ISSN:0022-3417    

DOI:10.1002/path.1711570202

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711570203

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractLung alveolar macrophages (AM) and blood monocytes (BM) are part of the mononuclear phagocyte system (MPS) and have been shown to be composed of several functionally and biochemically distinct subsets. AM and BM were each fractionated into four subfractions by centrifugation over Percoll, 95 per cent of the monocytes having a higher buoyant density than the high‐density AM fraction. Antigen distribution within the cell subfractions was studied by an immunofluorescence technique using a panel of ten monoclonal antibodies (MoAbs) reacting with separate antigenic determinants previously found on mononuclear phagocytes. Several antigens were present on both types of cells (Ki‐M1, Ki‐M6, Leu‐M3, Leu‐M5, 1D5, Dako‐Ma, and HLA‐DR), while other antigens were expressed exclusively on BM (MAS 072 and MAS 081) or on AM (Ki‐M8). Ki‐M1, Leu‐M3, Leu‐M5, and HLA‐DR were expressed to a greater degree on the surface of AM (95–96 per cent of cells) than on BM (5668 per cent), while an inverse relationship was noted for 1D5 antigen (present on 41 per cent of BM and 11 per cent of AM). Ki‐M6 and Dako‐Ma MoAbs recognized an intracellularly restricted antigen present in both AM and BM. Ki‐M1, Ki‐M8, MAS072, MAS081, Leu‐M3, Leu‐M5, 1D5, and HLA‐DR were expressed both in the cytoplasm and at the cell surface. Some antigens (1D5, Leu‐M3, MAS 072, MAS 081, and Dako‐Ma) showed an uneven distribution among subsets of both AM and BM. The variability found in the expression of both membrane and intracellular antigens underlines the remarkable heterogeneity among mononuclear phagocytes and emphasizes the potential value of MoAbs to study the sequential stages of maturation/differentia

ISSN:0022-3417    

DOI:10.1002/path.1711570204

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractThe duration of the S phase of the cell cycle (ts) in the glands and stroma of normal, hyperplastic, and malignant endometrium was measuredin vitrousing a double labelling technique. Endometrial organ cultures were incubated sequentially in tritiated thymidine (3HTdR) and bromodeoxyuridine (BrdU) each for 1 h. Labelled cells were visualized in tissue sections using3HTdR autoradiography and immunohistochemistry with an anti‐(BrdU) antibody. Three populations of cells, single labelled with either3HTdR or BrdU, and double labelled cells, were easily identified and tswas calculated from both the rate of influx into, and efflux out of S. There were no significant differences in tsin the different tissue types or between influx and efflux in the glands. However, in the stroma tscalculated from the rate of efflux was significantly shorter than that calculated from the rate of influx, suggesting that during the cell culture period the rate of cell efflux from S was greater than influx. This is the opposite to what would be expected in an exponentially growing cell population and suggests that there may be an effect from the culturing condition

ISSN:0022-3417    

DOI:10.1002/path.1711570205

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractIn an autopsy series of 8571 cases from 1975 to 1984, cancer deaths increased significantly, particularly in those cases over 60 years of age. A similar trend was seen in the mortality statistics from the district. Study of the degree of correlation between the frequency for the different types of cancer and the total deaths from that cancer at autopsy in the population supplies guidelines for use in assessing the relevance of autopsy findings to epidemiological research. It is stressed that without autopsy control monitoring of cancer in a population is incomplete and unreliable. An autopsy frequency of 40 per cent or more for the cancer in question in the population as a whole is required combined with series of 5–600 cases if satisfactory studies are to be initiated. Such conditions are difficult to fulfil for the less common types, but offer a valid approach to the monitoring of those types of cancer that are numerous enough to merit such attentio

ISSN:0022-3417    

DOI:10.1002/path.1711570206

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractPulmonary endocrine cells have been studied according to their immunoreactivity for neuron‐specific enolase, gastrin‐releasing peptide (GRP), calcitonin, leucine‐enkephalin, and serotonin in the lungs of subjects with chronic bronchitis and emphysema. The overall population was significantly greater in comparison with matched controls. The change was most marked in lobes affected by pneumonic consolidation, and did not affect equally the sub‐populations of cells as identified by their content of peptide; the difference was much greater for calcitonin‐containing cells than for those immunoreactive for GRP. This change may be the basis of the hypercalcitoninaemia and hypercalcitoninuria which have been reported in patients with inflammatory pulmonar

ISSN:0022-3417    

DOI:10.1002/path.1711570207

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractJC virus genomes have been localized in formalin‐fixed, paraffin‐embedded brain tissues of two cases of known progressive multifocal leukoencephalopathy byin situhybridization utilizing a biotinylated JC virus DNA probe. A three‐stage immunoperoxidase system with gold–silver amplification of the diaminobenzidine substrate was used to visualize biotinylated nucleic acid hybrids. Dot–blot quantification of this visualization system indicates that subpicogramme amounts of biotinylated DNA can be detected. Optimal detection of the virus genomes in the brain tissues required a microwave irradiation step prior to hybridization. JC virus genomes were observed in the nuclei of enlarged oligodendrocytes and of some bizarre astrocytes. No other cell types were found to harbour th

ISSN:0022-3417    

DOI:10.1002/path.1711570208

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractFrom 133 to 615 glomeruli were examined in sections of kidneys from each of 60 animals, representing six rodent models of proteinuria. Particular attention was paid to the position of segmental lesions. Lewis rats given sheep anti‐rat glomerular basement membrane antibodies had lesions almost exclusively at the glomerulo‐tubular junction. Wistar rats on a diet of 24 per cent casein or with subtotal nephrectomy and a diet of 24 per cent soya had lesions mainly at the hilum. Wistar rats given bovine serum albumin had global lesions but virtually no segmental lesions. Wistar rats given puromycin aminonucleoside had lesions at the glomerulo‐tubular junction and global mesangial abnormalities shortly after the treatment but later developed segmental lesions at all parts of the glomerulus. Untreated BUF/Mna rats had lesions at the glomerulo‐tubular junction early in life but later had lesions at all parts of the glomerulus. Untreated NZB/NZW hybrid mice had various types of glomerulonephritis and also had lesions at the glomerulo‐tubular junction. These findings showed that (1) segmental lesions at the glomerulo‐tubular junction, or glomerular tip, occur in experimental animals, a fact not previously reported, and these tip changes are a common feature in several different models of proteinuria; (2) hilar segmental lesions are seen in conditions with hyperfiltration of protein; and (3) segmental lesions at various parts of the glomerulus are seen in some models of proteinuria and probably indicate late effects of random toxic damage to the glomerulus. Thus, there are at least three different types of segmental glomerular lesions in experimental animals—tip, hilar, and random—with different morphology and pathogenesis. It is likely that these findings can be extended to human renal diseases with segmental glomerular lesions. This will help to clarify the controversial and unsatisfactory term focal segmental glo

ISSN:0022-3417    

DOI:10.1002/path.1711570209

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711570210

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711570211

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY