ISSN:0022-3417    

DOI:10.1002/path.1711560202

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711560203

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711560204

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractLuminal narrowing was assessed in 238 transverse segments obtained from coronary arteries removed at postmortem. In each segment, narrowing was assessed by gross visual estimation before and after fixation, and on histological sections by stereological point counting and computer‐assisted planimetry.Computer‐assisted planimetry was found to be accurate and reliable but the equipment needed is expensive, and requires specialized software and an experienced user.Morphometric measurement by stereologic point counting was accurate, rapid, simple, and inexpensive.In comparison with computer‐assisted planimetry visual estimation was found to be neither accurate nor reliable.Our results indicate point counting as the method of choice for assessment of coronary artery luminal narrowing by atheroscle

ISSN:0022-3417    

DOI:10.1002/path.1711560205

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractMicroscopically assessed changes observed in large sections of osteosarcomas from patients who had undergone pre‐operative chemotherapy (PCT) (n=22), or no chemotherapy (n=22), were examined either quantitatively, semi‐quantitatively, or qualitatively. Eight tumour characteristics were associated with the treatment mode by way of a stepwise logistic analysis. Minimal amount of viable tumour tissue (<10 per cent) and strong fibroblastic proliferation were each proved to be associated with PCT. These two variables in combination indicated a good response to PCT. The discrimination between PCT and non‐PCT patients based on statistical analysis was superior to discrimination based on ‘overall impr

ISSN:0022-3417    

DOI:10.1002/path.1711560206

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractThe characteristics of the germinal matrix vasculature were studied in the developing fetal brain using immunocytochemical methods. A preliminary comparative immunocytochemical study was made on six fetal brains to compare endothelial staining byUlex europaeusI lectin with that of antibody to Factor VIII related antigen.Ulexwas found to stain germinal layer vessels better than Factor VIII related antigen. Subsequently, the germinal layers of a further 15 fetal and preterm infant brains ranging from 13 to 35 weeks' gestation were stained withUlex europaeusI to demonstrate the vasculature. With increasing gestation, there was a gradual increase in vessel density, particularly of capillaries. This was not a uniform process. A plexus of capillaries was prominent immediately beneath the ependyma while the more central parts of the germinal matrix contained fewer, but often larger diameter, vessels. The variation in vessel density which was a feature of the later gestation brains may have implications for local blood flow and may be a factor in haemorrhage at this site.

ISSN:0022-3417    

DOI:10.1002/path.1711560207

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractWe have used a chronic serum sickness model of glomerulonephritis to investigate whether gross interference with the immune system can influence the rate of removal of antigen and established electron‐dense deposits from the glomerulus. Radio‐labelled cationized bovine serum albumin (BSA) was used as antigen. During the 2 weeks after the cessation of injections, the rate of removal of antigen from isolated glomeruli and from renal cortex, liver, spleen, and lung was measured. The rate of removal of mesangial and subepithelial deposits was assessed by point‐counting. Urinary excretion of free and protein‐bound isotope was also measured.Having quantified the rate of removal of antigen and deposits from the glomerulus, we attempted to influence the rate of removal by interfering with the immune response in the course of recovery. Contrary to our expectations, stimulation of the immune system with antigen in Freund's complete adjuvant, 4 days after the last injection of antigen, inhibited the removal of antigen and mesangial electron‐dense deposits. Prednisolone had no detectable effect, but large doses of a non‐nephritogenic form of the antigen (native BSA) enhanced removal. Removal of antigen and mesangial deposits was inversely correlated with the levels of circulating anti‐BSA antibody suggesting that specific antibody, circulating through the mesangium, inhibits the removal of antigen which is already trapped at that site. None of the forms of intervention applied during recovery produced a detectable change in the rate of removal of subepithe

ISSN:0022-3417    

DOI:10.1002/path.1711560208

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractTherasoncogenes encode for GTP binding and GTPase active proteins of relative molecular mass 21 000 (p21ras) which are involved in the transduction of stimuli for cell proliferation. There have been conflicting reports about the detection and significance of expression of p21rasprotein in human breast disease as determined by immunohistochemistry.The antibody Y13‐259, which detects a single protein ofMr21 000, has been applied immunohistochemically to frozen sections of normal, benign proliferative breast, fibroadenomas, and carcinomas. Uniform staining of normal breast epithelium and myoepithelium was found, with occasional stronger staining in areas of epithelial hyperplasia in benign breast disease. Contrary to previous reports, decreased expression, usually heterogeneous, was found in half of the carcinomas examined. Thirty per cent of the carcinomas exhibited heterogeneous staining stronger than that of normal breast, interpreted as increased expression of p21rasprotein. This did not relate to tumour grade or node status but showed a significant correlation with proliferation rate as determined by the monoclonal antibody Ki‐67.This study confirms previous reports that p21rasprotein expression is a feature of normal cells, and has identified increased expression in 30 per cent of tumours associated with higher proliferation rates, which is a lower incidence than previously claimed when a different antibody was emplo

ISSN:0022-3417    

DOI:10.1002/path.1711560209

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractLiver biopsies from nine patients with maturity‐onset diabetes and fatty liver hepatitis were semiquantitively assessed, and the findings compared with those in alcoholic hepatitis. Overall appearances were similar, but the lesion in some diabetics was periportal rather than perivenular in location, and nuclear vacuolation of hepatocyte nuclei was alway present. The inflammatory infiltrate often included neutrophil leucocytes, as in the alcoholic. In three patients with multiple biopsies, progression appeared to be slow, but one patient developed cirrhosi

ISSN:0022-3417    

DOI:10.1002/path.1711560210

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractUntil recently, [3H]‐thymidine incorporation, DNA analysis by flow cytometry, and cell doubling times have been the main methods of studying tumour cell kinetics. All these techniques are laborious, expensive, and difficult to perform in a routine diagnostic laboratory. This study examined fresh frozen sections from 31 prostatic biopsy specimens with the hybridoma antibody Ki‐67, a marker of proliferating cells, using a modified avidin—biotin—peroxidase complex technique. The percentage of glandular cells decorated by this antibody, representing the growth fraction, was determined for both benign and malignant samples. Benign prostatic glands showed an average Ki‐67 score of 4 per cent, significantly less than the 16·3 per cent mean growth fraction found in prostatic carcinomas. There was a significant correlation between the tumour growth fraction as assessed by Ki‐67 staining, and the histological grade. A positive correlation was also found between the Ki‐67 score and the intensity of staining, and a definite trend was noted between the Ki‐67 score and the tumour clinical stage. Ki‐67 promises to be a useful marker in determining the prognosis o

ISSN:0022-3417    

DOI:10.1002/path.1711560211

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY