ISSN:0022-3417    

DOI:10.1002/path.1711590302

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711590303

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractIsolated single myocytes were prepared from myocardium of developing ventricular aneurysms and from myocardium within the scar of chronic ventricular aneurysms. The morphology and function of the individual cells were compared. The cells from developing aneurysms were rod‐shaped, with a distinct sarcomeric structure, but did not contract even in the presence of high calcium concentrations. The sarcomere length was significantly higher than that of cells from chronic aneurysms and approached the theoretical point at which no contraction can occur. Cells from chronic aneurysms were either rod‐shaped and contractile, or rounded due to hypercontracture of the myofilaments. Electron microscopy of cells from developing aneurysms confirmed the presence of elongated sarcomeres, a loss of the actin‐myosin interdigitation, and damage to the contractile proteins which was particularly evident in the thin filaments. Cells with similar characteristics have also been isolated from a ruptured, ischaemic papillary muscle. These changes, which are due either to ischaemia or to overstretching of cells, may account for the weakness of the wall of developing aneurysms and be a cause of rupture or enlarg

ISSN:0022-3417    

DOI:10.1002/path.1711590304

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractWe performed immunological phenotyping of mononuclear cells in tissue sections of 84 endomyocardial biopsies (EMB) showing infiltrates, which were taken from 21 patients after heart transplantation. Data were correlated with histology (grading following Billingham) and cyto‐immunologic monitoring (CIM) on blood samples (grading into negative, rejection, or infection, based on leukocyte morphology and T‐cell phenotype). Few T lymphocytes were observed in 35 biopsies, and many in 49 biopsies. The semi‐quantitative estimate of T cells and CD4/CD8 ratio did not correlate with EMB histology but was related to CIM data. For example, 31 out of 42 cases with a CIM indicative of infection (14 of which showing no rejection on histology) manifested large numbers of T cells. In most cases, the CD4/CD8 ratio was less than 1. The presence of activated cells (bearing interleukin‐2 receptors or the CD30 antigen) was not related to EMB histology or to CIM data. The number of T cells (subsets) in EMB was not related to relative or absolute numbers in th

ISSN:0022-3417    

DOI:10.1002/path.1711590305

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractOf 723 infertile men (128 with a history of cryptorchidism) whose testes were biopsied at the outer lateral face of the testis, five presented carcinomain situ(CIS) in one testis. These testes were removed, serially sectioned, and examined by light microscopy. In order to evaluate whether only one or two biopsies are sufficient to diagnose CIS, before sectioning the testes four biopsies were taken at the anterior face, posterior face, superior pole, and inferior pole of the testis, respectively. Two of the five men had undergone orchiopexy in infancy and the testis contained tubules with Sertoli cells and isolated spermatogonia. CIS was also present in some tubules that were principally located near the rete testis. Of the four simulated biopsies, only that performed at the posterior face of the testis revealed CIS. The other three infertile men showed tubules with complete, although reduced, spermatogenesis, and tubules lined by Sertoli cells only. CIS was found in both types of tubules. These tubules with CIS formed lobules that extended throughout the testicular parenchyma. Most simulated biopsies performed in these three testes showed CIS. The average nuclear DNA content of CIS cells was about 4c in all testes. This content was similar both in tubules with complete spermatogenesis and in tubules with Sertoli cells only.

ISSN:0022-3417    

DOI:10.1002/path.1711590306

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractImmunophenotyping shows heterogeneity of expression of activation and differentiation antigens in B‐cell non‐Hodgkin's lymphoma (NHL). To investigate whether antigen expression correlates with clinical behaviour we have studied the clinical presentation and follow‐up of a series of 111 B‐cell lymphomas previously phenotyped for a panel of antigens including CD groups 5, 9, 10, 21, 23, 25, 30, 38, 4F2 antigen, and transferrin receptor. CD antigens 5, 10, and 23 were expressed significantly more often by low grade lymphomas whereas CD38, 4F2 antigen, and transferrin receptor were more often expressed by high grade lymphomas. There was a significant correlation with survival and age, stage at presentation, histological grade, and expression of 4F2 antigen and transferrin receptor but not with the other antigens studied. 4F2 antigen and transferrin receptor may identify a poor prognostic group of cases in low grade lymphoma but we conclude that phenotyping B‐cell NHL for many of the antigens expressed at various stages of B‐cell differentiation and activation does not provide clinically useful information in addition to that obtained from standard histological clas

ISSN:0022-3417    

DOI:10.1002/path.1711590307

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractSudden natural deaths of individuals between the ages of 2 and 20 years which occurred during a 20‐year period were identified from mortuary records. Necropsy reports and histological sections were reviewed; 169 sudden natural deaths were identified amongst 1012 deaths in that age group. Ninety‐two sudden deaths occurred to children with recognized disorders; congenital heart disease, asthma, and epilepsy were the commonest problems identified. Amongst the 77 deaths of apparently healthy children, infection was the most frequently recognized disease. Only 11 deaths were unexplained, comprising 1 per cent of the necropsy population or 6·5 per cent of sudden natural deaths, a much smaller proportion than pertains in infancy. Necropsy examination of children dying suddenly yields useful informa

ISSN:0022-3417    

DOI:10.1002/path.1711590308

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractThe distribution of tenascin, an extracellular matrix protein highly expressed in the stroma around sites of epithelial‐mesenchymal interaction during morphogenesis and in malignant neoplasms, was assessed in cryostat sections of 17 basal cell carcinomas using a polyclonal antibody. There was marked staining of the vascularized stroma around neoplastic islands, usually as an intense, well‐defined band, but being more widespread and diffuse in sclerosing, infiltrative areas. Apparently enhanced staining was seen in tumours showing retraction artefact, which may be related to the observation that tenascin interferes with the cell binding function of fibronectin. Reduced staining was seen in areas showing evidence of tumour regression. Tenascin is an important component of the epithelial‐mesenchymal interactive process and further studies on its distribution in benign and malignant skin tumours are req

ISSN:0022-3417    

DOI:10.1002/path.1711590309

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractGastric cellular and humoral immune responses investigated by immunoperoxidase staining of 53 antral biopsies showed significant differences inCampylobacter pylori‐associated gastritis as compared with non‐bacterial chronic gastritis and normal controls. IgA, secretory component, and complement C3 coated bacilli were seen in all cases of active chronic gastritis. C3 was always associated with coating by IgA, IgM, or both, which were rarely seen in gastritis without polymorphonuclear neutrophil infiltration. Intraepithelial mononuclear cellular infiltration was seen in 18 of 26 cases of C. pylori‐associated chronic gastritis. The intraepithelial mononuclear cells stained positively for T cells and histio

ISSN:0022-3417    

DOI:10.1002/path.1711590310

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractThe distribution of pregnancy‐associated prostaglandin synthetase inhibitor (PAPSI) has been studied in 181 human placentae and early products of conception using a peroxidase‐antiperoxidase (PAP) immunohistological method. PAPSI was detected in amniotic epithelium throughout all stages of gestation. Extravillous trophoblast was PAPSI‐positive throughout gestation. Villous trophoblast was PAPSI‐positive in the first 12 weeks of gestation, when PAPSI was localized to syncytiotrophoblast but not to cytotrophoblast. Villous syncytio‐and cytotrophoblast were both PAPSI‐negative from 12 weeks to term. Fetal and maternal polymorphs contained PAPSI at all stages of gestation, as did villous Hofbauer cells. The significance of these findings is discussed in relation to putative functions of PAPSI, particularly the maintenance of pregnancy and control of onset of

ISSN:0022-3417    

DOI:10.1002/path.1711590311

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY