ISSN:0022-3417    

DOI:10.1002/path.1711610102

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711610103

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

摘要:

AbstractAn immunohistochemical study of c‐erbB‐2 expression was carried out onin situ(non‐invasive) breast carcinoma, using antibody 21N, raised to the intracytoplasmic domain of the c‐erbB‐2 oncogene product. Strong membrane staining was observed in 44 out of 74 (59 per cent) cases of ductal carcinomain situ(DCIS), but none of 48 lobular carcinomain situ(LCIS) lesions. A detailed comparative morphological evaluation using several different parameters, including histological subtypes, was performed within the DCIS group.The results showed that there was a significant correlation between c‐erbB‐2 expression and the presence of large cell size, periductal lymphoid cell infiltration, marked nuclear pleomorphism, multinucleation, and a high mitotic rate. Of these, cell size appears to be the most important predictor of c‐erbB‐2 status, followed by the presence of periductal lymphoid cell infiltration.These results indicate, firstly, that LCIS and DCIS are biologically (as well as histologically) different and, secondly, that a subgroup of DCIS, which is associated with c‐erbB‐2 over‐expression, exists and appears to have distinct histological features. The subgroup of DCIS cases which over‐express c‐erbB‐2 may be a biologically definable category with prognostic importance. These results may therefore have relevance to breast screening programmes, but a larger study incorporating clinical data would be necessary to correlate these f

ISSN:0022-3417    

DOI:10.1002/path.1711610104

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

摘要:

AbstractThe c‐erbB‐2 proto‐oncogene encodes a 190 kMrprotein representing a putative growth factor receptor with considerable homology to the EGF receptor. Gene amplification and overexpression of the oncogene protein have been demonstrated in a variety of tumours including breast cancer, where it is associated with a poor prognosis.In this study we have produced and characterized a mouse monoclonal antibody, designated NCL‐CB11, to thec‐erbB‐2 protein. NCL‐CB11 was generated to a synthetic peptide sequence corresponding to a site of predicted antigenicity near the C‐terminus of the internal domain of the protein. NCL‐CB11 produces intense membrane‐associated immunohistochemical staining in a proportion of human cancer cells. The specificity of the antibody is supported by Western blotting and immunoprecipitation studies. Reactivity with an internal site of the protein is confirmed by the necessity of cell permeabilization for reactivity in fluorescence‐activated cell sorter(FACS) analysis. A high degree of correlation between immunohistochemical staining using NCL‐CB11, and c‐erbB‐2 gene amplification has been observed.NCL‐CB11 should prove to be a valuable reagent for investigations into the pathological significance o

ISSN:0022-3417    

DOI:10.1002/path.1711610105

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

摘要:

AbstractParathyroid hormone‐related protein (PTHrP) is invoked as the cause of humoral hypercalcaemia of malignancy (HHM); it is contained in the keratinocyte layer of normal skin; and there is evidence that is is produced by fetal parathyroids. Antibodies against synthetic PTHrP peptides have been raised in rabbits and sheep. This immunohisto‐chemical study has found that primary parathyroid adenomata and hyperplastic glands from patients with chronic renal failure stain positively with antisera against PTHrP(1–34) and PTHrP(50–69). Primary hyperplastic glands are negative. No staining with anti‐PTHrP(106–141) antiserum could be detected immunohistochemically in any of the parathyroid adenomata or

ISSN:0022-3417    

DOI:10.1002/path.1711610106

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

摘要:

AbstractUsing immunocytochemical techniques on fresh surgical samples, a series of 16 cases of cutaneous malignant melanoma (CMM) were examined to characterize further the host inflammatory response. Antibodies to the following cluster of differentiation (CD) antigens were used: CD‐3, CD‐4, CD‐8 (T‐cell markers), CD‐11b, CD‐14 (macrophage marker), CD‐16 [an antigen expressed by natural killer (NK) cells and granulocytes], and CD‐25. Also examined were a small number of other melanocytic lesions [two cases of lentigo maligna (Hutchinson's melanotic freckle) and five of intradermal naevi]. The results of the study document a population of cells with the morphological and immunophenotypic characteristics of NK cells in association with 10 of the 16 cases of CMM. These cells were consistently absent from the other melanocytic lesions studied. The presence of NK cells in association with some cases of CMM bears no clear relationship to the Breslow thickness, Clark level, tumour ulceration, or the presence of activated T cells as determined by expression of the CD‐25 antigen. Whilst an explanation for the significant numbers of NK cells in some CMM lesions is unclear, their presence in intimate association with tumour cells does prompt speculation regarding a possible role in determining the biological behaviour of the tumour. Additionally, the study has confirmed and extended previous findings with respect to the broad characterization of mononuclear cells present in the host infiltrate as

ISSN:0022-3417    

DOI:10.1002/path.1711610107

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

摘要:

AbstractThe differentiation between Spitz naevus and melanoma is at times difficult. The present study was undertaken to define means to positively identify such melanocytic tumours of doubtful malignancy. Immunohistochemical staining intensity for S100 protein and neurone specific enolase (NSE) was measured in sections of 35 Spitz naevi using a microcomputer image analysis system. The data were compared with results previously obtained from 19 cases of malignant melanoma and 16 benign compound naevi. Disaggregated cells from paraffin‐embedded material were stained by the Feulgen technique for DNA estimation. The nuclear DNA content distributions were measured using the same image analysis system. Compared with the malignant cases, the Spitz naevi showed significantly lower staining intensity for both S100 protein (P<0.0001) and NSE (P<0.0001). When compared with the benign compound naevi, the staining intensity was significantly lower for S100 protein (P=0.003). The nuclear DNA distribution in Spitz naevi proved to be a normal diploid pattern in 31 cases. Four cases showed a small proportion of hyperdiploid nuclei. The results show that Spitz naevi can be significantly distinguished from malignant melanoma by staining intensity for S100 protein and NSE. A normal diploid DNA content distribution appears to be typical for Spitz naevi. Spitz and benign compound naevi show dissimilar expression of S100 protein which may indicate different patterns of differentiation in these two types of lesion. The image analysis equipment used in this study is accurate, simple to use, produces results rapidly, and is economic. Therefore, it is clinically practicabl

ISSN:0022-3417    

DOI:10.1002/path.1711610108

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

摘要:

AbstractAll biopsies of membranous glomerulopathy collected between 1965 and 1988 (n=198) were examined for the presence of adhesions, focal sclerosis, protein crescents, capsular drops, and other capsular lesions. These were observed with a frequency related to the number of glomeruli in the biopsy and the number of sections examined. A peculiar interrelation between these lesions was found. In the 63 cases with sufficient clinical follow‐up, the capsular lesions were associated with a lower incidence of remission of the disease, and with more proteinuria and a higher serum creatinine at the last follow‐up. It is hypothesized that focal detachment of podocytes may result in the formation of a protein crescent if a plasma‐like filtrate is ‘injected’ between the epithelium of Bowman's capsule and its basement membrane. The detachment of the epithelium may lead to focal sclerosis and the formation of adhesions. The capsular drop is thought to be a final stage of inspissation of the protein

ISSN:0022-3417    

DOI:10.1002/path.1711610109

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

摘要:

AbstractQuantitative analysis of the mesangial matrix and cells was performed on serial renal biopsies from 41 children with IgA nephropathy. In the repeat renal biopsy, nine patients showed a significant increase of mesangial matrix, 29 showed no change and in three there was a significant decrease. Eight of the nine patients (89 per cent) with a matrix increase had persistent proteinuria at the second biopsy, whereas only 14 of the 32 (44 per cent) without a matrix increase had persistent proteinuria (P<0.05). Although the mesangial matrix increased in patients with persistent proteinuria, there was no decrease in patients with clinical remission. In contrast to the mesangial matrix, mesangial cells significantly decreased in 23 patients, did not change in 16, and significantly increased in only two in the second biopsy. These findings suggest that mesangial matrix increase is usually an irreversible change and that persistent proteinuria is associated with matrix increase with worsening in glomerular morphology and clinical outcome. This study indicates the importance of serial renal biopsy in children with IgA nephropathy with persistent proteinuria.

ISSN:0022-3417    

DOI:10.1002/path.1711610110

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

摘要:

AbstractEndoscopic biopsies of antral mucosa from 26 patients withHelicobacter pylori‐associated gastritis were studied by electron microscopy (EM). Scanning electron microscopy (SEM) showed clustering ofH. pyloriin the intercellular areas, being entrapped by the microvilli which were decreased at the sites where the bacilli were seen. The observations of SEM were confirmed by transmission electron microscopy (TEM), which showed adherence of the bacilli to the cell surface, producing cup‐shaped depressions in the epithelial cells, and occasionally intracellular infiltration byH. pylori.There were also depletion of mucus granules, degenerative changes, and disruption of intercellular junction complexes of the epithelial cells. Post‐treatment biopsies showed complete disappearance of the bacilli, and ultrastructural changes associated withH. pyloriinfection were res

ISSN:0022-3417    

DOI:10.1002/path.1711610111

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY