摘要:

AbstractThe pathogenesis of silicosis involves interaction between pulmonary macrophages and fibroblasts. The consequences of direct injury to pulmonary cells and the role of inflammatory cells other than the macrophage have received little attention. These were studied over a 20 week period after instilling silica to mice by correlating the changing inflammatory response, as revealed by bronchoalveolar lavage and lung sections, with the cellular location of silica particles and the development and resolution of granulomatous lesions. Within 24 h, a massive concentration of particles and PMN was seen in centrilobular locations with acute focal necrosis of type 1 epithelial cells. Rapid epithelial repair occurred but PMN were recovered from the lung up to 20 weeks. In the alveoli, silica was ingested by PMN and AM, resulting in the death of some cells; free particles crossed the epithelium and were found predominantly in peribronchial macrophages. Silicotic granulomas formed within a week and consisted mainly of fibroblasts macrophages and some PMN. It is suggested that the necrosis of type 1 epithelium and the continuing efflux with serial destruction of PMN may be important factors in the generation of silicotic fibrosis.

ISSN:0022-3417    

DOI:10.1002/path.1711440302

出版商:John Wiley&Sons, Ltd.    

年代:1984

数据来源: WILEY

摘要:

AbstractThe osteosclerosis of idiopathic myeloid metaplasia was investigated by a histodynamic study of undecalcified bone from nine patients. In all cases osteosclerosis resulted from woven bone formation. In moderate osteosclerosis only intrabecular woven bone was observed, with osteoclastic hyperresorption, hyperosteoidosis with many osteoblasts, and an increased calcification rate. This may represent an early stage of the disease, osteosclerosis resulting from increased bone remodelling, with woven bone formation by osteoblasts. However, in extensive osteosclerosis both intratrabecular and medullary woven bone was observed, with moderate osteoclastic hyperresorption, hyperosteoidosis without osteoblasts and a very low calcification rate. The latter pattern could correspond to an advanced stage, and could result from stromal bone formation without osteoblasts. The classical histodynamic profile of osteomalacia was sometimes observed. The link between bone pain and possible osteomalacia remains vague.

ISSN:0022-3417    

DOI:10.1002/path.1711440303

出版商:John Wiley&Sons, Ltd.    

年代:1984

数据来源: WILEY

摘要:

AbstractIn this study we have investigated the mucin profile and the endocrine cell population in gastric endoscopic biopsies from 22 patients affected by chronic gastritis and intestinal metaplasia and in five surgical specimens of stomachs removed because of intestinal‐type carcinoma (4) or peptic ulcer (1). High iron diamine‐Alcian blue (HID‐Ab) staining and peptide immunocytochemistry (peroxidase anti‐peroxidase technique) were used. Forty‐one foci of intestinal metaplasia were detected, 15 produced sulphomucins and 26 sialomucins. Of the endocrine cells investigated, gastrin and somatostatin cells were the most frequently observed, while cholecystokinin, glucose‐dependent insulinotropic peptide‐, secretin‐ and enteroglucagon‐containing cells were also found in the metaplastic areas, but less frequently. No significant correlation was found between the type of mucin and the types of endocrine cells present, the latter usually resembling those normally found in the small intestine. On the basis of these results we conclude that intestinal metaplasia involves mucin‐ and peptide‐producing cells of the stomach in a variable manner, with no correl

ISSN:0022-3417    

DOI:10.1002/path.1711440304

出版商:John Wiley&Sons, Ltd.    

年代:1984

数据来源: WILEY

摘要:

AbstractImposil iron—dextran is an inert tracer that has been used to study mesangial uptake and clearance of macromolecular material from the glomerular circulation. Such a tracer may be a useful marker of altered mesangial function in animals with some forms of glomerulonephritis. We have studied mesangial handling of intravenously injected Imposil (50 mg/100 g body weight) in normal rats by light, immunofluorescence and electron microscopy for up to 3 months. Mesangial cell uptake was maximal at 48–54 h. Extrusion and drainage of tracer to the vascular pole and distal tubule was evident at 3 days but iron was still present in mesangial cells at 3 months. Possible functional renal impairment resulting from persistent mesangially sequestered tracer was examined by measuring daily urine protein and iron excretion. A possible relationship between failure of mesangial cells to eliminate inert tracer and increasing glomerular permeability is demonstrated, suggesting that Imposil and similar inert macromolecules cannot be used for long‐term studies of mesangial fun

ISSN:0022-3417    

DOI:10.1002/path.1711440305

出版商:John Wiley&Sons, Ltd.    

年代:1984

数据来源: WILEY

摘要:

AbstractTwelve cases of malignant spindle‐cell and sarcomatoid tumours of the skin of debatable nature were studied by immunocytochemical methods, using four antisera which might help contribute to resolution of the problems. The initial diagnosis made on structural grounds was confirmed by immunocytochemistry in six of eight cases in which a specific diagnosis had been made (one melanoma, three squamous carcinomas and two atypical fibroxanthomas). One case, initially regarded as AFX was reclassified as a squamous carcinoma, while a further case of possible AFX could not be confirmed by immunocytochemical study. Of the four cases in which structural examination was inconclusive, two were identified as squamous carcinomas and one as a melanoma by virtue of tumour markers. The fourth case was an intriguing actin‐rich tumour of uncertain nature. Immunocytochemistry, despite certain limitations, has a valuable role to play in the analysis of the problematic spindle‐cell malignant and pseudomalignant tumours of the

ISSN:0022-3417    

DOI:10.1002/path.1711440306

出版商:John Wiley&Sons, Ltd.    

年代:1984

数据来源: WILEY

摘要:

AbstractHuman umbilical vein endothelial cells in monolayer culture were used to study the effects of the chemotactic tripeptide,N‐formylmethionylleucylphenylalanine (FMLP), on structure and function of the endothelium. Endothelial cell morphology was unaffected by concentrations of 10−8−10−4M. No effect on endothelial cell proliferative capacity, as measured by the DNA content of cultures, was seen at the FMLP concentrations studied (10−8−10−6M). Using fluorescent molecular probes to investigate FMLP‐induced alterations in membrane structure, it was shown using the monomer–excimer method with pyrene decanoic acid that FMLP caused a marked restructuring of the plasma membrane. This took the form of a restriction of the surface available to the lipophile reporter molecules, probably caused by a molecular reorganization of the membrane protein component. Experiments with diphenylhexatriene indicated that FMLP did not make the plasma membrane of the endothelial cell more fluid. Concomitant with these changes in the physical properties of the membrane, an FMLP‐induced increase in granulocyte adherence to the endothelial cells was observed. A theoretical model is presented correlating granulocyte adherence with the lateral mobility of lipids in the endothelial cell membrane. The significance of the FMLP‐induced increase in granulocyte adherence to endothelial cells for the pathogenesis o

ISSN:0022-3417    

DOI:10.1002/path.1711440307

出版商:John Wiley&Sons, Ltd.    

年代:1984

数据来源: WILEY

摘要:

AbstractHepatic endothelial cells were studied by light and electron microscopy in 48 patients with acute hepatitis due to virus infection or drug idiosyncrasy. Light microscopy revealed cell swelling and appearance of dense refractile intracytoplasmic granules staining with the amylase PAS reaction and for iron by Perls' method. They were orcein‐negative. These cells, regarded as ‘activated’ endothelial cells, were found throughout the parenchyma, especially in the classical form of acute hepatitis. In acute hepatitis with bridging, panacinar or periportal necrosis, activated endothelial cells were prominent in the necrotic areas. They were constantly seen lining newly formed capillaries in these sites. By electron microscopy, the intracytoplasmic granules had the characteristics of primary or secondary siderosomes. In areas of capillarization, basement membrane material was seen on the aspect of the activated cells facing the space of Disse. Activated endothelial cells may play a part in protecting hepatocytes from i

ISSN:0022-3417    

DOI:10.1002/path.1711440308

出版商:John Wiley&Sons, Ltd.    

年代:1984

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711440310

出版商:John Wiley&Sons, Ltd.    

年代:1984

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711440311

出版商:John Wiley&Sons, Ltd.    

年代:1984

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711440312

出版商:John Wiley&Sons, Ltd.    

年代:1984

数据来源: WILEY