摘要:

In the present study, sera from chronic hepatitis B surface antigen (HBsAg) carriers positive for antibody to hepatitis B ‘e’ antigen (anti-HBe) with evolutive liver disease as correlated with anti-HBe-positive healthy carriers, were examined for antibodies to hepatitis C virus (HCV). Anti-HCV antibodies were detected in 32/124 (25,8%) anti-HBe-positive carriers with chronic liver disease and in none of the 46 healthy carriers. When anti-HCV positivity was evaluated in relationship to the degree of severity of liver disease and possible confounding factors such as hepatitis B virus replication or other potential hepatolesive factors were eliminated by using logistic regression, the odds ratio of liver cirrhosis versus chronic persistent hepatitis was 18 (95%, CI 3.5–92.5). Therefore, our results indicate that HCV may be implicated in the determinism and severity of liver damage in a significant proportion of anti-HBe-positive chronic HBsAg car

ISSN:0012-2823    

DOI:10.1159/000200751

出版商:S. Karger AG    

年代:1991

数据来源: Karger

摘要:

In an immunohistopathological study, we have used the specific monoclonal antibody myc 1–9E10 to the c-myc oncoprotein in 88 gastric carcinomas (22 gastric biopsies and 66 gastrectomies for cancer). Positive myc p62 immunoreactivity was shown in 48 (55 %) cases with moderate or intense staining. The remaining 40 cases exhibited negative or equivocal staining. Normal stomach mucosa was generally nonreactive, with the exception of parietal cells. Elevated c-myc expression was not found to correlate with histological differentiation or in patients with metastases in one or more perigastric lymph nodes. A correlation was found between the level of c-myc expression and the stage of the disease, (p = 0.04); positive c-myc staining was found in 0/4 early gastric cancers and in 48/84 with advanced disease. Also, an association was found between the elevated c-myc expression and depth of invasion (p = 0.1; 0/4 mucosa and submucosa, 2/6 muscularis propria and 25/47 serosa). The c-myc monoclonal myc 1–9E10 may therefore be of use as a marker of advanced disease and depth of invasion in stomach can

ISSN:0012-2823    

DOI:10.1159/000200752

出版商:S. Karger AG    

年代:1991

数据来源: Karger

摘要:

Activation of enzyme phospholipase A2 (EC 3.1.1.4), the major liberator of arachidonic acid, has been proposed as a component in the pathogenesis of inflammatory bowel disease. To test the hypothesis that phospholipase A2 activity is increased in ulcerative colitis, enzyme activity was investigated in colonic biopsies using radiolabelled Escherichia coli as substrate. The study comprised patients with ulcerative colitis (n = 19) and controls without inflammatory bowel disease (n = 7). Ulcerative colitis patients were grouped into those with active disease (n = 8) and those in remission (n = 11) at the time of colonoscopy. No differences were found in mucosal phospholipase A2 activity between patients with active disease, patients in remission and controls. With the present assay we were unable to demonstrate activation of phospholipase A2 as a mechanism in the inflammatory process of ulcerative colitis.

ISSN:0012-2823    

DOI:10.1159/000200753

出版商:S. Karger AG    

年代:1991

数据来源: Karger

摘要:

The alteration of CCK receptor binding on the pancreatic acini in vitro following the induction of pancreatitis was investigated in male rats. Pancreatitis was induced by administering 5 intraperitoneal injections of caerulein, 40 μg/kg each at hourly intervals. The uptake of [3H]-thymidine in the pancreatic acini increased on day 7 following caerulein administration. The release of amylase stimulated by CCK-8, and CCK receptor analysis using bioactive [125I]-BH-CCK-8, were performed at regeneration stage, on days 14 and 28 following the injections. The maximal release of amylase stimulated by CCK-8 was reduced on day 14 by about 40% and recovered on day 28. On day 14 there was a decreaes of 60% in the number of high-affinity receptors and an increase of 161 % in the number of low-affinity receptors. On day 28 there was a 128% increase in the number of low-affinity receptors. Accordingly, we suggest that the CCK receptors of the regenerating cells following caerulein-induced pancreatitis differ from those of the intact cells

ISSN:0012-2823    

DOI:10.1159/000200754

出版商:S. Karger AG    

年代:1991

数据来源: Karger

ISSN:0012-2823    

DOI:10.1159/000196267

出版商:S. Karger AG    

年代:1931

数据来源: Karger

摘要:

The fecal excretion of 111In -oxine-labeled autologous granulocytes was determined in 58 patients with Crohn’s disease. A representative analysis of the total amount of excreted cells requires a 4-day stool sampling at least in those patients suffering from Crohn’s ileitis or ileocolitis. Various laboratory tests (Orosomucoid, α1-antitrypsin, C-reactive protein, erythrocyte sedimentation rate, leukocytes, thrombocytes, albumin, Fe, α1-globulin and α2-globulin) and the van Hees index significantly correlated with this specific estimate of intestinal inflammation, indicating that it is of little importance which one is clinically used for the assessment of inflammatory activity in Crohn’s

ISSN:0012-2823    

DOI:10.1159/000200755

出版商:S. Karger AG    

年代:1991

数据来源: Karger

摘要:

Antibodies to the preS2 antigen (anti-preS2) of the hepatitis B virus (HBV), including IgA, IgM and IgG classes, were observed in patients with acute and persistent HBV infection. In acute HBV infection, rapid and marked serum IgM and IgA anti-preS2 responses were observed. Antibodies reached a peak of serum activity at about 1–2 months after the onset of clinical symptoms, and both antibodies disappeared from serum at 4 months after. IgG anti-preS2 was detected in the early phase of the illness, then the level of IgG anti-preS2 gradually rose during the recovery phase. In persistent HBV infection, IgG and IgM anti-preS2 were detected in sera where the preS2 antigen was present, and IgM anti-preS2 was significantly higher (p < 0.05) in HBeAg-positive than in HBeAg-negative patients. These results indicate that an adequate humoral immune response to the preS2 antigen is induced during acute and persistent HBV infectio

ISSN:0012-2823    

DOI:10.1159/000200756

出版商:S. Karger AG    

年代:1991

数据来源: Karger

ISSN:0012-2823    

DOI:10.1159/000196268

出版商:S. Karger AG    

年代:1931

数据来源: Karger

摘要:

Serum concentrations of primary bile acids were determined at different disease activities in juvenile ulcerative colitis and in healthy age-matched controls. In patients with ulcerative colitis in clinical remission, serum levels of bile acids were also studied after long-term intake (6 months) of dietary fibers (wheat fiber and ispaghula, respectively) in a double-blind randomized cross-over study. Blood samples were taken in the morning after an overnight fasting and for 4 h postprandially after a standardized test meal. Determinations of bile acids were made by radioimmunoassays. Patients with total colitis in the active phase had significantly higher serum levels of cholic and chenodeoxycholic acids 4 h postprandially compared with control children. After long-term intake of ispaghula, significantly higher (although not different from controls) serum levels of cholic acid were found 2 and 3 h postprandially, whereas wheat fibers did not affect serum bile acid concentrations. These results may suggest an increased absorption of unconjugated bile acids in the diseased colon and a minimal influence of dietary fibers on serum bile acid concentrations.

ISSN:0012-2823    

DOI:10.1159/000200757

出版商:S. Karger AG    

年代:1991

数据来源: Karger

摘要:

Little information is available on the role of inflammation and small vessel alteration in the development and healing of peptic ulcers. We studied the spontaneous healing of experimental ulcers in the rat stomach with a novel radio-isotope technique for the monitoring of capillary damage in ulcer-related inflammation. Following ulcer induction, healing was assessed on days 1 7 and 15 by macroscopical and histological determinations of ulcer size and lesion area, parietal cell counts in the ulcer margin and nuclear imaging of the lesion area using Nanocoll®, a 99mTc-labelled colloid. The ulcers were re-epithelized after 15 days. However, Nonocoll activity was still significantly enhanced in the ulcer area, and regression of the microscopically visible lesion area, which, from day 1 on, occupied a zone considerably larger than the ulcer itself, was delayed and still incomplete at the end of the study. Compared to the intact mucosa, parietal cells were reduced in the scar tissue up to 15 days (24–40%). However, despite macroscopical and histological re-epithelization, small vessel leakage and substantial tissue alteration persisted in the ulcer sc

ISSN:0012-2823    

DOI:10.1159/000200758

出版商:S. Karger AG    

年代:1991

数据来源: Karger