摘要:

AbstractThe growth of neoplasms originating from steroid hormone target tissues may be hormone‐dependent. It has been clearly demonstrated that the oestrogen and/or progestagen receptor status predicts the response to endocrine treatment and the prognosis of breast cancer. However steroid receptor determination in tumour cytosols has several limitations, which can partly be resolved by (immuno) histochemical detection of steroid hormone‐responsive tumour cells. A variety of histochemical techniques including autoradiography of tritiated steroids, immunohistochemistry with antibodies to steroid hormones, histochemistry with fluorescent ligands of low molecular weight and fluorochrome‐ or enzyme‐labelled macromolecular probes are discussed. It is concluded that there is as yet no substantial evidence that these methods visualize steroid receptors or detect steroid hormone‐responsiveness of tumour cells. On the other hand, immunohistochemical demonstration of oestrogen receptors with monospecific antibodies seems validated and indications have been obtained that this information is clinically relevant. Recent developments suggest that immunocytochemistry of receptors and specific hormone‐induced proteins, flow cytometric analysis and probably hybridocytochemistry in the future will help to improve management of steroid hormone‐depe

ISSN:0022-3417    

DOI:10.1002/path.1711490302

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

摘要:

AbstractThe utilization of [3H] sugars and leucine by non‐malignant and malignant human breast has been assessed using an organ culture technique with subsequent tissue autoradiography. The uptake of sugars by normal and hyperplastic breast was generally constant, with some differences observed in the utilization of galactose by acini of normal and hyperplastic tissues. After 24 h incubation localization was predominantly at the luminal cell periphery. The utilization of sugars by carcinomas was much more variable. Differences were observed between adjacent cells and cell groups of the same tumour. The uptake of individual sugars within a carcinoma was also varied being either similar to, or greater or lesser than normal breast. Variation between carcinomas was also present. No correlation between type and differentiation was noted in this respect, but there was between localization of sugars and differentiation. Better differentiated areas in tumours showed patterns similar to non‐malignant breast whilst localization in poorly differentiated cell groups was cytoplasmic. The uptake of leucine was more constant and proved to be a useful indicator of viability. While this approach cannot give information with regard to differences in glycoprotein structure between non‐malignant and malignant breast, it has been of value in determining the heterogeneity of tumour cells with regard to the enzymes involved in glycosylation. As such it would be of use in assessing the uniformity of response to agents modifying glycosyl

ISSN:0022-3417    

DOI:10.1002/path.1711490303

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

摘要:

AbstractEleven orthotopically transplanted human hearts have been examined at retransplantation or necropsy.They were selected to cover the period up to 3 years after transplantation. The recipient SA node was examined in six, the donor SA node in eight and the AV conduction tissue in all eleven. Each of these areas has been examined by serial sectioning. All the conduction tissue is discretely involved in rejection and this involvement is no more severe than in the adjacent myocardium. In the AV tissue there is a tendency for the peripheral parts to be more frequently involved than the proximal. There is little evidence of permanent structural damage to the AV tissues by recurrent episodes of mild to moderate rejection. In the atrial nodes there is about a 50 per cent chance of surgical or procurement damage to both the recipient and the the donor tissue.

ISSN:0022-3417    

DOI:10.1002/path.1711490304

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

摘要:

AbstractLectin binding patterns to the developing pulmonary vasculature were studied in 10 Large White pigs aged 1 min to 1 week and in three adult animals. Paraffin‐embedded tissue sections were exposed to eight lectin peroxidase conjugates:Dolichos biflorus, Triticum vulgaris, Concanavalin A, Ricinus communistype 2,Arachis hypogaea, Ricinus communistype 1,Tetragonolobus purpureasandUlex europeus. Lectin binding patterns to the pulmonary arterial and venous endothelium, to smooth muscle cells (SMCs) and to the arterial connective tissue were age‐related. Changes occurred during the first week of life and between 1 week and adult life. Neither the endothelial binding patterns in the adult nor the SMC patterns in the immature and adult lung conformed to known morphological differences between the different segments of the arterial and venous pathways. Heterogeneity for endothelial binding was seen in the immature lung. These studies indicate biochemical differences in surface structure between the endothelium, SMCs and connective tissue of the immature and mature lung. Ultrastructural localization of the lectins in the vasculature of the developing animal lung ought to help interpret similar data obtained on the vessels of the immature human pulmonary hypertensive lung using lectins which show similar binding patterns in both spec

ISSN:0022-3417    

DOI:10.1002/path.1711490305

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711490306

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711490301

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY