摘要:

AbstractA wide range of immunoreactive antigens commonly investigated in diagnostic pathology were evaluated in tissues subjected to fixation by microwave irradiation in normal saline to 63°C. All of the 23 antigens studied were well preserved and compared with corresponding tissues conventionally fixed by formaldehyde for periods of 16 and 18 h, respectively, immunostaining in microwave‐irradiated tissues was clearly superior, being more intense and also more extensive. Proteolytic digestion was not necessary for irradiated tissues except for the staining of cytokeratins and desmin. It was also possible to stain for Leu‐14 (CD22) and Leu‐1 (CD5), whereas these lymphocyte antigens were lost during formaldehyde fixation. Cytomorphological details were well preserved in the rapidly‐fixed irradiated

ISSN:0022-3417    

DOI:10.1002/path.1711560402

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractThe expression of keratins, CEA, EMA, and rat liver antigen (RLA) and the presence of Ki67+proliferating cells were studied in the epithelial linings of 50 odontogenic cysts using an indirect immunoperoxidase method on acetone‐fixed frozen sections. All cysts were positive with monoclonal antibodies of broad keratin specificty (CK1, AE1‐3), and between 40 and 100 per cent of epithelial cells expressed keratins 13 and 19. Keratins 7, 8, and 18 were rarely expressed although surface cells in areas of mucous metaplasia often expressed keratins 7 and 18. Expression of keratin 10/11 was related to the presence of a well‐ordered epithelial lining and was detected in isolated cells in 4/32 non‐keratinizing cysts and in the upper suprabasal cell layers of 17/18 keratocysts. Although CEA, EMA, and RLA were detected in the epithelium of all specimens, the pattern of expression of CEA and EMA differed between cyst types. Ki67+proliferating cells were most prevalent in keratocyst epithelia, where they were usually found within lower suprabasal layers which were negative or weakly positive for keratins 10/11 and 13. These results indicate differences in keratin, CEA, and EMA expression between cyst types which appear to be dependent on epithelial differentiation/structure rather than cyst type or histogenesis. Although these differences may not be of diagnostic significance, the consistent expression of both keratins 13 and 19 may provide a useful marker of odontogenic epithelium in

ISSN:0022-3417    

DOI:10.1002/path.1711560403

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractThe renal biopsies of ten women with preeclampsia without other underlying renal disease were examined in detail using light and electron microscopy and immunofluorescence. Characteristic preeclamptic glomerular lesions with endocapillary cell swelling, subendothelial and mesangial deposits, and mesangial interposition were detected in each patient. Juxtaglomerular regions were not prominent and were poorly granulated on light microscopy; ultra‐structurally, they showed myoepithelioid cells with sparse renin granulation and considerable heterogeneity of granule size and density in association with relatively meagre granular endoplasmic reticulum and Golgi profiles. These morphologic findings suggest that, in patients with clinical and renal biopsy evidence of preeclampsia, there is no significant stimulation of the renin–angiotensin sys

ISSN:0022-3417    

DOI:10.1002/path.1711560404

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractAn antiserum to carcinoembryonic antigen (CEA) and a monoclonal antibody to cytokeratin 19 (CK 19) were studied for their suitability as diagnostic reagents for the differential diagnosis of primary and secondary malignant epithelial tumours of the liver, on paraffin sections. With the antiserum to CEA, positive bile canalicular structures were found in 60 per cent of the hepatocellular carcinomas. All the cholangiocarcinomas and 66·6 per cent of the metastatic carcinomas were positive for CEA, without displaying a canalicular staining pattern. All the hepatocellular carcinomas were negative for CK 19. All the cholangiocellular carcinomas and the metastatic carcinomas were positive for CK 19. This staining profile may prove helpful in difficult diagnostic cases

ISSN:0022-3417    

DOI:10.1002/path.1711560405

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711560406

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractWe studied by microscopy 377 biopsies, nephrectomies, and necropsy kidneys from 123 human renal transplants. We discovered two common abnormalities of the renal corpuscle, both affecting the glomerulo‐tubular junction. Adhesion of the tip of the glomerular tuft to the origin of the tubule, as reported in various non‐transplant glomerulopathies, was seen in 197 specimens (52 per cent). This change was common in material showing acute or chronic vascular rejection and glomerulopathy, and was almost universal in transplants that had been in place for over 1 year. Another change at the glomerulo‐tubular junction, not previously highlighted, consisted of an infiltrate of lymphocytes or neutrophil polymorphs into the epithelium at the tubular origin. This change was seen in 145 specimens (38 per cent) and was associated with cellular rejection and ascending infection. These changes are of importance because they show two responses of the kidney to injury that involve the glomerulo‐tubular junction and thus suggest that this part of the kidney has some specific properties that have been largely neglected up

ISSN:0022-3417    

DOI:10.1002/path.1711560407

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractThe monoclonal antibody PAL‐E is specific for endothelial cells in a wide variety of normal and tumour tissue. In normal kidney, PAL‐E reacts exclusively with the endothelium of non‐glomerular blood vessels. In Wilms' tumour, binding of PAL‐E was not restricted to the endothelium; staining of blastemal cells was observed in seven out of eight cases examined. Mesenchymal and tubular components, if present in Wilms' tumour, were negative. In contrast, a monoclonal antibody to Factor VIII‐related antigen (RFF‐8‐R‐1) bound only to endothelial cells in these tumours. In fetal kidney, PAL‐E binding showed a wider distribution than in adult kidney and both stromal and glomerular capillaries were stained. Tubules and non‐endothelial stromal cells were negative. These results indicate that the reactivity of the monoclonal antibody, PAL‐E, is not restricted to cells of endothelial origin in all tissues. The implications of these findings for the differentiation of Wilms'

ISSN:0022-3417    

DOI:10.1002/path.1711560408

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractAs endocrine tumours in a number of organs may arise in a background of hyperplasia, the density of endocrine cells in appendices from ten patients with carcinoid tumours was compared with that in appendices from ten age‐ and sex‐matched control patients. Crypt and lamina propria endocrine cells were quantified separately.The density of argentaffin endocrine cells in the crypts was significantly higher in appendices with carcinoid tumours when compared with the controls. No difference was found in non‐argentaffin endocrine cells, and no difference was found in either argentaffin or argyrophil endocrine cells in the lamina propria. While it is possible that carcinoid tumours induce an increase in the number of enterochromaffin (EC) cells in the background mucosa, it is considered more likely that EC cell hyperplasia predisposes to the development of carcinoid tumours of the app

ISSN:0022-3417    

DOI:10.1002/path.1711560409

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractForty‐four SJL mice were killed at intervals to follow the spontaneous development of lymphomas. These were detected in 28 of 38 mice aged 24 weeks or more. Immunoperoxidase stains were applied on cryostat sections of lymphomas from various sites (spleen, mesenteric lymph nodes, Peyer's patches, and other lymph nodes), sampled from 19 mice of different age groups, to study tumour immunophenotype in correlation with histological features. With a panel of antibodies against Thy 1·2, Lyt 1, and Lyt 2, and the mouse immunoglobulin heavy and light chains, all the 19 tumours were shown to be B‐cell derived. This was further supported by the detection of Ia antigens on the tumour cells. Six mice had plasmacytoid or predominantly plasmacytoid tumours expressing γκ. Three mice had mixed plasmacytoid and pleomorphic tumours which also showed γκ positivity. The ages of these nine mice ranged from 24 to 44 weeks with a median of 36 weeks. Pleomorphic tumours were found in ten mice (age range = 36–52 weeks, median = 44 weeks). These tumours were more heterogeneous, expressing either α, μ, or γ, or with loss of heavy chain expression altogether. These age‐related changes are probably a sign of progressive loss of differentiation

ISSN:0022-3417    

DOI:10.1002/path.1711560410

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractSulindac, a non‐steroidal anti‐inflammatory drug, has been reported to lead to tumour regression in cases of human polyposis coli. We have investigated the effects of this drug on the growth of 1,2‐dimethylhydrazine (DMH)‐induced mouse colonic tumours. In one experiment, DMH and oral sulindac were administered concurrently to a group of mice for a period of up to 24 weeks, while a control group of animals received DMH only for the same period. Sulindac caused a significant reduction in both the number of mice with colonic tumours and the number of tumours per mouse. In a second experiment, two groups of mice which had already been treated with DMH for 17 weeks received either sulindac or not for 78 days. In this experiment sulindac had no effect. These results demonstrate that sulindac has a protective effect against the chemical induction of colonic tumours in mice, but does not cause the regression of established

ISSN:0022-3417    

DOI:10.1002/path.1711560411

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY