ISSN:0022-3417    

DOI:10.1002/path.1711580102

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractThe ribosomal genes (located on the acrocentric chromosomes 13–15,21–22) may be identified by their silver stained gene products, i.e. NOR related proteins. The NOR bearing chromosome activity can be observed at metaphase with the potential for all ten chromosomes to be positively stained. On the other hand, during interphase they fuse so that eventually only a single silver positive structure is seen in resting normal cells. Investigations of histopathological sections of non‐Hodgkin's lymphoma (NHL) have demonstrated a correlation between the numbers of interphase NORs and the grade of tumour. There is generally a higher number of interphase in high‐grade, and a lower number in low grade tumours. This histopathological and cytogenetic study of 13 patients with NHL shows that the higher numbers of interphase NORs in the high‐grade tumours is not necessarily a reflection of increased numbers of NOR‐bearing chromosomes. Examples were found of high‐grade neoplasms, showing the expected high numbers of interphase NORs, but not an increased number of NOR‐bearing chromosomes. Conversely, some low‐grade tumours, with the expected low number of interphase NORs, had increased numbers of NOR‐bearing chromosomes.Our conclusion is that the interphase NOR number is related to factors other than chromosome numbers. We suggest that NOR numbers at interphase may be related to cell turnover. This is supported by previous investigations using DNA flow cytometry and the monoc

ISSN:0022-3417    

DOI:10.1002/path.1711580103

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractThe beta chain of the T‐cell antigen receptor is constructed using a variable region gene from one of approximately 20 variable gene families. Antibodies that react with the products of these gene families have been used to demonstrate clonal proliferation of T cells. Here, we describe the expression of two beta‐chain variable region families in normal and reactive lymphoid tissues. In all the tissues studied, expression was scattered throughout the T‐cell areas, without any clustering of expre

ISSN:0022-3417    

DOI:10.1002/path.1711580104

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractRecent studies have suggested that rhabdomyosarcoma can be divided into favourable and unfavourable histology groups. Those subtypes comprising the unfavourable histology group are alveolar, monomorphous round cell and anaplastic rhabdomyosarcoma, with a favourable histology group of embryonal rhabdomyosarcomas. An immunohistological study was undertaken on 59 childhood rhabdomyosarcomas, using antisera to keratin. S100 protein, vimentin, desmin, myoglobin, and troponin T, Our results suggest that desmin is the single most useful antibody in the diagnosis of rhabdomyosarcoma and was expressed in all our cases. The expression of troponin T in the majority of embryonal rhabdomyosarcomas but not in the other histological types has prognostic implications.

ISSN:0022-3417    

DOI:10.1002/path.1711580105

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractA historical series of varioliform gastritis published in 1978 by Lambertet al. (Digestion1978: 17: 159–167) was revisited by two pathologists. The histological preparations from 35 parents were reread and interpreted in the light of endoscopic data found in the files. The results demonstrate a strict correlation between clinical diffuse varioliform gastritis and a recently recognized hispathological entity—lymphocytic gastritis, which is characterized by intense lymphocytic infiltration of the surface and foveolar epithelium. The rare discrepancies occur in Crohn's disease and in varioliform gastritis limited to part of the stomach, mostly the ant

ISSN:0022-3417    

DOI:10.1002/path.1711580106

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractThirty‐two cases of primary liver neoplasms comprising 12 benign, 15 malignant, and 5 cases with equivocal histopathological features between benign and malignant have been investigated using monoclonal antibody (MoAb) Ki 67, which reacts with a nuclear protein expressed in the G1, G2, S, and M phases of the cell cycle.The Ki67 score (positive cells/total neoplastic cells) seems to correlate to the classes of lesions tested. In the hepatocellular carcinoma (HCC) group, the percentage of labelled nuclei, ranging from 15 to 50 per cent, showed a good correlation with Edmondson‐Steiner's histological tumour grade.A percentage of positive cells similar to that of the proved low‐grade HCCs was detected in the five neoplastic lesions in which the routine histopathological criteria of malignancy were not fulfilled. The benign neoplasms showed a very low growth fraction, similar to that of normal or cirrhotic tissues.The use of the Ki 67 score seems to offer useful information about the biological behaviour of some liver masses and may help in the differential diagnosis of hepatocellular adenoma versus carc

ISSN:0022-3417    

DOI:10.1002/path.1711580107

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractA series of cases of high grade non‐Hodgkin's lymphomas has been studied by morphology (1l0 cases), immunocytochemistry (90 cases), using reagents reactive in fixed paraffin‐embedded tissue, and flowcytometry (77 cases). B‐cell tumours constituted 67.0 per cent of the total, T‐cell tumours 22.0 per cent, and unclassified cases 8.8 per cent. Immunocytochemistry revealed two anaplastic carcinomas. Of the 77 cases studied by flow cytometry, 67.5 percent were diploid and 32.5 per cent DNA aneuploid. T‐cell tumours were more likely to be diploid than B‐cell tumours, though the difference did not reach statistical significance, T‐cell tumours had a significantly lower proliferative index (%S + G2) than B‐cell tumours (P= 0.002).The overall remission induction rate was 68 per cent and actuarial 3‐year survival 47 per cent. There was a trend for cases with %S + G  2less than 22 percent to survive longer (P=0.07). This trend became statistically significant when aneuploid cases were added to the high PI group (P = 0.04). No correlation was seen between morphological or immunophenotypic groups and remission induct

ISSN:0022-3417    

DOI:10.1002/path.1711580108

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractTen diffuse pleural mesotheliomas of connective tissue type have been compared with 14 examples of pleural granulation tissue and 7 localized fibrous tumours of the pleura, using immunohistochemistry to identify cytokeralins oflow and high molecular weight and vimentin. Low molecular weight cytokeratin and vimentin were both delected in 8 of the 10 mesotheliomas and in 12 of the 14 reactive iesions. High molecular weight cylokeratin was rarely detected in either lesion. The seven localized fibrous tumours of the pleura were all positive for virnentin and negative for both cytokeratins. These findings support an origin of connective tissue type mesotheliomas from multipotential submesothelial spindle cells and of localized fibrous tumours of the pleura from either conventional fibroblasts or resting submesothelial spindle cells. Antibodies to cytokeratin help distinguish these two neoplasms but provide no assistance in the more difficult diagnostic problem of distinguishing mesotheliomas of connective tissue type from pleural reactions characterized by abundant granulation tissue.

ISSN:0022-3417    

DOI:10.1002/path.1711580109

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractThe appearance of neoplastic human urothelium viewed by low‐temperature scanning electron microscopy (LTSEM) and conventional scanning electron microscopy (CSEM) was compared. Fixed, dehydrated neoplastic cells viewed by CSEM had well‐defined, often raised cell junctions; no intercellular gaps; and varying degrees of pleomorphic surface microvilli. The frozen hydrated material viewed by LTSEM, however, was quite different. The cells had a flat or dimpled surface, but no microvilli. There were labyrinthine lateral processes which interdigitated with those of adjacent cells and outlined large intercellular gaps.The process of fixation and dehydration will inevitably distort cell contours and on theoretical grounds, the images of frozen hydrated material should more closely resemble thein vivoappeara

ISSN:0022-3417    

DOI:10.1002/path.1711580110

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY

摘要:

AbstractMicrosomes isolated from adult human gall‐bladders have for the first time been shown to contain specific glucose‐6‐ phosphatase activity. The gall‐bladder glucose‐6‐phosphatase enzyme has the same molecular weight (36 500 daltons) and similar immunological properties and kinetic characteristics to the hepatic microsomal glucose‐6‐pho

ISSN:0022-3417    

DOI:10.1002/path.1711580111

出版商:John Wiley&Sons, Ltd.    

年代:1989

数据来源: WILEY