摘要:

AbstractSeven per cent (10/145) of hybridomas raised against partially purified activated glucocorticoid receptor from rat liver produced monoclonal antibody to receptor. Six IgM secreting clones selected for further investigation bound equally well to activated and non‐activated receptor from fresh rat liver, but significantly less well (11–25 per cent) to receptor from frozen rat liver. No interaction was found with oestrogen receptor from rat uterus but extensive cross reaction occurred with progesterone receptor. Although none of the antibodies bound to glucocorticoid receptor from human or porcine liver or lymphoid cells, several cross‐reacted with mouse liver glucocorticoid receptor. Immunoelectroblotting of proteins from fresh and frozen rat liver cytosol showed the antibodies bound to 90 000 and 40 000 MW forms of receptor respectively. Immunostaining of both frozen and paraffin embedded sections of rat tissue showed that receptor is preserved during fixation and processing of tissues. Using both indirect immunoperoxidase and immunogold silver staining methods, the pattern of receptor staining observed correlates with the known glucocorticoid responsiveness of the tissues st

ISSN:0022-3417    

DOI:10.1002/path.1711500402

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

摘要:

AbstractThe origin and mechanism of formation of the osteoclast remains controversial. Although it is known to be derived from a circulating mononuclear precursor, the identity of this cell is unknown. Using a panel of monoclonal antibodies raised against macrophage and other marrow‐derived cells, we determined the immunocytochemical staining of human osteoclasts in both fetal bone metaphyseal imprints and frozen sections. Osteoclasts and marrow mononuclear cells were stained by three broad spectrum antimacrophage antibodies, EBM‐11, Y182a and BM2. T310, an antibody which stains macrophages and T helper cells, and C17, an antimegakaryocyte antibody, also stained osteoclasts. EBM‐11, Y182a and BM2 also stained megakaryocytes in bone imprints as well as normal bone marrow smears. The presence of macrophage‐associated antigens in osteoclasts, megakaryocytes and bone marrow mononuclear cells indicates that they are phenotypically similar to macr

ISSN:0022-3417    

DOI:10.1002/path.1711500403

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

摘要:

AbstractIn this paper we present a further case of a new clinicopathological entity combining a rare type of non‐Hodgkin lymphoma with a myelomonocytic leukaemia. The characteristic feature of the lymphoma is massive infiltration of the T‐zones of lymph nodes by plasmacytoid cells originally described by Lennert1in non‐specific lymphadenitis. Two lymphoma cases of this type have recently been published, by Müller‐Hermelinket al.2who named the cells ‘plasmacytoid T‐cells’ (PTC), and by Prasthoferet al.3These three cases have similar clinical and pathological features and appear to form a distinct clinicopathological entity. In contrast to the two previously published cases the present lymphoma also contained irregular lymphoid cells accompanying the PTC in the lymph node lesion and focally infiltrating the bone marrow. An accumulation of polytypic IgG positive plasma cells was observed in the remaining lymph node follicles. Immunohistological analysis with a range of monoclonal antibodies showed the PTC of our case to be CD5(T1) +, CD4(T4) +, CD3(T3)‐, CD8(T8)−, CD2(T11)−, and CD25(TAC)−, but HLA‐DR + and transferrin receptor positive. The nature of this peculiar lymphoid lesion and its relationship to myelomonocytic

ISSN:0022-3417    

DOI:10.1002/path.1711500404

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

摘要:

AbstractThe histopathological features of the acute infection byCandida albicansin susceptible CBA/H mice, as well as its dissemination in various organs has been examined. The brain, in which the localization of blastospores is minimal, is the organ most severely affected. A significant deposition of blastospores occurs in lungs, liver, spleen and kidney, but of these organs, only in the kidneys is infection established. A transient myocarditis follows the minor blastospore deposition that occurs in the heart. Infection in the affected organs is characterized by abscess formation while in the brain the process is complicated by the development of hydrocephalus and a pachymeningitis that erodes the petrous temporal and involves the vestibular apparatus. There is also infection of the retinae with an accompanying endophthalmitis.

ISSN:0022-3417    

DOI:10.1002/path.1711500405

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

摘要:

AbstractFixed anionic charges in the mammalian glomerulus, on both the basement membrane and the eptihelial cell foot processes, are believed to form an important part of the glomerular filtration barrier. There is good evidence that their loss causes proteinuria. The charges can be visualized ultrastructurally using cationic dyes, but the requirement of these techniques for perfusion or immersion of fresh tissue has largely confined such studies to experimental models.We have extended the widely used polyethyleneimine technique, to study the charge of glomerular basement membranes in human tissue reprocessed out of paraffin blocks up to 10 years old. We studied selected cases of glomerular disease, where the diagnosis was not in any doubt. In the majority of diseases studied, a continuous charge layer persisted despite severe abnormalities of the basement membrane. Two exceptions were found. In amyloidosis, accumulation of fibrils was associated with a considerable decrease or loss of stainable basement membrane charge. In S.L.E., numerous small defects in the charge layer were noted.The persistence of charge is contrary to reported findings in several animal models of glomerular disease, including puromycin nephrosis, Heymann nephritis and streptozotocin diabetes. Although this method is not subject to precise quantitative analysis, we conclude that in the majority of cases, proteinuria in man is not caused by an extensive loss of glomerular basement membrane anionic charge.

ISSN:0022-3417    

DOI:10.1002/path.1711500406

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

摘要:

AbstractAcute endothelial injury induced by bleomycin has been shown to enhance the localization and metastasis of circulating tumour cells. In the present study we wished to determine whether increased metastases to the lung is related to the degree of endothelial damage as indicated by morphology and protein leakage to alveoli and whether the progression to repair with pulmonary fibrosis also effects metastatic tumour growth. C57b1/6 mice were injected with a single intravenous dose of bleomycin (120 mg/kg). After 5 days, severe enothelial injury was demonstrated by morphology and by increased levels of protein in lung lavage fluid. When [131I]‐iododeoxyuridine labeled syngeneic fibrosarcoma cells were injected intravenously at this time, a 9‐fold increase in their localization was detected 24 h later in bleomycin‐treated lungs compared with saline controls. By electron microscopy tumour cells were observed at sites of denuded vascular basement membrane. There was also a significant increase in the number of gross metastases which developed subsequently and in the percentage of lung occupied by tumour in the bleomycin group. Animals examined 10 days after bleomycin showed less endothelial damage and a smaller increase in tumour cell localization and metastases. At 21 days, when endothelial structure and alveolar protein levels had returned to normal, and at 6 weeks, when there was focal fibrosis, no increase in tumour cell localization or metastases was found. It is concluded that damage to the pulmonary endothelium is a key factor in enhancing the trapping of circulating tumour cells and increasing metastatic tumour growth after bleo

ISSN:0022-3417    

DOI:10.1002/path.1711500407

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

摘要:

AbstractWe have investigated the distribution of an apocrine membrane antigen (AEA) in pulmonary tissue using a rabbit antiserum raised against fat globule glycoproteins isolated from human milk. In indirect immunostaining (PAP, IF) of sections from normal lung tissue, the membranes facing the alveolar lumen of cells corresponding to the type II pneumocytes in the alveolar walls were decorated. The selective distribution of AEA to the membranes of type II pneumocytes was confirmed in double immunostaining by identification of these cells with rat antibodies against surfactant apoprotein.In fetal lung tissue, the AEA antigen was detected by the 9th week of gestation. In lung samples from newborns which had died of respiratory distress syndrome (RDS) the intra‐alveolar hyaline membranes stained for the AEA antigen.SDS‐PAGE of the immunoprecipitate obtained with anti‐AEA serum from radiolabelled glycoprotein fraction of normal lung tissue revealed a single band of 79000 dalton apparent molecular weight.These findings indicate that the AEA constitutes a membrane marker of the type II pneumocytes and might be involved in the secretory process of surfactant. Immunohistological evidence for the presence of AEA in the hyaline membranes of neonatal RDS is also pres

ISSN:0022-3417    

DOI:10.1002/path.1711500408

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711500410

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711500413

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711500401

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY