ISSN:0022-3417    

DOI:10.1002/path.1711570302

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711570303

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractThe nuclear tumour antigen p53 is expressed by a gene localized on the p‐arm of human chromosome 17, a region frequently deleted in colon carcinomas. Using a monoclonal antibody to p53 antigen, immunohistochemical analysis of carcinomas and dysplastic tubular adenomas of the colon has been performed to study the relation between p53 expression and dysplasia or malignancy. With this methods p53 was detectable in 55 per cent of colon carcinomas (n = 29).In 8 per cent of adenomas (n = 74), focal nuclear p53 expression was found in dysplastic epithelial cells. In general, these p53‐positive regions of the polyps were histologically indistinguishable from the neighbouring tubuli. Sometimes the p53‐positive nuclei were found in a focus of more highly dysplastic epithelium. The results suggest that expression of the p53 gene may be part of the process of malignant transformation of dysplastic colon p

ISSN:0022-3417    

DOI:10.1002/path.1711570304

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractAdoptive immunotherapy with lymphokine‐activated killer (LAK) cells and systemic administration of recombinant Interleukin‐2 (RIL‐2) was carried out in a case of malignant melanoma with lung metastases. Histological specimens from the lung showed a metastatic melanoma heavily invaded by atypical lymphoid cells with convoluted nuclei of varying size. Immunohistochemistry revealed that these cells had the characteristic exclusively of natural killer cell (Leu‐7+). Nodules of these cells mimicked the appearance of non‐Hodgkin's lymphoma of pleomorphic type. Molecular cytogenetic analysis, however, showed the absence of rearranged bands for the T‐cell receptor beta‐chain gene, indicating the absence of T‐cell clones. At autopsy, 1 month after the LAK therapy, the heavy invasion of convoluted cells had disappeared. These findings clearly indicate that the LAK cell plus RIL‐2 therapy induced Leu‐7+lymphoid cells, phenotypically suggestive of natural killer cell aggrega

ISSN:0022-3417    

DOI:10.1002/path.1711570305

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractAnin vitroassay was used to test whether the patterns of lymphocyte distribution in the mouse skin could be explained by adhesion of lymphoid cells to connective tissue. Using normal skin as a substrate,in vitroadhesion was significantly greater below the panniculus carnosus muscle: when oxazolone sensitized skin was used, binding was seen throughout the skin. This correlated closely with the patterns seenin vivofollowing injection of lymphocytes into normal skin or sensitization with oxazolone. Adhesion was not affected by divalent cation chelating agents, but was reduced in the presence of some monosaccharides. The property of skin adhesion did not appear to be confined to a small number of cells in the spleen cell preparation. Although only a small proportion of the starting population adhered to the section, transfer of the non‐adherent cells to a fresh skin section produced a similar degree of adhesion.It is suggested that the variable binding of lymphoid cells by skin connective tissue may be an important factor in determining the retention of cells which have emigrated into the skin from the blood vessels and may explain the distinctive patterns of distribution of lymphocytes in cutaneous inflammatory disorder

ISSN:0022-3417    

DOI:10.1002/path.1711570306

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractCell/cell contacts in the intact media of the rat thoracic aorta and inferior vena cava, and in cultured smooth muscle cells from these vessels have been quantified in order to determine whether their density and their type can be correlated with the differences in load at these two sites. The type and number of contacts per 100 μm of cell perimeter and per 100 cells were determined with a semi‐automatic image analysis system. In all cases there was a predominance of simple appositions; intermediate junctions, interdigitations, and nexus junctions were less frequently observed. There were more cell contacts of each type in the intact arteries than in the intact veins. In cell cultures, all types of cell contacts were increased but the proportion of load bearing types was reduc

ISSN:0022-3417    

DOI:10.1002/path.1711570307

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractFifty‐four cases of invasive carcinoma of breast were immunostained for fibronectin and laminin. They included 36 cases of invasive ductal carcinoma and 18 cases of invasive lobular carcinoma. Although there was some heterogeneity within tumours, it was found that whilst the majority of ductal carcinomas (31/36) had abundant fibronectin at cell/stroma boundaries or diffusely throughout stroma, a substantial proportion of lobular carcinomas (12/18) had very little (P<0.001). This difference could not be related to differences in laminin immunoreactivity, which was most commonly scanty or absent in both tumour types. It is postulated that the characteristic infiltration pattern of lobular carcinoma may be attributed in part to paucity of stromal fibronecti

ISSN:0022-3417    

DOI:10.1002/path.1711570308

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractTesticular Leydig cell tumours are able to produce oestrogens and can be induced by exogeneous oestrogen administration. Oestrogen and progestrone receptors, cytokeratin, vimentin, and proliferative activity were determined immunohistologically in human testes in six Leydig cell tumours, 14cases of Leydig cell hyperplasia, and 13 cases with normal Leydig cells. While both steroid receptors were detected in about 70 per cent of the tumour cells in cryostat sections, no reaction was observed in normal Leydig cells. This supports the hypothesis of an enhanced receptor state in a Leydig cell subpopulation as a basic pathophysiological factor in the development of Leydig cell tumours. On cryostat sections, all tumours co‐express cytokeratin and vimentin. Neither the receptors nor the intermediate filaments could be detected reliably in paraffin sections. The low proliferative activity of Leydig cell tumours corresponds to their benign clinical cours

ISSN:0022-3417    

DOI:10.1002/path.1711570309

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractThe human gastric fundal mucosa contains a variety of endocrine cells, the most numerous of which are the so‐called enterochromaffin‐like (ECL) cells. We have studied the variations with age and sex of the ECL cell populations, utilizing an assessment based on multiple endoscopic biopsies from four groups of subjects. Plasma gastrin levels were also determined in these subjects.In males, endocrine cell densities declined with age but the ECL cell numbers in females opposed this trend. ECL cell counts showed no appreciable differences between young and old females. In older females, there was a high rate of gastritis and increased levels of circulating gastrin. Concentrations in older females (29.6±8.7 pmol/l) were higher than in both younger (0.05). The plasma gastrin level was also higher in older females than in young females (13.1±4.5 pmol/l), although this difference failed to reach statistical significance.In conclusion, clinically silent gastritis, raised gastrin levels, and maintenance or rise of ECL cells numbers, in opposition to a general decrease in endocrine cells with age, appear to be features of women of more than 55 years of age. The variations in ECL cell populations reported here should be taken into account when evaluating possible pathological alterations of the

ISSN:0022-3417    

DOI:10.1002/path.1711570310

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractA monoclonal antibody (LH 7.2) to the carboxy terminal of type VII collagen has been used in an indirect immunoperoxidase method on frozen sections of biopsies of benign compound and intradermal cellular naevi, dysplastic naevi, and malignant melanomas, to label the epidermal basement membrane. Benign intradermal naevi had an intact membrane with the dermal naevus cell component lying beneath it. Compound naevi showed proliferating junctional nests of naevus cells pushing through the membrane towards the dermis beneath. In contrasts, dysplastic naevi and melanomas showed a progressive displacement of the membrane downwards. Thin lesions were confined above the membrane. With increasing tumour thickness, discontinuities in the membrane were seen and foxi of invasive growth through the line of the membrane were identified. These studies suggest that the good prognosis of thin melanomas is associated with confinement of the tumour within an expanded epidermis above the basement membrane.

ISSN:0022-3417    

DOI:10.1002/path.1711570311

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY