ISSN:0022-3417    

DOI:10.1002/path.1711710402

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711710403

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711710404

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractThe integrins are αβ heterodimeric transmembrane proteins mediating cell‐substratum as well as cell‐cell interactions. Previous distribution studies on integrin expression have been limited by the requirement for cryostat sectioned tissues, and consequent poor resolution. We have examined 40 examples of ductal carcinomain situ(DCIS) for the expression of both β1 and β4 integrin chains. These showed strong polarized membrane staining of residual myoepithelial cells (correlating with expression of smooth muscle specific actin) and of the basement membrane region with β1 and β4 antibodies respectively. In 12 out of 40 cases, the DCIS was negative for the β1 chain and a variable pattern of reactivity was seen in the remaining cases. The β4 chain was detected focally and weakly in the tumour cells of 7/40 DCIS and strongly in one; all of these cases were also positive for the β1 chain. Of the 22 cases where co‐existent invasion was present, the infiltrating component showed either a similar degree or a diminution of the extent of immunostaining when compared with thein situcomponent; only one showed enhanced staining (β1 only). This study demonstrates that two of the main β chains, β1 and β4, can be effectively demonstrated on methacarn and cold (4°C) formalin‐fixed tissues by avidin‐biotin indirect immunoperoxidase staining and that the results are similar to those achie

ISSN:0022-3417    

DOI:10.1002/path.1711710405

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractThe presence of pS2 protein (pS2) was studied in a total of 120 consecutive patients with gastric carcinomas. This immunohistochemical study found pS2 expression in 48 per cent (n=58) of carcinomas. pS2 expression was also detected in normal gastric mucosa in 95 per cent (n=102) of specimens in upper antral mucopeptic glands and deep foveolar cells of the gastric pits but not in intestinal metaplasia. There was a significant statistical correlation between pS2 expression and extent of tumour growth (pT state) and expression of pepsinogen II by the tumours. There was no statistical correlation with clinical features such as patient age or sex or other pathological parameters (tumour stage, size, grade, and localization or growth pattern according to histological classification). There were no statistically significant differences in survival times between patients with pS2‐positive and pS2‐negative tumours. In contrast to findings concerning breast cancer, pS2 expression in gastric carcinomas has no influence on the patient's prognosis. On the other hand, strong expression of pS2 by surface epithelium of normal gastric mucosa may indicate that pS2 might play a role in physiological cell renewal of normal gastric muc

ISSN:0022-3417    

DOI:10.1002/path.1711710406

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractThere is considerable evidence suggesting that respiratory viral infection is involved in the genesis of the sudden infant death syndrome (SIDS), with rates of about 20 per cent of SIDS victims compared to about 13 per cent of controls. Since the techniques used previously are prone to under‐reporting from autopsy material, non‐isotopicin situhybridization (NISH) has been used to detect viral nucleic acid in lung in SIDS. Forty‐five SIDS cases (30 males) were examined (age range 3 weeks–14 months, mean age 3·9 months). Thirty non‐SIDS cases (15 males) were also examined (age range 5 weeks–24 months, mean age 9·0 months). Eleven of 45 (24·4 per cent) SIDS cases were positive by NISH compared to 1 of 30 (3·3 per cent) non‐SIDS cases (P=0·012). There were eight cases of adenovirus type 5, two cases of respiratory syncytial virus (RSV), and one case of parainfluenza virus type 2. The one positive control case was adenovirus type 5. Only lung parenchyma was examined here. Additional examination of the upper respiratory tract may increase the number

ISSN:0022-3417    

DOI:10.1002/path.1711710407

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractNuclear organizer regions (Ag‐NORs) were visualized using the standard silver‐staining technique in biopsy specimens from 112 bladder carcinomas. The area of individual Ag‐NOR dots and the area of Ag‐NOR protein/nucleus were measured using a Quantimet 570 image analyser and the results were then correlated with clinical, histological, and quantitative prognostic factors, and survival. The mean area of Ag‐NOR dots was not significantly related to any of the features analysed. The mean area of Ag‐NOR protein/nucleus was related to WHO grade (P=0.0004), papillary status (P=0.004), DNA ploidy (P=0.02), S‐phase fraction (P=0.02), mitotic frequency/mm2of enoplastic epithelium (P=0.005), mean nuclear area (P=0.001), and SD of nuclear area (P=0.001). In the entire cohort, the mean area of Ag‐NOR protein/nucleus was related to progression with a borderline significance (P=0.09), while in survival analysis only Ag‐NOR count/nucleus had prognostic value (P=0.010). In Ta‐T1 tumours, Ag‐NOR protein area/nucleus predicted progression (T‐category) (P=0.016) and survival (P=0.014) significantly. Also, the mean area of individual Ag‐NOR dots (P=0.026) and Ag‐NOR count/nucleus (P=0.014) were related to

ISSN:0022-3417    

DOI:10.1002/path.1711710408

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractThe expression of the nuclear protein p53 in oligodendrogliomas was investigated by immunohistochemistry, using a monoclonal anti‐p53 antibody (DO‐7) on formalin‐fixed, paraffin‐embedded material in 84 histologically verified cases, and compared with the histopathological grade and survival. p53‐immunoreactive cells were found in 75 per cent of the samples acquired at the first biopsy. The p53 labelling index was not related to the degree of nuclear anaplasia. Tumour cases with more than 75 per cent p53 immunostained cells had a rapidly fatal clinical course. However, no significant correlation was found between p53 labelling index and tumour grade, mitotic index, or ploidy status. In most tumour recurrences (n=25), the p53 labelling index increased or remained at the level of the first biopsy. In five cases (6 per cent), p53 was absent in the first sample as well as in the recurrence. Irrespective of the underlying aberration of either the gene or the metabolic pathway of p53, it is concluded that a high percentage (i.e., more than 75 per cent) of p53‐immunolabelled cells is predictive of an unfavourable clinical course, while a percentage lower than 75 per cent immunoreactive cells does not exclude a rapid fa

ISSN:0022-3417    

DOI:10.1002/path.1711710409

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractThe customary wave of hepatocyte regeneration which occurs in the rat liver after two‐thirds partial hepatectomy can be abolished by oral administration of the carcinogen 2‐acetylaminofluorene. Instead, regeneration is achieved through the proliferation and differentiation of potential stem cells (oval cells) which appear to emanate from the portal space. Ultrastructural studies have illustrated the undifferentiated nature of these cells in the first 3 days after resection, but very rapidly they acquire features of small hepatocytes or biliary epithelia. Oval cell progeny can form either cohesive columns of cells within sinusoids which may later differentiate into new hepatic plates, or single cells that can insinuate within existing plates. Using a35S antisense riboprobe to hepatocyte growth factor (HGF) mRNA, the synthesis of HGF mRNA was observed in sinusoid‐lining cells. There were few HGF mRNA‐expressing cells in the liver removed at resection, but numbers steadily increased in the remnant over the next 7 days. In particular, an almost nine‐fold increase in the density of HGF mRNA‐producing cells occurred in the periportal areas, resulting in approximately double the density present within the centrilobular parenchyma. The superabundance of HGF‐producing cells in the immediate vicinity of oval cell proliferation and differentiation strongly suggests that this growth factor is involved in all aspects of stem cell behaviour—proliferation, migration, and differentiation, through a para

ISSN:0022-3417    

DOI:10.1002/path.1711710410

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY

摘要:

AbstractThe occurence of apoptosis and its contribution to growth‐phase transitions in the rat ascites hepatoma Yoshida AH‐130 have been evaluated. Apoptosis was observed by light microscopy in both exponentially‐growing and stationary tumours as characteristic chromatin condensation and compacting of the cytoplasm, but the frequency of apoptotic bodies (apoptotic index) was four‐fold higher in stationary than in logarithmic‐growing tumours. Apoptosing cells exhibited strong immunocytochemical reactivity for ‘tissue’ transglutaminase, and transglutaminase activity was higher in stationary tumour cells. A ladder pattern of DNA fragmentation was revealed by agarose gel electrophoresis which was more pronounced in stationary tumours. The apoptotic bodies were either free or contained in vacuoles within otherwise normal tumour cells, suggesting active engulfment of dead cells by viable homologous cells. Such ‘homophagic’ disposal of the apoptotic bodies probably accounted for a component of the enhanced cell protein degradation previously observed in stationary AH‐130 tumours (Tessitore L, Bonelli G, Cecchini G, Amenta JS, Baccino FM,Arch Biochem Biophys1987;255:372–384). The data indicate that, in concurrence with changes in the cell proliferation rate, modulations of apoptosis play a substantial role in determining the net growth rate of such an anaplastic tumour as the Yo

ISSN:0022-3417    

DOI:10.1002/path.1711710411

出版商:John Wiley&Sons, Ltd.    

年代:1993

数据来源: WILEY