ISSN:0022-3417    

DOI:10.1002/path.1711530402

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711530403

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

ISSN:0022-3417    

DOI:10.1002/path.1711530404

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

摘要:

AbstractThe elastosis of 11 invasive ductal and infiltrative lobular carcinomas of the breast was specifically immunostained for the plasma protease inhibitors alpha‐1 antitrypsin, alpha‐1 antichymotrypsin, alpha‐2 macroglobulin, inter alpha trypsin inhibitor and C1 esterase inhibitor. None of these components was detected in the elastic fibres of normal ducts or blood vessels in the breast. The elastosis in breast carcinomas was also stained by Concanavalin A andTriticum vulgarislectins. Such lectin staining probably represents binding to the microfibrillar component of elastic fibres, which is increased in immature elastic fibres, thus suggesting that the elastotic fibres of breast carcinoma are recently synthesised. It is suggested that the presence of protease inhibitors may influence the metabolism of elastic fibres, facilitating elastic fibre proliferation by the inhibition of elastinolytic en

ISSN:0022-3417    

DOI:10.1002/path.1711530405

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

摘要:

AbstractIt has been proposed that intense immunohistochemical staining using the monoclonal antibody NCRC 11 is indicative of a better prognosis in breast cancer, and that expression of pregnancy specific glycoprotein (SP1) and epidermal growth factor receptors (EGFR) are poor prognosis indicators. In order to evaluate the significance of staining for NCRC 1 1 to prognosis and to these other variables, we studied two series of breast cancer patients. In one series (n= 93), staining with NCRC 11 and for SP1 was correlated with prognosis: in neither case was there any overall relationship between staining and survival. However, when the patients were stratified by tumour histological (Bloom's) grade, SP1 expression was associated with a poorer prognosis in grade II tumours (p<0·025). In a further series (n= 94), NCRC 11 staining was carried out and fresh tumour samples were assayed biochemically for oestrogen receptor (OER) and EGFR. NCRC 11 showed a negative correlation with EGFR status (p=<0·05) but no significant association with OER status. Thus despite our demonstration of a negative correlation with an indicator of poorer prognosis, we were unable to demonstrate any direct relationship between staining with NCRC 11 and surviva

ISSN:0022-3417    

DOI:10.1002/path.1711530406

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

摘要:

AbstractRat parotid gland atrophy after unilateral duct ligation was studied by light and electron microscopy. Death of secretory acinar cells, which took the form of apoptosis, resulted in their complete disappearance within 5 days. The remnants of the dying cells were mostly phagocytosed and degraded by macrophages within the glandular epithelium; a few were taken up by adjoining epithelial cells. The acinar cell deletion was accompanied by increased mitosis of striated and intercalated duct epithelial cells. However, over many weeks, there was enhanced apoptosis of duct cells, which eventually led to marked shortening of intercalated ducts. Apoptosis of capillary endothelial cells was observed and may account for the reduction in the capillary bed known to accompany gland atrophy. The end‐stage lesion comprised small numbers of ducts in a condensed stroma. Compensatory hyperplasia, involving proliferation of duct and acinar cells, was demonstrated in the contralateral gland

ISSN:0022-3417    

DOI:10.1002/path.1711530407

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

摘要:

AbstractThe morphology and frequency of the various types of cell death occurring spontaneously in a mouse mastocytoma growing as intramuscular nodules were investigated. In addition to necrosis and apoptosis, which have been well documented in malignant neoplasms in the past, a third morphological pattern of cell death, the formation of dark cells, was observed. Necrosis first appeared in confluent patches about 5 days after tumour inoculation, and these increased in size as the tumours grew. Apoptosis, on the other hand, was present at all stages of tumour growth, and remained at a reasonably constant level. Dark cells were first observed at about 6 days, and increased in numbers thereafter. Dark cells were characterised by overall cellular condensation and gross swelling of mitochondria. Their cytoplasm became squeezed out between adjacent cells and then fragmented. Remnants of dark cells were eventually phagocytosed and degraded by macrophages. The distribution of dark cells in the tumours suggested that crowding and compression may contribute to their development.

ISSN:0022-3417    

DOI:10.1002/path.1711530408

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

摘要:

AbstractThe incidence of appendicitis shows a marked variation between populations which has been attributed to dietary differences. Neural mechanisms and serotonin discharge from subepithelial neurosecretory cells have been previously implicated in pain referable to the appendix and appendicitis. Forty consecutive appendicectomy specimens from Malawi were studied by staining with haematoxylin and eosin, an alcian blue – PAS diastase sequence coupled with lead haematoxylin (PbH) and immunohistology for serotonin and NSE. The findings were compared with those in appendices removed at the Middlesex Hospital, London, to see if there were any differences between a population with a low risk of appendicitis (Malawi) and a high risk population (England). Acute transmural appendicitis was seen in fewer appendices from Malawi (27·5 per cent) than in English appendices (58 per cent). Subepithelial neurosecretory cells identified with PbH were present in 20 per cent of appendices from Malawi and 69 per cent of English appendices. These cells in both series showed immunohistochemical staining for serotonin. Nerve hyperplasia identified by staining for NSE in the appendix was present in 17·5 per cent and 81 per cent of non‐inflamed appendices from Malawi and England respectively.Appearance of subepithelial neurosecretory cells and hyperplasia appear to be concomitants of an increased risk of appendicitis. Neural mechanisms may participate in adapting to a low residue diet and in some cases may generate appendi

ISSN:0022-3417    

DOI:10.1002/path.1711530409

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

摘要:

AbstractDesmosomes are intercellular adhesive junctions that occur in almost all epithelia and should therefore be useful as epithelial markers in tumour diagnosis. Here, we describe a monoclonal antibody, 32‐2B, to a major desmosomal glycoprotein (dg1) which reacts with human tissues in paraffin sections. This antibody was tested for its ability to stain epithelia and tumours. It reacted with all epithelia tested and with every specimen of a wide range of carcinomas. It also stained meningiomas, another desmosome‐containing tumour. It did not stain other types of tumours including lymphomas, melanomas, and various sarcomas, or normal tissues which lack desmosomes.These characteristics demonstrate that 32‐2B is a reliable epithelial marker that may have a useful role in diagnostic histopath

ISSN:0022-3417    

DOI:10.1002/path.1711530410

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY

摘要:

AbstractProduction of a new monoclonal antibody designated NCL‐5D3 is described. The antibody recognizes several low molecular weight cytokeratins, in particular cytokeratin Moll number 8 as determined by immunoblotting studies, and is highly effective for immunocytochemistry using routinely processed paraffin‐embedded material. Staining is enhanced by prior treatment of the sections with trypsin. Assessment using a wide variety of normal and neoplastic tissue indicates reactivity with all tissues of simple or glandular epithelial origin, and in addition with many squamous carcinomas. Thus the antibody should prove of value in diagnostic histopathol

ISSN:0022-3417    

DOI:10.1002/path.1711530411

出版商:John Wiley&Sons, Ltd.    

年代:1987

数据来源: WILEY