ISSN:0022-3417    

DOI:10.1002/path.1711620102

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711620103

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711620104

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

摘要:

AbstractParaffin sections of 66 surgically resected lung tumours were immunostained with antisera to human endothelin‐1 and to the C‐terminal peptide of big endothelin. With both antisera, strong immunoreactivity was demonstrated in 11 of 15 squamous cell carcinomas and 11 of 16 adenocarcinomas. Focal immunoreactivity was seen in small cell carcinoma (2/2), large cell carcinoma (2/5), and carcinoid tumours (2s/11). Four lymphomas and three sarcomas did not show endothelin immunoreactivity. Cryostat sections of 22 of the 66 tumours were hybridized with radiolabelled complementary RNA probes prepared from the 3′ non‐coding region of endothelin‐1 cDNA, and the chromosomal genes encoding endothelin‐2 and ‐3.In situhybridization demonstrated the presence of endothelin mRNAs in 4 of 7 squamous cell carcinomas and in 5 of 8 adenocarcinomas, in a pattern similar to that shown by immunocytochemistry. No hybridization signals were obtained from the other types of tumours. In lung tissue adjacent to the tumours, endothelin‐like immunoreactivity and mRNA were detected in pulmonary endocrine cells and, in some cases, other epithelial cells, and in alveolar capillary endothelial cells. This study demonstrates the expression of endothelin in a number of pulmonary tumours and suggests a possible role for this peptide in the growth and/or differentiation o

ISSN:0022-3417    

DOI:10.1002/path.1711620105

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

摘要:

AbstractAntibodies against the carbohydrate antigens Lewisa(Lea) and Lewisb(Leb) allow further investigation into the changes in fucosylation of the glycocalyx occurring during the normal and neoplastic development of cervical squamous epithelium. Leaand Lebare expressed on a broad zone of suprabasal cells in normal cervical squamous mucosa often independent of individual Lewis gene and secretor status. A proportion of Leais expressed in sialylated form. In cervical intraepithelial neoplasia, the progressive dedifferentiation of the squamous mucosa is mirrored by loss of both Leaand Lebexpression. In invasive squamous carcinomas, Leaand Lebexpression is seen only on cells in areas of differentiation.The expression of Leaand Lebin normal and neoplastic cervical squamous epithelium resembles that of the Lexantigen described by us previously. The structural similarity betweenthese antigens is highlighted and evidence for a common functional role in maintaining the integrity of squamous mucosae is discussed.

ISSN:0022-3417    

DOI:10.1002/path.1711620106

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

摘要:

AbstractThe immunoreactivity of a range of vascular and non‐vascular smooth muscle tumours, rhabdomyosarcomas, and non‐myoid lesions has been examined with the use of a monoclonal antibody to smooth muscle‐specific actin and the muscle intermediate filament, desmin. In all cases of smooth muscle‐derived tumours, the alpha‐actin antibody yielded superior results. Staining of the myofibroblasts of fibromatoses was also seen. In contrast to desmin, immunoreactivity was not exhibited by rhabdomyosarcomas. We propose that this monoclonal antibody to alpha‐smooth muscle actin is a useful addition to the panel of reagents used for the characterization of soft tissue proliferations and tumours. The technical aspects of the application of this monoclonal antibody to immunohistochemistry ar

ISSN:0022-3417    

DOI:10.1002/path.1711620107

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

摘要:

AbstractThirty‐five biopsies from 19 patients with superficial transitional cell carcinoma of the bladder, treated with intravesical bacillus Calmette‐Guérin (BCG), were assessed histologically and immunohistochemically. Pretreatment biopsies were available for comparison in all cases and five cases of non‐specific cystitis were also examined. The inflammatory infiltrate was assessed with a streptavidin‐biotin‐peroxidase method using UCHL1, MT1, LN3, L26, HAM56, MAC387, Leu7 and anti‐S100 in paraffin sections, and in 18 specimens where frozen tissues were available, Leu 1,2,3,4,14, OKT10, HLA‐DR and anti‐Tac antibodies were applied. Post‐treatment bladder biopsies showed severe oedema and a variable degree of inflammation. A granulomatous inflammation was seen in 11 cases, with granulomas present in six prostatic biopsies and acid‐fast bacilli in two cases. The lymphoid infiltrate in all biopsies were largely T lymphocytes with a predominance of T helper cells present, often as a band‐like infiltrate pressing against the residual epithelium, or the denuded bladder surface, and distributed in the vicinity of the granulomas. Activated lymphocytes were prominent in seven cases, although a moderate infiltrate of such cells was seen in all instances. Tac antigen was only occasionally expressed, and in a few NK cells were present among the infiltrates. In eight cases, HLA‐DR was expressed in epithelial cells following BCG treatment, whereas all pre‐treatme

ISSN:0022-3417    

DOI:10.1002/path.1711620108

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

摘要:

AbstractUsing a one‐step silver nitrate staining technique, routinely processed tumour tissues of 49 carcinomas of the colon were investigated to demonstrate silver‐stained nucleoli (Ag‐nus) and argyrophilic proteins associated with the so‐called nucleolar organizer regions (Ag‐NORs). Patients with attempted curative resections and tumour stages Dukes' A, B, C1 and C2, with an uneventful follow‐up period of at least 48 months (N= 17), showed a statistically significant (P= 0·0001) lower mean number of scattered Ag‐NORs (3·04; SD: 1·08) compared to patients who developed metastases during their follow‐up period (N= 15; 5·40; SD: 1·28), as well as to patients who underwent palliative surgical treatment (N= 17; 4·48; SD: 1·67). Mean numbers of scattered Ag‐NORs per nucleus and staging of the tumour were strongly related (P= 0·0001) to cancer‐specific survival. The results indicate that the evaluation of silver‐stained particles according to their different distribution patterns is of great value with regard to the clinical outcome of colonic carcinoma and may even allow a more accurate prognostic assessment of these patients than the WHO grading system, UICC staging system, the so‐called Jass‐scoring

ISSN:0022-3417    

DOI:10.1002/path.1711620109

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

摘要:

AbstractIn a retrospective study of 70 patients with malignant pleural mesothelioma, 168 formalin‐fixed, paraffin‐embedded tumour specimens were examined for DNA content by flow cytometry. In 20 patients where two or more blocks of the same tumour were available, there was considerable agreement between ploidy status and S‐phase percentage in the different specimens.There were no significant differences for survival for patients who had been exposed to asbestos and those in whom no exposure could be elicited, nor for aneuploid and diploid tumours. The S‐phase content was examined for different areas of the same tumour and the percentages were largely in agreement. However, those patients who had tumours with an S‐phase percentage greater than the median (6 per cent) had a significantly shorter survival than those with tumours of lower S‐phase percentage. Differences in DNA content and other cell cycle parameters were not associated with the histologic

ISSN:0022-3417    

DOI:10.1002/path.1711620110

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY

摘要:

AbstractA computer‐aided morphometrical study was performed on histological specimens of reactive hyperplastic (n=10) and malignant (n=17) mesothelium. For each cell, seven nuclear features were measured and 13 parameters computed. Using stepwise variable selection, discriminant analysis chose the nuclear contour index, the standard deviation of the nuclear area, and the mean of the nuclear perimeter as discriminating features between hyperplastic and malignant mesothelium. The coefficients of these variables were included in a discriminant function which gave perfect discriminantion between the two groups of lesions. When the function was assessed on a test set of hyperplastic (n= 10) and egories was achieved. This classification rule may help to increase the level of confidence with which a histological diagnosis of mesothelioma can be establishe

ISSN:0022-3417    

DOI:10.1002/path.1711620111

出版商:John Wiley&Sons, Ltd.    

年代:1990

数据来源: WILEY