ISSN:0022-3417    

DOI:10.1002/path.1711640102

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractWe have used immunocytochemistry to identify the major primary adhesion molecule of the cadherin class in human kidney. In frozen sections of kidney, A‐CAM was detected using the monoclonal antibody GC 4 on the surface of renal tubular epithelial cells. Renal tubular epithelium did not express L‐CAM. No cadherin reactivity was found on the glomerular epithelial cells. Cultured renal tubular epithelium was studied by immunofluorescence and immunogold methods. A‐CAM was found at the contact points of adjacent epithelial cells, the phenotype of which was confirmed by the demonstration of cytokeratins using the antibody CAM 5.2. The A‐CAM molecule in human kidney had anMrof 130 kD in Western blotting experiments. These results lead us to conclude that A‐CAM is the major cadherin of adult human renal e

ISSN:0022-3417    

DOI:10.1002/path.1711640103

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractWe have used fluorescentin situhybridization and simultaneousin vivobromodeoxyuridine labelling of a solid bladder cancer to examine tumour cell subsets for possible proliferative growth differences. In this dual‐labelled preparation, most tumour cell nuclei exhibited monosomy 9, consistent with reported karyotypes of bladder cancer.1Incorporated bromodeoxyuridine was visualized with a fluoresceinated antibody in 5–6 per cent of the tumour cells, concordant with S‐phase estimates by cell cycle analysis of the flow cytometric DNA histogram. A majority of the bromodeoxyuridine‐positive cells also carried the monosomy 9 chromosome abnormality. This is the first report to demonstrate the feasibility of combinedin situhybridization and detection of bromodeoxyuridine incorporatedin vivoin human tumour cells in order to provide information on the growth rate of specific subsets of tumour cells identified by chromosomal const

ISSN:0022-3417    

DOI:10.1002/path.1711640104

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractThe nature and nosologic status of malignant fibrous histiocytoma (MFH), or at least its most common pleomorphic variant, are a matter of controversy and it is possible that this entity represents the shared morphologic phenotype of a range of other dedifferetiated neoplasms. An unusual case of malignant peripheral nerve sheath tumour which very closely mimicked pleomorphic MFH is presented. Its true nature was only disclosed by ultrastructural examination. A further interesting and previously unreported feature in tumours of this type was the presence of annulate lamellae in many of the tumour cells. This case adds weight to the assertion that the light microscopic features of pleomorphic MFH may not be specific.

ISSN:0022-3417    

DOI:10.1002/path.1711640105

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractWe describe eight patients who developed interstitial pulmonary fibrosis following BCNU (carmustine) therapy for cerebral tumours. The fibrosis presented 12–17 (mean 14) years after exposure to the drug. A distinctive pattern of pulmonary fibrosis with involvement of the apices and subpleural areas was seen in one patient dying of the disease. Light microscopy showed interstitial elastosis and intra‐alveolar fibrosis which was often focal with an associated mild lymphoplasmacytic infiltrate, intra‐alveolar oedema, macrophages, and some neutrophils. Ultrastructural studies showed electron lucency of type I pneumocytes, with breaks in the cytoplasmic membranes leaving a bare basement membrane. Degenerative change was also seen in endothelial cell cytoplasm along with lipofuscin deposition.While BCNU pulmonary fibrosis has been described up to 2 years after treatment, this complication so late after therapy, though rare, has important implications for the follow‐up of patients receiving th

ISSN:0022-3417    

DOI:10.1002/path.1711640106

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractIn situhybridization techniques were used to detect immunoglobulin light chain messenger RNA (mRNA) in 28 formalin‐fixed, paraffin‐embedded samples of Hodgkin's disease. Cocktails of biotinylated oligonucleotide probes specific for the constant regions of kappa and lambda light chain mRNA were used. None of the Reed‐Sternberg cells or their variants in any of the cases studied showed positive staining with either probe, in contrast to normal plasma cells which showed strong staining in the same sections. It was concluded, therefore, that the cytoplasmic immunoglobulin frequently detected within these cells by immunocytochemistry is present not as a result of synthesis, but as a result of some other mech

ISSN:0022-3417    

DOI:10.1002/path.1711640107

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractMice inoculated withMycobacterium tuberculosis, strain H37Rv were used as a model of human tuberculosis. The microanatomical location of immunoperoxidase staining with a polyclonal anti‐BCG serum was within macrophages and appeared granular rather than delineating whole bacilli. Immunoperoxidase staining appears to demonstrate degraded mycobacterial antigens from disrupted organisms and so reflects prior turnover of bacilli. On Ziehl–Neelsen staining, intact or almost intact bacilli are seen and so the extent of this form of staining reflects the current bacillary load. Both methods have limited sensitivity, but with larger mycobacterial loads the area of immunoperoxidase stain measured on a semi‐automated image analyser correlated with the numbers of bacilli observed. The immunoperoxidase method will be useful in the evaluation of residual antigen in studying the pathogenesis of experimental murine tuberculosis. In human mycobacterial granulomata, this immunohistochemical technique should provide an alternative method of estimating the extent of bacillary load: this approach may also provide evidence of mycobacterial infection from residual antigen deposits in the tissue when whole bacilli have been successfully cl

ISSN:0022-3417    

DOI:10.1002/path.1711640108

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractA recent report describing the distribution of L1 epithelial antigen in lung tumours in relation to the histological type claimed that this antigen was a highly reliable marker of squamous cell carcinoma. Our study was designed to test this claim and to examine the potential of this antigen in the typing of lung tumours in biopsy specimens. A total of 143 lung tumours were typed according to the WHO classification and examined immunhistochemically for L1 epithelial antigen experession using commercially available monoclonal mouse anti‐human myeloid/histiocyte antigen (MAC 387). Positivity was found in 46 of 55 squamous cell carcinomas (84 per cent), 12 of 27 adenocarcinomas (44 per cent), 15 of 16 adenosquamous carcinomas (93 per cent), 10 of 15 large cell carcinomas (67 per cent), none of 20 small cell carcinomas, and none of 10 carcinoid tumours. Of those tumours expressing L1 epithelial antigen, most showed a patchy pattern of positivity. From this study it is clear that detection of L1 epithelial antigen, most showed a patchy pattern of positivity. From this study it is clear that detection of L1 epithelial antigen by MAC 387 antibody is not specific for squamous cell carcinomas, but it may have a limited use in the diagnosis of small cell carcinomas and carcinoid tumours as these are consistently negativ

ISSN:0022-3417    

DOI:10.1002/path.1711640109

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractThis study was undertaken to assess a post‐hybridization amplification system for increasing the sensitivity ofin‐situhybridization with oligodeoxyribonucleotide (oligonucleotide) probes.The technique employs a multilayer streptavidin–biotinylated link protein amplification technique, similar to that used in the ABV histochemical amplification system, to attach increasing numbers of radiolabelled streptavidin molecules to a biotinylated ologonucleotide probe.In tissue sections the amplification technique increases the signal‐to‐noise ratio dramatically, increasing the sensitivity ofin situhybridization and reducing the autoradiographic exposure time. On an artificial medium the amplification technique has been shown to increase the hybridization signal 100‐fold when compared with a directly radiolabelled oligonucleotide probe. The technique could be used to compare relative amounts of target molecule, there being a linear relationship between the detectable signal and the log of the concentration of the targ

ISSN:0022-3417    

DOI:10.1002/path.1711640110

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY

摘要:

AbstractThe rectal adenocarcinoma cell line, HRA‐19a1.1, was cultured in a three‐dimensional type I collagen gel and then xenografted in nu/nu mice to determine whether thein vivoenvironment could induce further morphological differentiation of thein vitrocollagen gel cultures. Three phenotypic changes were observed. Type I collagen induces glandular differentiationin vitroin which well polarized cells are organized around a central lumen, as has been previously reported.1Seven days after xenografting this structure in nu/nu mice, the glandular structures appeared to have ‘ballooned’ forming cyst‐like structures lined by a monolayer of flattened cells. There were no stromal cells associated with the graft at this stage, but with time stromal cells invaded the collagen. At points where these cells were closely associated with the HRA‐19 cells there was a marked phenotypic change, with the flattened lining cells seen at day 7 becoming columnar. By 21 days the stromal cells had replaced the collagen and the histology of the graft now resembled that of an adenocarcinoma. Placing this cell–collagen culture in a Millipore chamber prior to grafting resulted in cyst‐like structures only. Here we provide conclusive evidence that heterologous connective tissue cells can induce differentiation of a rectal adenocarcinoma cell line by a non‐ or poorly diffusiblc factors(s). Furthermore, we show that this cell–collagen xenograft method has certain advantages over conventional xenograft methods: notably, a consistent 100 per cent take rate; considerably fewer cells are required to form a tumour; and the time taken to form a tumour is d

ISSN:0022-3417    

DOI:10.1002/path.1711640111

出版商:John Wiley&Sons, Ltd.    

年代:1991

数据来源: WILEY