). In addition to the expected reactivity of OV632 with ovarian carcinomas, 22 of 24 malignant mesotheliomas contained immunopositive tumour cells, while only a small proportion of non‐ovarian adenocarcinomas reacted with this antibody.This selective staining of malignant mesothelioma cells, but not reactive mesothelial cells, with OV632 now permits the positive identification of malignant mesothelioma cells in male

ISSN:0022-3417    

DOI:10.1002/path.1711650209

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractThe immunoreactivity of OV‐TL 12/30, a monoclonal anti‐keratin 7 antibody (Mab), was investigated on frozen as well as paraffin‐embedded human tissues. Its reactivity patterns were compared with another well‐characterized monoclonal antibody to keratin 7 (RCK 105), and with broadly cross‐reacting monclonal (OV‐TL 12/5) as well as polyclonal (pKer) keratin antisera. In frozen sections of normal and malignant human tissues both keratin 7 Mabs gave similar staining patterns. The immunoreactivity for OV‐TL 12/30 and the polyclonal antibody (pKer) in tissue sections fixed in 4 per cent formalin or Bouin solution, was completely restored when pretreated with 0·1 per cent pronase, 0·1 per cent trypsin in phosphate‐buffered saline (PBS) or with 0·5 per cent pepsin in 0·01 N HCl. Except for loss of immunoreactivity on human normal stomach surface epithelium and glandular mucous cells, Mab OV‐TL 12/30 reacted strongly positive with essentially all those formalin‐ or Bouin‐fixed paraffin‐embedded tissues that had been shown to stain in non‐fixed, frozen sections. In addition to the good correlation in normal human tissues, a complete correlation between the reactivity on frozen and paraffin‐embedded human carcinomas (n= 86) was found as well. While both RCK 105 (anti‐keratin 7) and OV‐TL 12/5 (anti‐keratin 5, 7, 14, 19) did not stain on paraffin‐embedded sections, the polyclonal control antiserum (pKer) lost immunoreactivity in some cell types (e.g. mucous cells in compound glands, hepatocytes, pancreatic acinar cells, and proximal and distal convoluted tubules of the kidney). Our study shows that the keratin 7 Mab OV‐TL 12/30 is an excellent marker for tumour histopathology since it is reactive in paraf

ISSN:0022-3417    

DOI:10.1002/path.1711650210

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractWe have produced a range of monoclonal antibodies which stain human intrahepatic bile ducts of different sizes. Amongst 26 monoclonal antibodies produced, five clones reacted specifically with bile ducts of different sizes, of which three have been maintained in culture and their viability following freezing and thawing confirmed. Staining patterns varied between normal adult liver tissue, normal fetal liver tissue and a variety of hepatobiliary diseases. The antibodies provide further evidence of the immunological heterogeneity of the human intrahepatic biliary tree and support the hypothesis that proliferating bile ductules are derived from periseptal hepatocytes. The preparation of the antiboides, their staining reactions in normal adult, normal fetal and a variety of liver diseases are described.

ISSN:0022-3417    

DOI:10.1002/path.1711650211

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY