ISSN:0022-3417    

DOI:10.1002/path.1711720302

出版商:John Wiley&Sons, Ltd.    

年代:1994

数据来源: WILEY

摘要:

AbstractRecent evidence from genetic studies suggests that abnormalities of some of the members of the cyclin superfamily may be intimately associated with tumourigenesis, most likely through deregulation of the cell cycle control. In an attempt to elucidate the potential role of cyclin D1 (a gene located within the 11q13 amplicon and a candidate BCL‐1, PRAD‐1 oncogene) in the pathogenesis of human neoplasias, we have developed and characterized a novel monoclonal antibody specifically recognizing cyclin D1 protein in various assays including immunohistochemistry on frozen and paraffin sections. Using the DCS‐6 antibody as a tool, we now show a characteristic cell cycle‐dependent variation of the cyclin D1 protein in human cultured cells and report on the first immunohistochemical study of this G1 cyclin in a range of normal human tissues and breast carcinomas. Analysis of normal tissues revealed generally low levels of cyclin D1 protein, mainly restricted to the proliferative zones of some epithelial tissues, and the lack of its expression in several human tissues including lymph nodes, spleen, and tonsils. In contrast, pronounced overexpression/nuclear accumulation of cyclin D1 was found in 37 per cent of cases in a series of 35 primary ductal carcinomas of the breast. We conclude that the DCS‐6 antibody provides a potentially useful tool for the establishment of simple methods suitable for verifying any diagnostic and/or prognostic value of this novel marker on large series of histological specimens and opens the way for biochemical, immunocytochemical, and immunohistochemical studies of the role played by cyclin D1 aberrations in human on

ISSN:0022-3417    

DOI:10.1002/path.1711720303

出版商:John Wiley&Sons, Ltd.    

年代:1994

数据来源: WILEY

摘要:

AbstractIn order to assess its discriminating and prognostic value, we studied immunoreactivity for proliferating nuclear cell antigen (PCNA) in human malignant mesothelioma (31 cases) and in human non‐neoplastic mesothelium (33 cases with reactive mesothelium and 20 cases of normal mesothellum) using the murine monoclonal antibody PC 10. We also compared it with mitosis counts expressed as the mitotic volume index (MV index). There were differences between malignant mesothelioma, reactive mesothelium, and normal mesothelium for percentage of PCNA immunoreactive cells (mean ± SD; 27 ± 9, 9·5 ± 5·1, and 3·6 ± 1·6, respectively) and for their MV index (20·3 ± 4·5, 9·4 ± 2·1, and 3·6 ± 0·6, respectively). The median actuarial survival was 10·1 months for patients with less than 25 per cent PCNA immunoreactive cells, 9·4 months for patients with less than 20 mitoses per mm2of tumoural tissue, 5·9 months for patients with more than 25 per cent PCNA immunoreactive cells, and 5·3 months for patients with more than 20 mitoses per mm2of tumoural tissue. Our results suggest that PCNA immunoreactivity is useful in discriminating between neoplastic and non‐neoplastic mesothelium and that it may have prognostic value i

ISSN:0022-3417    

DOI:10.1002/path.1711720304

出版商:John Wiley&Sons, Ltd.    

年代:1994

数据来源: WILEY

摘要:

AbstractThe histological localization of theretproto‐oncogene (proto‐ret) product was examined in neural crest‐derived and neuronal tissues together with their neoplastic counterparts by immunohistochemistry using a polyclonal antibody. Schwann cells, neurons, sympathetic ganglia, and cells of the adrenal medulla were positive for the proto‐retproduct, whereas melanocytes were negative. Positive results were obtained from neural crest‐derived tumours such as schwannoma (69 per cent, 11/16), neurofibroma (59 per cent, 13/22), neuroblastoma (80 per cent, 4/5), phaeochromocytoma (100 per cent, 3/3) and medullary thyroid carcinoma (100 per cent, 3/3). The antibody reacted with all of the 22 astrocytomas examined. With negative proto‐retexpression in melanocytic tumours, proto‐retexpression was considered to correlate with the differentiation of some lineages of neural crest

ISSN:0022-3417    

DOI:10.1002/path.1711720305

出版商:John Wiley&Sons, Ltd.    

年代:1994

数据来源: WILEY

摘要:

AbstractNm23 is a putative metastasis suppressor gene and alterations in this gene have been reported in colorectal carcinomas suggestive of a role for nm23 in the dissemination of these tumours. In this study we used an affinity purified polyclonal antibody, ab‐11, on formalin‐fixed, paraffin‐embedded sections of colorectai carcinomas from 46 patients in a three‐stage avidin–biotin complex immunoperoxidase technique. Follow‐up of these patients was until time of death or for 5 years, with a mean time of 31·2 months. Two observers scored the staining results from 1 to 3 according to the proportion of tumour cells positive. The association of nm23 staining with survival, sex, age, vascular invasion, and Dukes' stage was determined using Cox's regression model. The association of death from colorectal cancer and nm23 status reached marginal significance in this study (P= 0.0417). Moreover, there is some suggestion of a protective effect from nm23 as the relative risk of dying from colorectal cancer for each increment of nm23 positivity is 0·573 (95 per cent confidence limi

ISSN:0022-3417    

DOI:10.1002/path.1711720306

出版商:John Wiley&Sons, Ltd.    

年代:1994

数据来源: WILEY

摘要:

AbstractOne hundred and thirty‐one thyroid tumours were examined immunohistochemically for expression of retinoblastoma (RB) and p53 protein. The results demonstrate that RB protein is not lost in any cases, indicating that inactivation of the RB gene is unlikely to play a central role in the pathogenesis of thyroid tumours. Eighteen of 24 (75 per cent) undifferentiated carcinomas, 6 of 32 (19 per cent) papillary carcinomas, 5 of 29 (17 per cent) follicular carcinomas, and 6 of 46 (13 per cent) medullary carcinomas showed p53 protein nuclear staining. In 46 per cent of the undifferentiated carcinomas many of the tumour cells had accumulated p53 protein, whereas in the other positive cases less than 5 per cent of the cells had increased p53 protein levels. Our results strongly suggest that p53 protein abnormalities play a crucial role in the progression of well‐differentiated to undifferentiated thyroid carcino

ISSN:0022-3417    

DOI:10.1002/path.1711720307

出版商:John Wiley&Sons, Ltd.    

年代:1994

数据来源: WILEY

摘要:

AbstractThe expression ofbcl‐2 is associated with inhibition of apoptosis and prolonged cell survival. The purpose of this study was to examine the immunoreactivity of neuroblastoma and ganglioneuroblastoma tissue samples to thebcl‐2gene product in order to see if it was related to prognosis. BCL‐2 protein was detected in all the 46 formalin‐fixed, paraffin‐embedded samples from 34 patients representing all clinical stages and sites of involvement. Immuno‐positivity was observed in tumours from the primary and metastatic sites. Moreover, it was demonstrated in the pre‐chemotherapy and the post‐chemotherapy samples from six cases with stage 4 disease. It was observed in neuroblasts in various stages of differentiation. A small proportion of undifferentiated neuroblasts were negative. As BCL‐2 oncoprotein was present in all the cases irrespective of the clinical outcome, it does not appear to be one of the factors infl

ISSN:0022-3417    

DOI:10.1002/path.1711720308

出版商:John Wiley&Sons, Ltd.    

年代:1994

数据来源: WILEY

摘要:

AbstractLittle attention has been paid to the composition of the extracellular matrix in synovial sarcoma, a tumour showing both epithelial and mesenchymal phenotypes. As extracellular matrix participates actively in interactions between epithelial and mesenchymal tissues, further knowledge of the pathogenesis of this tumour may be provided by the study of extracellular matrix components. Therefore, we have analysed the immunohistochemical distribution of type I, III, and IV collagen, fibronectin, laminin and tenascin in four cases of synovial sarcoma. The pattern of immunoreactivity for these molecules varied according to the tissue phenotype of the tumour. Mesenchymal tissue labelled mainly for type I and III interstitial collagen and fibronectin. The epithelial component was surrounded by a laminin and type IV collagen‐positive basement membrane, but punctate pericellular reactivity for laminin and type IV collagen was also detected among some mesenchymal cells. Tenascin was strongly expressed in the mesenchymal tissue immediately around epithelial structures and weakly or not at all expressed in the monophasic tumours and in mesenchymal tissue distant from epithelial elements in the biphasic tumours. These results suggest some resemblances between synovial sarcoma and the embryonic development of epithelia from mesenchymal cells, providing further support for the concept of an epitheliogenesis from the mesenchyme in these tumour

ISSN:0022-3417    

DOI:10.1002/path.1711720309

出版商:John Wiley&Sons, Ltd.    

年代:1994

数据来源: WILEY

摘要:

AbstractA detailed temporal sequence of vessel wall changes in a rabbit model of balloon angioplasty, to the iliac arteries, at 30 min, 2h, day 1, day 3, day 7, day 14, 1 month, and 3 months was established using light microscopy, immunostaining for proliferating cell nuclear antigen, and electron microscopy. Complete endothelial denudation with extensive platelet and fibrin deposition was evident early. Intimal thickening, characterized by myointimal hyperplasia, was observed at day 7 and reached a maximum at 1 month. Re‐endothelization was complete by day 14. Cellular proliferation, seen early at day 1, reached a maximum at day 7 for the media and day 14 for the intima before returning to baseline levels by 1 month. This simple model of angioplasty with its reproducible myointimal hyperplasia should prove useful for assessing any potential agents for inhibiting this proces

ISSN:0022-3417    

DOI:10.1002/path.1711720310

出版商:John Wiley&Sons, Ltd.    

年代:1994

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711720311

出版商:John Wiley&Sons, Ltd.    

年代:1994

数据来源: WILEY