ISSN:0012-2823    

DOI:10.1159/000201520

出版商:S. Karger AG    

年代:1997

数据来源: Karger

ISSN:0012-2823    

DOI:10.1159/000201521

出版商:S. Karger AG    

年代:1997

数据来源: Karger

摘要:

Sucralfate, which binds to the matrix of the ulcer bed, is theoretically advantageous for duodenal ulcer therapy, but has not fulfilled its promise clinically. We examined the effects of sucralfate and related compounds in a human intestinal epithelial (Caco-2) cell culture model of restitution on sheet migration across and adhesion to collagen I. Migration was quantitated across a collagen I matrix treated with sucralfate or related compounds and correlated with cell adhesion. Caco-2 motility was significantly and dose-responsively inhibited by sucralfate at therapeutic luminal concentrations. Sucrose octaacetate, the sucralfate backbone, and lactose octaacetate exhibited similar effects while the β-bonded disaccharide maltose octaacetate had little effect. Sucrose itself slightly stimulated motility. Adhesion effects paralleled motility. Thus, sucralfate may inhibit intestinal epithelial motility by sterically interfering with adhesion to collagen I. A sucralfate analog with a lactose octaacetate backbone might retain growth factor binding without inhibiting enterocyte motility, perhaps improving its clinical efficacy

ISSN:0012-2823    

DOI:10.1159/000201421

出版商:S. Karger AG    

年代:1997

数据来源: Karger

ISSN:0012-2823    

DOI:10.1159/000201522

出版商:S. Karger AG    

年代:1997

数据来源: Karger

ISSN:0012-2823    

DOI:10.1159/000201523

出版商:S. Karger AG    

年代:1997

数据来源: Karger

ISSN:0012-2823    

DOI:10.1159/000201524

出版商:S. Karger AG    

年代:1997

数据来源: Karger

摘要:

The risk of developing gallstones is increased in obese subjects. We have investigated whether gallbladder motility in obese subjects is different from that in lean control subjects. In 25 healthy non-diabetic obese subjects and 20 age-and sex-matched lean controls, fasting gallbladder volumes, gallbladder contraction in response to cephalic vagal cholinergic stimulation by modified sham feeding (MSF) and to hormonal stimulation with cholecystokinin (CCK) were studied. Gallbladder volumes were measured during a 30-min MSF period followed 1 h later by a 1-hour continuous i.v. infusion of 0.5 IDU/kg ideal weight of CCK-33. Fasting gallbladder volumes were significantly (p < 0.001) larger in obese (47 ± 4 cm3) compared to lean subjects (24 ± 2 cm3). Fasting gallbladder volume was correlated with body mass index (p < 0.01). Gallbladder contraction during MSF was significantly (p < 0.01) reduced in obese (12 ± 2%) compared to lean subjects (22 ± 3%). CCK infusion, leading to physiological post-prandial plasma CCK levels, induced a significantly (p < 0.001) greater absolute gallbladder contraction in obese (27 ± 3 cm3) compared to lean subjects (15 ± 1 cm3) but the percentage gallbladder contraction was in the same range (64 ± 3% vs. 67 ± 4%, respectively). In addition, residual gallbladder volumes after CCK infusion were significantly (p < 0.001) larger in obese (15 ± 2 cm3) than in lean subjects (7 ± 1 cm3 40 cm3) and one with normal (≤40 cm3) fasting gallbladder volumes. Only obese subjects with increased fasting volumes showed abnormal gallbladd

ISSN:0012-2823    

DOI:10.1159/000201422

出版商:S. Karger AG    

年代:1997

数据来源: Karger

ISSN:0012-2823    

DOI:10.1159/000201525

出版商:S. Karger AG    

年代:1997

数据来源: Karger

ISSN:0012-2823    

DOI:10.1159/000201526

出版商:S. Karger AG    

年代:1997

数据来源: Karger

ISSN:0012-2823    

DOI:10.1159/000201527

出版商:S. Karger AG    

年代:1997

数据来源: Karger