ISSN:0022-3417    

DOI:10.1002/path.1711530302

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711530303

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

摘要:

AbstractWe have performed a single blind trial to assess the value of the monoclonal antibodies MB1and MT1in lymphoma classification. Sixty cases of non‐Hodgkin's lymphoma (NHL) were stained with MB1and MT1using an indirect immunoperoxidase technique in paraffin sections. The majority of B tumours (27/33) stained with MB1, and most of the T tumours (24/27) stained with MT1. The MB1antibody often produced rather weak staining but it was apparently highly specific for B cells, with only three (3/27) of the T tumours (two cases of ‘malignant histiocytosis’ of the intestine (MHI) and one pleomorphic T‐cell lymphoma) displaying ‘false’ positivity. The MT1antibody generally produced very strong staining, but it was not very selective, with 14/33 of the B lymphomas displaying ‘false’ positivity.The cross‐reactivity observed in 17 cases led to only three misdiagnoses, two B tumours being designated as T lymphomas and one T tumour being designated as a B lymphoma. In a few cases (7/17), dual staining with both antibodies precluded firm diagnosis. In other cases (6/17), classification was possible despite some of the tumour cells showing dual staining. The seventeenth case was a plasmacytoma displaying MT1positivity only.While the monoclonal antibodies MB1and MT1are of use in classifying lymphomas in paraffin section, they are not entirely lineage‐specific, and the uncritical use of these two reagents alone may give rise to misdiagnosis; the use of a panel of monoclonal antibodies may yield more accurate results. As with any immunohistochemical marker, their limitations should be recognized; interpretation must be judicious and always in the context of the histo

ISSN:0022-3417    

DOI:10.1002/path.1711530304

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

摘要:

AbstractEighteen cases of lymphoplasmacytoid lymphoma (LPL) have been immunophenotypically characterized with a panel of 26 monoclonal antibodies. All cases expressed leucocyte common antigen, class II MHC and stained with B cell markers (CD19, CD20, CD22) although a variable proportion of tumour cells were noticed to have lost some B cell marker expression. There was some phenotypic heterogeneity with variable immunostaining with KB61, CD21, and CD5. A variable proportion of cells in all cases contained cytoplasmic immunoglobulin. Surface immunoglobulin light chain restriction was demonstrated in 11 cases and heavy chain predominance in 16 cases. Few tumour cells were proliferating as indicated by Ki67 immunostaining. A wide variation in number of macrophages and T lymphocytes were present in association with the tumours. A significant association between the expression of CD5 and the presence of peripheral blood lymphocytosis was noticed (p<0·003) but there was no association between CD5 and co‐expression of IgM and IgD. This data supports an origin from non‐germinal centre cells for LPL and suggests that CD5 expression by B cells may be related to lymphocyte migration. LPL shows some immunological heterogeneity and can present diagnostic difficulties, but its poor prognosis makes it an important category of lymphoma to recog

ISSN:0022-3417    

DOI:10.1002/path.1711530305

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

摘要:

AbstractLeu‐M1 is a differentiation antigen present in human myelomonocytic cells. Seventy‐seven acute leukaemias were retrospectively stained with anti‐Leu‐M1 using the immunoperoxidase technique on Bouin‐fixed paraffin‐embedded sections. The subjects were 44 acute lymphoblastic leukaemias (ALL) and 33 acute myeloid leukaemias (AML) previously characterized by cytochemical and immunologic (cell suspension) methods. Leu‐M1 was positive in all the AML and in half of the ALL cases. These results suggest that Leu‐M1 does not allow differentiation between AML and ALL. For the ALL cases Leu‐M1 was positive in 15/28 B‐cell types, 4/12 T‐cell type and 3/4 ‘null’‐cell type cases. Thus, this antibody is of no assistance in defining types B, T, or ‘null’ in ALL. Leu‐M1 was also studied on paraffin sections of 34 high grade malignant lymphomas. The antibody was negative in all 13 B‐cell lymphomas (lymphoblastic: 6; immunoblastic: 7) and in all 4 ‘null’ cell lymphomas. It was positive in 4/9 peripheral T‐cell type, the other T‐cell lymphomas (lymphoblastic: 5; immunoblastic:3) remaining negative. Thus, Leu‐M1 may be positive in T‐cell lymphomas but it is negative in B‐cell lymphomas and is always negative in B or T lymphoblastic types. It seems that lymphoblasts are Leu‐M1 negative in non‐Hodgki

ISSN:0022-3417    

DOI:10.1002/path.1711530306

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

摘要:

AbstractThe cell spectrum of neoplastic and benign reactive germinal centres was determined ultrastructurally. The degree in which the cell composition found in reactive germinal centres is maintained in analogous structures of follicular lymphomas was investigated by pattern recognition methods and discriminant analysis based on the frequencies of the various lymphoid and non‐lymphoid cell types. The follicular lymphomas included lymphomas with predominantly centrocytes (FCCL, Cb‐cc) and lymphomas with predominantly centroblasts (FCCL, Cb).Pattern analysis of FCCL Cb, FCCL Cb‐cc and reactive germinal centres indicates that FCCL Cb follicles resemble reactive germinal centres in more aspects than follicles of FCCL Cb‐cc.Clear statistical differences were encountered between the frequencies of the lymphoid cell types and of the follicular dendritic and histiocytic reticulum cells in follicles of FCCL Cb‐cc and FCCL Cb and reactive germinal centres.Application of a discriminant analysis using a combination of the frequency of centrocytes and follicular dendritic cells demonstrated that both types of neoplastic follicles and reactive germinal centres were correctly classified on the basis of their cell spectrum. For the three groups the most potent discriminator was the centrocyte, whereas the small and large centroblast were of less value. For discrimination between Cb‐cc follicles and reactive germinal centres again the centrocyte was the most potent discriminator. Discrimination between FCCL Cb follicles and reactive germinal centres of FCCL Cb‐cc follicles can be easily achieved using the frequencies of small centroblasts or centrocytes on their own.These findings indicate that (1) follicles of both FCCL Cb and FCCl Cb‐cc differ greatly in the cellular composition and not only with respect to their content of centroblasts but also in their content of follicular dendritic cells and (2) they may be considered as neoplasms representing different developmental phases of g

ISSN:0022-3417    

DOI:10.1002/path.1711530307

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

摘要:

AbstractA study is reported in which anin situhybridization technique for the demonstration of human papillomavirus (HPV) employing a biotin–streptavidin polyalkaline phosphatase complex has been successfully applied to formalin‐fixed paraffin‐processed tissue obtained from ten patients with juvenile laryngeal papillomatosis. In all cases, positive results were obtained for either HPV type 6 or 11. Normal vocal cord epithelium was neg

ISSN:0022-3417    

DOI:10.1002/path.1711530308

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

摘要:

AbstractLung biopsies from four children and two adults with idiopathic pulmonary haemosiderosis have been examined by transmission electron microscopy. No qualitative differences were identified between the children and the adults but the changes were more severe in the children. In each case the major damage involved the capillary endothelium and its basement membrane. Capillary endothelial swelling was very noticeable and in one case the endothelium was attenuated but gaps between endothelial cells were very difficult to find. Capillary narrowing and platelet aggregation were common. The capillary endothelial basement membrane showed focal thickening, particularly on the thick side of the air/blood barrier, but no electron dense deposits were identified. Degenerative changes in the alveolar epithelium were not so marked as those in the capillary endothelium and the epithelial basement membrane was normal except for haemosiderin deposition. Haemosiderin was also noted on elastin and within intra‐alveolar macrophages. Other secondary changes included mild interstitial oedema and fibrosis. These findings indicate that the major site of damage is the alveolar capillary, but provide no evidence of the cause of the diseas

ISSN:0022-3417    

DOI:10.1002/path.1711530309

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

摘要:

AbstractUsing immunocytochemical techniques at the light and electron microscope levels,Legionella pneumophilaand one of its extracellular proteases were located in the lungs of guinea pigs with experimental Legionnaires' disease (LD).L. pneumophilawas immunostained by several peroxidase‐ and gold‐labelling methods for light and electron microscopy. The protease was immunolabelled in tissue fixed in Carnoy's fluid at the light microscopical level and on broth‐grown organisms at the ultrastructural level. It was not labelled in either formalin‐ or glutaraldehyde‐fixed tissue.Using double‐labelling techniques,L. pneumophilaand protease were located in the same section and were shown to be intimately associated with pulmonary lesions, providing strong evidence for the role of this protease in L

ISSN:0022-3417    

DOI:10.1002/path.1711530310

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

摘要:

AbstractImmunocytochemistry has been applied extensively to the diagnosis of intracranial tumours, but meningiomas still present a diagnostic problem. However, desmosomes have been shown by electron microscopy to be present in meningiomas, and this distinguishes them from gliomas. This paper describes a new monoclonal antibody, 11‐5F, against desmosomal proteins 1 and 2 (desmoplakins) and assesses its usefulness in the diagnosis of meningiomas and other intracranial tumours. A total of 74 surgically removed intracranial tumours were examined by fluorescent antibody staining with 11‐5F on frozen sections. In addition, a panel of antibodies against cytokeratin, vimentin, glial fibrillary acidic protein, and S100 protein was used. 11‐5F stained 30/30 meningiomas and 14/14 metastatic carcinomas but 0/30 gliomas, thus distinguishing meningiomas and metastatic carcinomas from gliomas. The distinction between meningiomas and metastatic carcinomas on the basis of intermediate filaments staining was more difficult because neither the anticytokeratin nor the antivimentin antibody was specific for either tumour type. This study emphasizes the value of antidesmosomal antibodies as an important adjunct to the diagnosis of intracranial tu

ISSN:0022-3417    

DOI:10.1002/path.1711530311

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY