ISSN:0022-3417    

DOI:10.1002/path.1711540202

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711540203

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractThe existence of a distinctive form of chronic gastritis characterized by marked infiltration of the surface and pitlining epithelium by mature T lymphocytes has been confirmed. Seventeen cases were identified amongst 382 patients with active chronic gastritis (4·5 per cent). The cases with lymphocytic gastritis had significantly higher counts of intra‐epithelial lymphocytes than sex and age‐matched controls drawn from a series of patients with the usual form of active Type B chronic gastritis. Furthermore, the lymphocytic gastritis group exhibited unusual endoscopic findings, namely erosions and ‘raised lesions’, in the body of the stomach. Such appearances have been previously described as ‘varioliform’ gastritis. Only seven of the patients (41 per cent) hadC.pylori‐positive biopsies, compared with over 90 per cent in the generality of active chronic gastritis, but all but two of the eleven cases tested had serological evidence ofC. pyloriinfection. While the pathogenesis of this condition is unclear, the histological resemblance to coeliac disease suggests that lymphocytic gastritis might also represent an abnormal response to a local antigen to which the patient has become sensitized. It is possible that this antig

ISSN:0022-3417    

DOI:10.1002/path.1711540204

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractUsingin situimmunohistological analysis, expression of Leu‐8 and its correlation with other B‐cell markers were investigated in 21 selected lymphomas of different categories, each one expressing its own typical immunophenotype. These categories included eight follicular centroblastic/centrocytic (CB/CC) lymphomas, eight intermediately differentiated lymphocytic lymphomas (ILL)/mantle zone lymphomas (MZL), and five lymphocytic lymphomas (LL) associated with chronic lymphocytic leukaemia (CLL). Four reactive lymph nodes and three tonsils were also studied using double immunolabelling procedures. Cell suspensions were also performed in three CB/CC and four ILL/MZL cases. Leu‐8 was consistently expressed in ILL/MZL and LL but it was absent in most (7/8) CB/CC lymphomas. In reactive tissues, the Leu‐8‐positive B cells were strictly confined to the mantle zones. A close association emerged between Leu‐1 (CD5) and Leu‐8, both being present in ILL/MZL and LL but absent in CB/CC. A consistent lack of association was found between Leu‐8 or CD5 antigens and common acute lymphoblastic leukaemia antigen (CD10) and BA‐2 (CD9) antigen, whereas Leu‐8 and CD5 were strictly associated with surface IgD. Reactivity with Leu‐8 provides a means of distinguishing between CB/CC and ILL/MZL. Furthermore, shared immunoreactivity for Leu‐8 in ILL/MZL and LL may represent a potential clue to the still uncertain cellular

ISSN:0022-3417    

DOI:10.1002/path.1711540205

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractPairs of bone marrow trephine samples from 67 patients with known or suspected non‐Hodgkin's lymphoma (NHL) were collected. One sample was processed for morphological examination, the other for frozen section immunohistology, using a panel of monoclonal antibodies (MAB) reacting with lymphocyte and macrophage determinants, detected by the immuno‐alkaline‐phosphatase (APAAP) method. Forty‐one cases showed definite (36) or suspected (5) involvement of the marrow by NHL. Most were examples of lymphocytic or centroblastic/centrocytic NHL. The pattern of immunostaining confirmed the presence of NHL in these cases: the phenotype of the neoplastic cells was broadly consistent with nodal histology, where available. In ten cases, the marrow showed no evidence of involvement by NHL, and in 15, the eventual diagnosis was an abnormality of the myeloid series. These two groups of marrows not involved by NHL both showed a ‘reactive’ pattern of immunostaining, comprising polyclonal B‐cells, T‐helper and suppressor/cytotoxic cells and macrophages. We conclude that immunohistological examination of the bone marrow is useful in cases where the specimen shows morphological evidence of NHL, including those that are only ‘suspect’, but does not detect lymphoma where there is no morphological evid

ISSN:0022-3417    

DOI:10.1002/path.1711540206

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractThe argyrophilic staining (AgNOR) technique, novel in histopathology, was applied to a series of 20 non‐Hodgkin's lymphomas (NHL) of established Kiel subtype. The method demonstrates nucleolar organizer regions (NORs) by virtue of sulphydryl groups on their associated proteins and the enumeration of AgNOR foci has been previously shown to discriminate between NHL of low‐ and high‐grade histological types. This finding was confirmed and the results were compared with those obtained by means of DNA flow cytometry performed on paraffin wax‐embedded tissue from the same lymphomas. There was a very good linear correlation between the mean numbers of AgNOR sites per nucleus and the percentage of S‐phase cells for each case, both values being high in high‐grade NHL and low in low‐grade lesions. Conversely there was no significant correlation between the DNA index, representing DNA aneuploidy, and AgNOR counts. It is suggested that the numbers of AgNORs in a lymphoma may be related to the dividing fraction of cells rather than, as might be expected, to ploidy alone. It is also proposed that the AgNOR technique, which is rapid, simple, and inexpensive, may provide, at least, an adjunct to DNA flow cytometry in the assessment of neoplasm in h

ISSN:0022-3417    

DOI:10.1002/path.1711540207

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractSeven consecutive cases of primary spindle celled tumours of the breast have been studied immunohistologically using antisera to the intermediate filament proteins (IFP) vimentin, cytokeratin, and desmin, and with an antibody to epithelial membrane antigen. Representative paraffin sections were examined using a peroxidase–antiperoxidase method. In three cases, very occasional foci of epithelial differentiation were apparent by conventional microscopy, and in one case, adjacent ductal carcinomain situwas present. The remaining three cases were composed of spindle cell elements entirely, with no evidence of epithelial differentiation morphologically. Immunoreactivity of spindle cell elements for vimentin was found in all seven cases, and for cytokeratin in six cases. One case showed immunoreactivity for vimentin, cytokeratin, and desmin, and one case only for vimentin. Epithelial membrane antigen was not identified in the spindle cell elements of any tumour, but was present in the invasive epithelial component of three cases and thein situcomponent of one case. We conclude that many spindle cell tumours of breast show immunohistological evidence of epithelial differentiation and can be regarded as spindle cell carcinomas. However, in some cases IFP expression may be complex and histogenesis cannot be determined. This technique can aid histological diagnosis in some case

ISSN:0022-3417    

DOI:10.1002/path.1711540208

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractFor histological control of excisional margins, routinely fixed tumour specimens or, under certain conditions, specimens fixed immediately in warm formalin are processed in the histology laboratory. Strips are then cut from the undersurface, edge, and cross‐section of the remainder of the specimen and processed further by routine paraffin techniques until H&E‐stained sections of the entire periphery and midsection are available. We suggest that the method is simple, easy to apply, and suitable for the assessment of excisional margins of a variety of skin tumours. Extremely low recurrence rates over a relatively long follow‐up period substantiate the effectiveness of the m

ISSN:0022-3417    

DOI:10.1002/path.1711540209

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractRichardson's ground squirrels (Spermophilus richardsonii) of both sexes were fed a 2 per cent cholesterol‐enriched diet for intervals of 12, 18, and 24 h; 3, 5, and 7 days; and 2, 3, 10, and 20 weeks. It was shown that free (unesterified) cholesterol, phospholipid, and cholesterol ester accumulated in specific regions of the gallbladder mucosa during cholelithiasis. Electron microscopy revealed the presence of lipids inter‐ and intracellularly as early as 12 h after ingestion. By 7 days, lipids were seen in dilated endoplasmic reticulum, as well as in supranuclear and basal regions of epithelial cells. Histochemical localization revealed free cholesterol in dilated endoplasmic reticulum and residual bodies at the ultrastructural level. Neutral lipid was observed by light microscopy in the supranuclear and basal regions of the cells. In 10‐ and 20‐week treated animals, lipid droplets were also seen in the lamina propria and macrophages. The lesion induced by cholesterol ingestion persisted throughout the experimental period, and while different from that in human tissue, it was similar to those observed in experimental canine choles

ISSN:0022-3417    

DOI:10.1002/path.1711540210

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractAdult Sprague–Dawley rats received a daily intraperitoneal injection of dexamethasone (100 or 200 μg/kg) for 2 or 6 weeks. Quantitative assessment of the anterior pituitary corticotroph population was performed using the stereological measurement of volume density (Vv). An indirect assessment of corticotroph function was also made, based on adrenal weight and histology. Adrenal changes were consistent with dose‐related inhibition of corticotroph function in all dexamethasone‐treated animals. Immunopositive corticotrophs were easily identified and in no treatment group was corticotrophVvsignificantly less than in the appropriate vehicle‐injected control group. In male animals, there was a significant increase (P<0·05) inVvwith 100 μg/kg at both 2 and 6 weeks. These results suggest that it is possible to produce inhibition of corticotroph function by the administration of such doses of dexamethasone without causing a significant reduction in the anterior pituitary corticotroph population. This may be related to the mode of negative feedback of suc

ISSN:0022-3417    

DOI:10.1002/path.1711540211

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY