ISSN:0022-3417    

DOI:10.1002/path.1711580402

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractAnin situhybridization technique has been developed for assessing poly(A)+RNA preservation in routine pathology specimens. The method detects poly‐adenylated RNA sequences in tissue sections using a biotinylated polydeoxythymidine poly d(T)) probe. The probe was prepared from single‐stranded 25–30 base oligo d(T) and was biotinylated using the enzyme terminal deoxynucleotide (ransferase with biotin‐11‐dUTP and dTTP in the ratio 1:4. The hybridization protocol uses varying concentrations of proteinase K to unmask mRNA sequences and the biotin labelled hybrids are demonstrated after hybridization under standard conditions by the application of streptavidin and biotinylated alkaline phosphatase. Alkaline phosphatase was visualized using a Fast Red naphthol‐capture method and the sections were counterstained with haematoxylin.The results have confirmed that the method is specific for poly(A)+RNA and shows that poly(A)+RNA can be demonstrated in routine formalin‐fixed sections using non‐radioactive techniques with retention of morphology. It also provides a means of optimizing the hybritdization conditions for specific mRNA probes and produces a staining pattern demonstrating the relative level of poly(A)+RNA per cell which may reveal new information about cell activity and

ISSN:0022-3417    

DOI:10.1002/path.1711580403

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractTo elucidate the synthetic activity in relation to the morphology of the epithelial cells of the small and large intestine,in situhybridization with a poly‐deoxyribothymidine (poly d(T)) probe was applied to paraffin sections of formalin fixed blocks. This effectively displays poly‐adenylated RNA and, by implication, messenger RNA (mRNA). By minimizing proteinase K pretreatment, the relative concentrations of cellular mRNA were visualized. This revealed minimal mRNA in crypt columnar cells, and maximal mRNA in proliferating cells and in cells showing terminal differentiation. The latter include surface epithelial cells, endocrine cells, Paneth cells, and maturing, but not mature, goblet cells. The goblet cells showing positive hybridization can be regarded as active cells and show characteristic morphology. Such cells are particularly evident in untreated coeliac disease, remitting ulcerative colitis, and transitional mucosa. The proliferating cells showing increased hybridization include normal mitotically active crypt epithelium, regenerating epithelium in ulcerative colitis, adenomatous epithelium, and adenocarcinomatous epithelium. The similarity of hybridization between metaplastic polyp epithelium and surface colonocytes is consistent with the concept that metaplastic polyps are formed of cells showing premature terminal differentiat

ISSN:0022-3417    

DOI:10.1002/path.1711580404

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractParathyroid hormone related protein (PTHrP) has been demonstrated in the tumour cells of squamous cell carcinomas originating in a variety of organs, in undifferentiated small cell tumours of the bronchus, and in carcinoma of the kidney. The protein hormone is thought to produce the hypercalcaemia which may complicate some of these malignan cies. By using an antibody raised in rabbits against the N‐terminal portion of the molecule, the polypeptide may be demonstrated in cells of the prickle cell layer of normal skin and in the cells of hair follicles. Skin tumours showing squamous or hair follicle differentiation are shown to contain the protein antigen, while basal cell carcinomas and tumours with sweat gland differentiation do not The hormone may be primordial in origin and the progenitor of parathyroid hormon

ISSN:0022-3417    

DOI:10.1002/path.1711580405

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractA novel method for grading intestinal metaplasia, with general applicability, is described. Highly significant agreement was found by kappa analysis both for intra‐observer (k=0.90) and for inter‐observer (k=0.85) variation, confirming the reproducibility of the method. The ranks derived by this method were correlated with gastric tissue levels of the enzymes beta‐glucuronidase, lactic dehydrogenase (LDH), and %m‐LDH. A significant (P<0.05) positive correlation was found between intestinal metaplasia and %m‐LDH, but there was no correlation between intestinal metaplasia and beta‐glucuroni

ISSN:0022-3417    

DOI:10.1002/path.1711580406

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractThe purpose of this study was the quantitative assessment of mucosal inflammation and its relationship toCampylobacter pyloriin gastric antral and body biopsies from patients with dyspepsia and controls. The study groups comprised patients with duodenal ulcer (DU;n= 20), duodenitis (DUN;n= 20), non‐ulcer dyspepsia (NUD;n= 20), Using a semiautomatic, computer‐linked image analyser (Kontron: MOP Videoplan), mucosal acute and chronic inflammatory cell densities were measured in defined gastric sites for each patient group and expressed as number per mm2of lamina propria and number per mm length of epithelium. Measurements were also made on a group of asymptomatic controls (n= 9) who fulfilled strict exclusion criteria. All biopsies were analysed for the presence ofCamplyobacter pylori(CP) with a Giemsa stain. Data between groups were compared using the Mann‐WhitneyU‐test. In the antrum and body, the mononuclear cell count was significantly higher in lamina propria in DU patients than in DUN, NUD and controls. In the body, DU laminia propria mononuclear cell counts were higher than those of DUN and controls. Prevalence rates for CP for DU, DUN, and NUD were 94, 89, and 50 per cent for antral and 88, 83, and 56 per cent for body biopsies. Significant differences were present between CP‐positive and negative subjects in the NUD group. Antral and body inflammation within these clinical groups shows a wide variation. Higher inflammatory cell counts in the DU group may reflect the prevalence of CP col

ISSN:0022-3417    

DOI:10.1002/path.1711580407

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractUsing an immunohistochemical technique, the development of the cytoskeletal proteins desmin, vimentin, and actin (using alpha isotype and non‐isotype specific antibodies) was assessed using a semi‐quantitative grading system in the pulmonary vascular smooth muscle of nine normal pigs and 19 normal humans at different ages, and in 13 children with pulmonary hypertensive congenital heart disease. In the normal of both species, immunostaining for vimentin decreased after birth and then increased gradually while immunostaining for desmin and alpha actin increased steadily with age. In pulmonary hypertension, immunostaining for alpha actin and vimentin showed an accelerated increase at between 2 and 8 months. Also, the media showed regional differences in immunostaining which preceded the development of intimal proliferation. The inner media showed less immunoreactivity for all cytoskeletal proteins studied than did the outer media. Within areas of intimal proliferation many cells were immunonegative. These results suggest that the cytoskeletal features of medial smooth muscle cells are remodelled in the normal infant; that this process is altered from at least 2 months in the pulmonary hypertensive infant; and that the smooth muscle cells immediately beneath the internal elastic lamina are remodelled before migrating to form intimal proliferation. Changes in cytoskeletal composition can be related to the previously described postnatal maturation of pulmonary vascular smooth muscle ce

ISSN:0022-3417    

DOI:10.1002/path.1711580408

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractWe have identified several antibodies which in immunocytochemical experiments reveal antigenic heterogeneity in renal vascular endothelium. In the renal vascular endothelium, blood group antigen H type II was carried in a sialylated form on an endogalactosidase resistant molecule. The glomerular reactivity was sensitive to digestion by this enzyme. Monoclonal antibodies of the CD 39, CD 14, and CD 11C leucocyte differentiation clusters bind to renal vascular endothelium but not to glomerular capillaries. In fetal kidney, the developing glomerular endothelium has the same phenotype as the remainder of the vascular tree with respect to these markers. There are therefore antigenic differences among renal endothelial cells and these arise during renal organogenesis.

ISSN:0022-3417    

DOI:10.1002/path.1711580409

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractAnti‐glomerular basement membrane antibody‐induced glomerulonephritis (anti‐GBM nephritis) was transferred from nephritic rats to normal recipient rats with isologous antibodies obtained from the urine of the nephritic rats. The original actively‐immunized anti‐GBM nephritis was induced in inbred WKY/NCrj rats by injecting the nephritogenic antigen from bovine renal basement membranes. Excreted urinary antibodies were collected, purified, and then injected into recipient rats of the same strain, Haematuria and proteinuria appeared on day 2 and day 3, respectively; both became heavier and reached a plateau by day 5. Endocapillary hypercellularity of mononuclear cells in glomeruli was the first histological change, which was observed from day 2, and later extracapillary changes such as fibrin deposition, capsular adhesion, and crescent formation were observed. These histological changes were the same as those seen in the actively‐immunized nephritis. The results demonstrate that anti‐GBM nephritis is clearly induced by autoantibodies. This new passively‐immunized model of anti‐GBM nephritis makes it possible and easier to analyse further the mechanism of anti‐GBM nephritis because only isologous antibodies are used. This study also indicates that the urine of the nephritic rat is a good sourc

ISSN:0022-3417    

DOI:10.1002/path.1711580410

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711580411

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY