ISSN:0022-3417    

DOI:10.1002/path.1711540402

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711540403

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711540404

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711540405

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractModel experiments were designed to assess whether DNA could be recovered from formol–saline fixed peripheral blood lymphocytes and tonsil tissue for use in Southern blot gene analysis. Lymphocytes were fixed for 30 min and tonsil for 6 and 24 h, then paraffin embedded. High molecular weight DNA was extracted by prolonged digestion (2–7 days) with proteinase K or protease XXIV in the presence of 1 per cent sodium dodecyl sulphate. Restriction, transfer and hydridization were possible without modification of standard procedures. Multiple copy sequences were demonstrated using Mspl and Bst Nl restriction and hybridization for the Y chromosome (pHY 2·1 probe), single copy genes using EcoRI and BamHl restriction for the T‐cell receptor β chain (Tβ probe), and Bgl II and Hind III for the immunoglobulin heavy chain (JH probe). Identical banding to unfixed tissue was achieved except when 24 h fixed extracts were used. With these, demonstration of the 24 KB Bam Hl/Tβ and 9·2 KB Hind III/JH bands was not obtained. These findings suggest that as the fixation time is extended, alterations to DNA will limit the available range of restriction enzyme/probe combinations. However, with careful choice of these the extraction of DNA from formalin fixed and paraffin embedded pathological tissue for Southern blotting should be

ISSN:0022-3417    

DOI:10.1002/path.1711540406

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractNormal hyperplastic and malignant endometrial specimens were labelledin vitrowith bromodeoxyuridine (BrdU). S‐phase cells were stained after DNA denaturation, using a monoclonal antibody to BrdU and an indirect immuno‐peroxidase method. Various conditions for BrdU uptake, DNA denaturation, and staining were tested. BrdU labelling was compared with autoradiography using tritiated thymidine, with good correlation. Glandular labelling indices of proliferative endometrium were significantly higher than both secretory and hyperplastic endometrium but were similar to carcinoma. Stromal labelling showed the same trend but the differences were not statistically signific

ISSN:0022-3417    

DOI:10.1002/path.1711540407

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractHuman pregnancy‐associated α2‐glycoprotein (α2‐PAG) is a high molecular weight glycoprotein present in normal sera. The protein is present in high concentration in the sera of pregnant females and in abnormally low concentration in association with conditions connected with abnormalities of mucosal immunity.Indirect immunoperoxidase techniques using poly‐ and monoclonal antibodies were employed to identify pregnancy‐associated α2‐PAG in different tissues. Four monoclonal antibodies were selected from a battery of antibodies with defined specificities in order to ascertain reactivity with various epitopes of the antigen. The antibodies were applied to paraffin sections of breast, colon, salivary gland, and tonsil, and different fixation regimes were used in the preparation of the tissues. The polyclonal antibodies were found to stain plasma cells and epithelial lumina evenly in all the tissues included whereas the monoclonal antibodies were shown to stain certain components selectively. In breast and salivary glands, all four monoclonal antibodies could identify α2‐PAG, but in tonsil and colon, only two were reactive. This difference in epitope expression might reflect the internal processing of α2‐PAG, and lack of availability of certain epitopes may be indicative of functional blockin

ISSN:0022-3417    

DOI:10.1002/path.1711540408

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractPrevious studies have shown that 80–90 per cent of cases of atypical hyperplasia of the endometrium do not progress to cancer. Criteria to predict the outcome in an individual patient with hyperplasia are lacking, and hysterectomy is the usual (over)treatment in order to avoid a 10–20 per cent chance of confrontation with cancer later on in the course of the disease. A recent study has shown that using a nuclear morphometric classification rule, 15 per cent of patients without progression can be accurately separated from patients with progression. However, as it is unlikely that nuclear morphometrical features are the only morphological factors reflecting the outcome of the disease, other quantitative parameters describing the architecture of the glands have also been studied for their potential value in selecting patients who will progress to cancer. In total, 10 nuclear features and 12 glandular architectural features were studied in 39 cases of atypical endometrial hyperplasia. Among these cases, seven (18 per cent) progressed to cancer.Using linear stepwise regression analysis and discriminant analysis, the volume percentage stroma and the standard deviation of the shortest nuclear axis are the best discriminators, although the outer surface density of the glands also adds to the discriminating power. The volume percentage stroma is the best single prognosticator; this feature is highly reproducible. In total, using these combined architectural and nuclear morphometrical features, 20 of the 32 cases without progression were separated from those who subsequently progressed (62·5 per cent). This is a considerable improvement over nuclear morphometrical features alone (15 per cent separa

ISSN:0022-3417    

DOI:10.1002/path.1711540409

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractA gland‐like cellular proliferation was observed in the deepest portion of the rete testis in both testes from a 55‐year‐old man who died from metastasis of breast carcinoma to the brain. The gland‐like proliferations consisted of columnar epithelial cells supported by a thin lamina propria. These cells displayed irregularly outlined nuclei with deep indentations. Atypias and mitoses were infrequent. The term ‘adenomatous hyperplasia of the rete testis’ is proposed to designate the benign lesion re

ISSN:0022-3417    

DOI:10.1002/path.1711540410

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY

摘要:

AbstractA study has been made of mast cells (MC) in surgically resected appendices using a long toluidine blue (LTB) staining method. The numbers of MC in measured areas of both mucosa and submucosa/muscularis were counted and comparisons made between 22 appendices containing threadworms and 22 which were histologically normal. There was considerable variation in MC numbers from case to case and the patients aged under 15 as a group had a higher mean number of mucosal MC than the older patients. The reasons for the high individual variation could not be identified from the histological sections, and no correlation was found between MC numbers and the presence of threadworms.

ISSN:0022-3417    

DOI:10.1002/path.1711540411

出版商:John Wiley&Sons, Ltd.    

年代:1988

数据来源: WILEY