ISSN:0022-3417    

DOI:10.1002/path.1711720103

出版商:John Wiley&Sons, Ltd.    

年代:1994

数据来源: WILEY

摘要:

AbstractImmunohistochemical detection of intranuclear p53 gene product may indicate mutation of the p53 suppressor gene on chromosome 17p. We used six commercially available antibodies for p53 immunohistochemistry on 19 archival colorectal neoplasms and compared the results with the mutation status of the p53 gene and 17p allelic deletion status. By Friedman's ranking analysis, use of mouse monoclonal antibody DO7 with Target Unmasking Fluid (TUF) for antigen retrieval was the most sensitive and specific procedure (P30 per cent using a CAS 200 image analyser) had p53 mutation. Of seven tumours without expression (LI<1 per cent), six had no mutation and one had a truncating mutation which prohibited nuclear localization of gene product. The low expression group (1 per cent

ISSN:0022-3417    

DOI:10.1002/path.1711720104

出版商:John Wiley&Sons, Ltd.    

年代:1994

数据来源: WILEY

摘要:

AbstractImmunocytochemical detection of p53 protein products in paraffin sections is possible with a number of antisera, monoclonal and polyclonal. Few corroborative results amongst different laboratories have been published due to variations in the antibodies, the fixation protocols, and the immunocytochemical methods applied. Antigen unmasking methods employing microwaves or proteolytic enzymes add to the disparity in the percentage of positive cases reported. In this study, paraffin sections of 55 cases of cervical carcinoma were immunostained with monoclonal antibodies p53‐DO7 and p53‐1801 (a) without section pretreatment, (b) with pronase digestion, and (c) with microwave antigen retrieval in citric acid buffer. Specimens fixed in buffered formalin required antigen unmasking to show positive staining. Pronase digestion caused false‐negative immunostaining. Microwave pretreatment with p53‐DO7 yielded 100 per cent positivity for p53 proteins but only 7/55 cases with more than 50 per cent positive cells. Monoclonal antibody p53‐DO7 detected more positive cases than p53‐1801. Immunostaining with antibodies to p53 proteins must be interpreted cautiously as variations in fixation, antibodies, and section pretreatment will significantly aff

ISSN:0022-3417    

DOI:10.1002/path.1711720105

出版商:John Wiley&Sons, Ltd.    

年代:1994

数据来源: WILEY

摘要:

AbstractA new monoclonal antibody to human c‐junoncoprotein, designated NCL‐DK4, has been produced. NCL‐DK4 has been proved to be highly effective for use on formalin‐fixed, paraffin‐embedded tissues, enabling the study of c‐junexpression at a cellular level in both normal and neoplastic human tissues. The expression of c‐junoncogene has been examined in normal, benign, and malignant breast tissues, and c‐jun‐specific immunoreactivity in carcinomas has been related to histological type, tumour grade, c‐erbB‐2, oestrogen receptor, progesterone receptor, and epidermal growth factor receptor expression. Normal and benign breast tissues showed c‐jun‐specific immunostaining, which was weaker and in fewer cells compared with the c‐junimmunoreactivity observed in breast carcinomas. No relationship was found between the degree of immunostaining and the extent of proliferative changes in benign breast tissues. Ninety per cent of all breast carcinomas studied showed c‐jun‐specific nuclear staining. There were no statistically significant differences in the intensity of c‐junimmunoreactivity among grade I, II, and III infiltrating ductal carcinomas. There was no significant relationship between c‐junoncoprotein expression and c‐erbB‐2, oestrogen, progesterone, and epidermal

ISSN:0022-3417    

DOI:10.1002/path.1711720106

出版商:John Wiley&Sons, Ltd.    

年代:1994

数据来源: WILEY

摘要:

AbstractThe nm23 gene was originally identified by Steeget al.by screening of cDNA libraries from murine melanoma cell lines of varying metastatic potential. An inverse relationship between metastatic potential and nm23 RNA and/or protein was found in four different metastatic model systems. It was proposed that nm23 may function as a suppressor gene for tumour metastasis. It has recently been found that the sequence of nm23 and NDP‐kinase (NDP‐K) is identical. Using an immunohistochemical technique and employing a polyclonal antibody to purified NDP‐K A, we have determined NDP‐K expression in a series of 197 breast carcinomas. One hundred and sixty (81·2 per cent) of these tumours were scored positive for NDP‐K and 37 (18·8 per cent) scored negative. No relationship was found between NDP‐K/nm23 expression and patient relapse or survival. Furthermore, no relationship was found between NDP‐K/nm23 expression and a number of other prognostic factors including tumour grade, oestrogen receptor, progesterone receptor, and p53 expression. Our results contradict the hypothesis concerning the possible role of NDP‐K/nm23 as a metastatic suppressor gene in human breast cancer, but further studies using antibodies specific for NDP‐K/nm23 subtypes ar

ISSN:0022-3417    

DOI:10.1002/path.1711720107

出版商:John Wiley&Sons, Ltd.    

年代:1994

数据来源: WILEY

摘要:

AbstractThe clinicopathological and immunocytochemical features of nine cases of salivary duct carcinoma are described. This relatively rare tumour, which only recently has been widely recognized as a separate entity, is highly maligant and caused the death in eight of the patients. The tumour cells are arranged in cribriform and solid growth patterns, where the solid tumour nests frequently have comedo necrosis, and a fibrous, often sclerotic, stroma is present. The infiltrating desmoplasmic component and the diffuse invasive growth into adjacent adipose parotid tissue have similarities to ductal breast carcinoma. Immunocytochemical investigationof salivary duct carcinoma showed constant overexpression of c‐erbB‐2 as detected by membrane accentuation, and high proliferative activity as detected by nuclear positivity for MIB 1 (Ki‐67). Changes in the expression of p53 and retinoblastoma gene product do not constitute a constant event event in salivary duct carcinoma. A few of the tumours showed scattered cells with distinct nuclear positivity for both progesterone and oestrogen receptors. We emphasize that this highly malignant salivary gland tumour has a characteristic morphology, may not be as rare as previously considered, and that prompt and aggressive therapy is n

ISSN:0022-3417    

DOI:10.1002/path.1711720108

出版商:John Wiley&Sons, Ltd.    

年代:1994

数据来源: WILEY

摘要:

AbstractTwo monoclonal antiboides (MAbs) against human renal glomeruli, HEP1 and HBM1, were produced using isolated human glomeruli as an antigen. Immunohistochemical and immunoelectron microscopic analyses revealed that the recognition site of HEP1 is the cell surface of the visceral glomerularepithelial cells. HEP1 showed no reaction in the renal interstitium or in other tissues. HBM1 recognized an antigen present in the basal lamina of the renal glomeruli, Bowman's capsule, and partly in the basement membrane of renal tubules, but it was not reactive with the mesangial matrix or renal interstitium. The isotype of both MAbs was IgG1,k. The molecular weights of their corresponding antigens were 127–177 and 220kD, respectively. Besides human tissues, HEP1 also showed cross‐reactions with antigens in the visceral glomerular epithelial cells of various animals other than mice. HBM1 recognized human antigen only. Using both MAbs, eight cases of crescentic glomerulonephritis were examined immunohistochemically. As a result, it was demonstrated that the crescents may not be formed by visceral glomerular epithelial cells. From this study, these two MAbs appear to be userful markers for the evaluation of renal glomerular disord

ISSN:0022-3417    

DOI:10.1002/path.1711720109

出版商:John Wiley&Sons, Ltd.    

年代:1994

数据来源: WILEY

摘要:

AbstractWithin the group of primary cutaneous T‐cell lymphomas (CTCLs), mycosis fungoides (MF), Sézary's syndrome (SS), and CD30‐positive lymphomas have been delineated as clinicopathological entities. Primary CTCLs that do not belong to one of these entities represent a heterogeneous and ill‐defined group of neoplasms. This paper describes the clinical and histological features of 35 of such cases. The object of t his study was to define prognostic parameters for this group of primary CTCLs. Using a slightly modified version of the updated Kiel classification, a subdivision was made into CTCL, pleomorphic, small cell type (n= 3); plemorphic, medium‐sized cell type (n= 6); pleomorphic, large cell type (n= 18); and immunoblastic lymphomas (n= 8). Altogether, these lymphomas had a poor prognosis with estimated 2‐ and 4‐year survival rates of 53 and 22 per cent, respectively. Patients with pleomorphic, small and medium‐sized cell lymphomas (n= 9) proved to have a significantly better survival that those with pleomkorphic, large cell lymphomas (P= 0·032) and immunoblastic lymphomas (P= 0·008). Primary cutaneous immunoblastic lymphomas had the worst prognosis with an estimated 2‐year survival rate of 14 per cent. Other parameters including age (P= 0·345), sex (P= 0·345), sex (P= 0·662), extent of skin lesions at presentation (P= 0·0854), and mode of initial treatment (P= 0·609) had no significant effect on the survival time. The results of this study suggest that primary CTCLs other than classical MF, SS, and CD30‐positive lymphomas have a poor prognosis in most cases, and that the current classification may be a useful means of predicting the clinical beh

ISSN:0022-3417    

DOI:10.1002/path.1711720110

出版商:John Wiley&Sons, Ltd.    

年代:1994

数据来源: WILEY

摘要:

AbstractSuction blister injury is an experimental model for the investigation of the possible derangement of dermal/epidermal interaction in injury. An extensive fibre network can be stained in skin using antisera to the pan‐neuronal marker protein gene product 9.5 (PGP) and the sensory neuropeptide calcitonin gene‐related peptide (CGRP), while microvessels are identifiable with antisera to the endothelial marker von Willebrand's factor (vWf) and the peptide endothelin (ET). To investigate the possible involvement of superficial cutaneous innervation and microcirculation during the repair process in injury, human skin biopsies taken at differnt times after suction blister injury were investigated by quantitative immunohistochemistry. Neural and endothelial changes were seen in both edge and blister areas. PGP‐and CGRP‐immunoreactive nerves showed an increase in both areas compared with control skin up to 6 h after injury, followed by a decrease which lasted until 72 h. This was followed by a gradual increase of both nerve types starting from the blister edge and lasting up to 8 days after injury when the values were similar to controls. Similarly, in the blister area of the skin, vWF‐immunoreactive capillaries showed statistically significant increases between 0 and 6 h after injury, followed by a decrease at 12 and 18 h which was maintaine dup to 72h. ET‐1 immunoreactivity showed a similar, although more variable, pattern of changes. At the blister edge from 23 h onwards, both vWf and ET‐1 immunoreactivities showed a second increase from the edge of the blister spreading towards the centre of the blister ahead of the nerve increase. This lasted up to 8 days, when vWf immunoreactivity showed a statistically significant increase compared with control skin. Generally the numerical increase observed at early time points was accompanied by a strong staining intensity, which reverted to a normal staining intensity at later time points. These results suggest that there is a reactive process for all immunoreactive elements in the early stages of injury, followed by changes indicative of neuronal and endothelial damage (depletion phase) and the subsequent repair process. The repair started with re‐epidermalization from the blister edge, followed by revascularization and, lastly, by reinnervation of the tissue. These results indicate a close relationship between epidermis, blood vessels, and nerve fibres during the healing process which follows suction

ISSN:0022-3417    

DOI:10.1002/path.1711720111

出版商:John Wiley&Sons, Ltd.    

年代:1994

数据来源: WILEY

摘要:

AbstractAt endoscopy, the duodenum in Whipple's disease frequently appears abnormal and some clinical features such as gastrointestinal blood loss and anaemia suggest epithelial damage. However, the intestinal epithelial cells themselves appear to be normal on light and electron microscopy. The aims of this study were to analyse in detail the cytological changes in epithelial cells over time and in response to therapy in biopsies obtained from 20 patients, to investigate the functional repercussion on digestive enzymes such as lactase, and to assess the expression by the epithelial cells of MHC antigens. Cytological changes were minimal at both the light‐ and the electron‐microscopic level and MHC class I expression was preserved. However, changes indicative of functional deficits were demonstrated. Lactase and MHC class II expression were reduced or even absent. Antibiotic therapy resulted in normalization within 3–6 months. These findings are consistent with the clinical evolution and are of interest with regard to the importance of the immune response in aetiopathoge

ISSN:0022-3417    

DOI:10.1002/path.1711720112

出版商:John Wiley&Sons, Ltd.    

年代:1994

数据来源: WILEY