摘要:

AbstractUsing a panel of monoclonal antibodies against a variety of lymphoid and non‐lymphoid antigens the immuno‐histological staining pattern of giant cells from a case of giant‐cell tumour of bone has been compared with that of osteoclasts from the developing ends of fetal long bones. Only EBM‐11, an antibody reacting with a wide spectrum of macrophages, stained both osteoclasts and giant cells; stromal cells and osteoblasts did not react. This indicates that osteoclasts and giant cells are phenotypically and presumably functionally similar. It is argued that the osteoclasts and the tumour‐derived giant cells in bone are derived from a similar mononuclear

ISSN:0022-3417    

DOI:10.1002/path.1711470302

出版商:John Wiley&Sons, Ltd.    

年代:1985

数据来源: WILEY

摘要:

AbstractThe immunohistochemical localization of basement membrane type IV collagen was investigated with a mouse monoclonal antibody in major surgical resections from 25 patients with invasive squamous carcinomas of the head and neck. Irrespective of site, size or stage of the disease, the 16 primary invasive tumours were almost completely surrounded by a layer of type IV collagen. Focal abnormalities were regularly present, consisting of thickening and aggregation of type IV collagen together with attenuation and segmental loss. Similar changes were seen in metastatic squamous carcinomas in 36 cervical lymph nodes. It is suggested that the probable formation of a normal basement membrane protein by these squamous carcinomas indicates the preservation of a normal function of differentiating squamous epithelia. The results indicate that a major basement membrane component, type IV collagen, continues to co‐exist with invasive and metastatic squamous carcinoma

ISSN:0022-3417    

DOI:10.1002/path.1711470303

出版商:John Wiley&Sons, Ltd.    

年代:1985

数据来源: WILEY

摘要:

AbstractThe epithelial cell strain NAL1A cultured from normal adult mouse lung has been transformed by culturing in dexamethasone into an invasive neoplastic cell strain. The criteria for neoplastic transformation include the capacity for anchorage independent growth in soft agar as well as the formation of invasive neoplastic nodules after subcutaneous transplantation in thymectomized irradiated newborn mice. The cells of the invasive neoplastic nodules induced by dexamethasone culturing of NAL1A were indistinguishable hislopathologically and by electron microscopy from invasive nodules evoked by the subcutaneous inoculation of CMT64, a cell line cultured from a metastasizing mouse lung tumour and cell strain NUL1 derived from mouse pulmonary adenomata induced by urethane. Cells of the nodules derived from all three cultured strains possessed desmosomes, surface microvilli and phospholipid lamellar bodies characteristic of the type 2 pneumocyte. It is concluded that cultured cell strains NAL1A, cultured in dexamethasone, NUL1 and CMT64 evoke invasive subcutaneous neoplasms derived from a common ancestor, presumably a type 2 pneumocyte related stem cell.

ISSN:0022-3417    

DOI:10.1002/path.1711470304

出版商:John Wiley&Sons, Ltd.    

年代:1985

数据来源: WILEY

摘要:

AbstractUlex europeus(Ulex 1) has been used to study the expression of cell surface α‐L‐fucose on oral epithelial cells of normal human mucosa, white lesions and carcinomas. In normal tissue and in 11 of 12 specimens of non‐specific keratoses (leukoplakia), Ulex 1 stained epithelial spinous cells only. In 16 specimens of oral lichen planus, Ulex 1 labelled spinous epithelial cells, and in 12 there was also staining of the basal epithelial cells. Two specimens of white sponge naevus showed a total absence of epithelial staining with Ulex 1. Squamous carcinomas showed a loss of staining in the invading epithelial islands in six of eight specimens, but normal labelling of the surfaces of spinous cells at sites distant from the areas of epithelial invasion. When the severity of epithelial dysplasia, as assessed by the index of epithelial atypia, was compared with the pattern of staining of Ulex 1 in normal mucosa and white lesions there was no correlation. The results show that failure of Ulex 1 to stain cell surface carbohydrates may not be attributable to malignant transformation. The staining of cells not normally stained may be related to hitherto masked residues revealed by cell damage or altered different

ISSN:0022-3417    

DOI:10.1002/path.1711470305

出版商:John Wiley&Sons, Ltd.    

年代:1985

数据来源: WILEY

摘要:

AbstractA qualitative and quantitative histological study was made of the carotid bodies obtained at necropsy from 47 subjects ranging in age from 14 to 100 years. With increasing age there is a loss of more than a third of the amount of glomic tissue with a diminution in the size of the lobules. In young people the basic histological pattern of clusters, composed of cores of chief cells with surrounding rims of sustentacular cells, has commonly superimposed on it prominence of the dark variant of chief cells. In middle‐aged subjects there is proliferation of sustentacular cells which appear to merge imperceptibly into fibrosis of the lobules and becomes associated with diffuse or focal infiltrates of lymphocyte

ISSN:0022-3417    

DOI:10.1002/path.1711470306

出版商:John Wiley&Sons, Ltd.    

年代:1985

数据来源: WILEY

摘要:

AbstractMesangial uptake and disposal of antigen‐coated latex particles and the ability of subsequently injected antibody to maintain complexed antigen in the rat mesangium has been investigated. Carboxylate‐modified latex particles, coated with bovine albumin (BSA) were injected i.v. to 36 Wistar rats. Twenty‐two rats (group 1) were not treated further. Fourteen rats (group 2) received rabbit anti‐BSA antiserum i.v. and i.p. 24 h later. Control groups were injected with uncoated, unmodified latex particles or soluble BSA with and without subsequent antibody administration. Latex was present in the mesangial matrix of rats in group 1 at 1 h in association with a diffuse mesangial distribution of BSA. At 24 h, BSA staining was markedly reduced and extracellular latex was no longer observed. Intracellular latex aggregates were present in experimental and control groups at 24 h‐14 days in cytoplasmic vacuoles of hypertrophic mesangium which showed minor infiltration by macrophage‐like cells. Progressive removal of latex aggregates coincided with declining mesangial reactivity. Rapid disappearance of antigen apparently results from local degradation of tracer in the mesangium. Antibody administration preserves BSA in the mesangium due to immune complex formation and is associated with retention of ingested latex by mesangial cells. However, efficient disposal of glomerular immune deposits by the mesangium appears to minimize infiltration by monocytes and prevents aggravation of glomerular i

ISSN:0022-3417    

DOI:10.1002/path.1711470307

出版商:John Wiley&Sons, Ltd.    

年代:1985

数据来源: WILEY

摘要:

AbstractThe organs of monkeys infected with Ebola haemorrhagic fever were examined by light and electron microscopy during the acute stage of the disease. The virus caused focal coagulative necrosis in the liver, spleen, kidney, lung and testis and widespread mild vascular damage. In the brain there was intense congestion, with erythrocyte ‘sludging’, but no inflammatory reaction. There was significant injury to the microvasculature in all organs. Virus replicated in endothelial cytoplasm causing focal necrosis, separation of tight junctions and detachment from basement membranes. These changes were associated with oedema and haemorrhage, but though contributing to the hypovolaemic shock were not sufficiently extensive to account for the severity of vascular collapse. Renal involvement was also clinically important. Some renal cellular injury was caused by direct virus invasion ofglomerular endothelium and tubular epithelium, but much tubular damage was probably due to ischaemia resulting from thrombosis in the peritubular capillaries. The virus also replicated in lymphocytes and monocytes and in interstitial cells of the testis. Since particles were not found in seminiferous epithelium, the degeneration of spermatogonia and spermatocytes was probably secondary to ischae

ISSN:0022-3417    

DOI:10.1002/path.1711470308

出版商:John Wiley&Sons, Ltd.    

年代:1985

数据来源: WILEY

摘要:

AbstractThirty‐nine cases of primary small intestinal lymphoma were studied by morphological and immunohistochemical methods. The adjacent uninvolved mucosa was also examined for features suggestive of coeliac disease. Employing the immunogold silver staining (IGSS) technique and polyclonal primary antisera against α‐l‐antitrypsin, lysozyme and κ and λ light chains, 29 cases (74·5 per cent) were found to be B‐cell lymphomas, seven (18 per cent) histiocytic tumours, one (2·5 per cent) Hodgkin's disease and two (5 per cent) remained unclassified. No specific lymphoma subtype was found to be associated with cases having the histological features of coeliac disease in the uninvolved adjacent mucosa. In 35 cases sufficient clinical information was available to assess the significance of histological type and stage in relation to survival. Although the histological type did not correlate with survival, stage did and tumours extending beyond the local lymph nodes were associated with a significantly wor

ISSN:0022-3417    

DOI:10.1002/path.1711470309

出版商:John Wiley&Sons, Ltd.    

年代:1985

数据来源: WILEY

摘要:

AbstractThe effects of various exogenous agents on subcellular structures is of importance to many investigators and can be critically evaluated by the use of cytochemical techniques and transmission electron microscopy. Therefore various cell culture techniques have become increasingly important in biological research in order to help determine these effects. Presently, most existing methods for processing anchorage‐dependent cultures for electron microscopy utilize cells grown on either glass or plastic substrates. Therefore various coating substances have been applied to the culture surfaces to facilitate removal of the sample, however, incomplete separation and sample fracture often results. Here we present a simple method ofin situprocessing of samples for electron microscopy involving the use of detachable chamber slides. This method allows for the use of a quick processing procedure that results in a complete separation of the sample from the glass slid

ISSN:0022-3417    

DOI:10.1002/path.1711470310

出版商:John Wiley&Sons, Ltd.    

年代:1985

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711470311

出版商:John Wiley&Sons, Ltd.    

年代:1985

数据来源: WILEY