ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1982.tb00830.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACT—Factors influencing the preservation of HBcAg in formalin‐fixed, paraffin‐embedded liver tissue have been analysed. Fixation time, choice of the embedding medium, deparaffinization and selection of anti‐HBc proved to be critical variables. For optimal results, fixation in 4% buffered formalin for no longer than 24–48 h, the use of wax with a low melting point, a heating step prior to deparaffinization, and a search for anti‐HBc specifically reactive for paraffinated HBcAg are

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1982.tb00831.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACT—Using immunofluorescence and double‐labelled antisera L and M pyruvate kinase (PK), we show that PK is localized in hepatocyte cytoplasm. The response is modulated by different dietary and hormonal conditions.In physiological conditions, only L PK is noted: slight with normal diet (or 35μg of glucagon) and non‐existent with starvation (or 350 μg of glucagon). L PK fluorescence is maximal after a carbohydrate‐rich diet.In experimental or genetic(Zucker rat)hyperinsulinemia, hepatocytes are both L PK and M PK positive.After partial hepatectomy, this double specificity is found between 48 h and the 7th day. The decrease of PK fluorescence after starvation or glucagon concerns the L PK form of the enzyme. With exo‐ or endogenous insulin, the increase of PK (as insulinemia assays from Zucker rats or after partial hepatectomy confirm) is essentially due to the presence of M PK in

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1982.tb00832.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACT—Two of three patients with infantile polycystic disease and all three patients with congenital hepatic fibrosis revealed multiple gross cystic dilatation of the intrahepatic biliary tree, referred to as Caroli's disease. All three patients with congenital hepatic fibrosis showed recurrent cholangitis related to coexisting Caroli's disease, and two of them died of sepsis following cholangitis. There were several common morphologic findings in the intrahepatic biliary tree of macroscopic and microscopic levels in infantile polycystic disease and congenital hepatic fibrosis with Caroli's disease: 1. irregular, non‐obstructive dilatation of the duct lumen; 2. bulbar protrusion of the duct wall into the lumen; and 3. bridge formation of the duct wall across the lumen. These ductal changes might be caused by a combination of uneven and disproportionate overgrowth of biliary epithelia and their supporting connective tissue. This pathogenetic mechanism might be operative along the entire intrahepatic biliary system in this disease group, and involvement of the smaller levels in early life might result in infantile polycystic disease and congenital hepatic fibrosis and simultaneous or possibly later involvement of the larger levels in Caroli's dise

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1982.tb00833.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACT—A case of chronic non‐A, non‐B (NANB) hepatitis in a 17‐year‐old male is reported. The special feature was microtubular aggregates described so far only in experimental NANB hepatitis in chimpanzees. Additional characteristics were a severe chronic active hepatitis with confluent necrosis in a late stage, multinucleated hepatocytic giant cells and nuclear aggregates of 20–27 nm viruslik

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1982.tb00834.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACT—Intracellular, eosinophilic, hyaline inclusions (alcoholic hyalin, Mallory bodies) are found in livers of patients with a number of hepatic disorders, although they are most common in alcoholic liver disease. Tissues from patients with primary biliary cirrhosis, jejunoileal bypass, hepatocellular carcinoma, Wilson's disease, and Indian childhood cirrhosis were all positive for hyalin by hematoxylin and eosin staining. Immunocytochemical labeling, using guinea‐pig antiserum specific for alcoholic hyalin, was utilized to determine the extent of crossreactivity between hepatocellular hyalin in these various conditions. This antiserum bound to hyalin in fixed paraffin‐embedded sections of all liver tissues studied as detected by indirect immunoperoxidase labeling. Binding to normal human liver, however, was restricted to light staining at the surface of hepatocytes. Preimmune guinea‐pig serum did not bind to either normal liver or to the test tissues. Our results suggest that hyalin found in a diverse group of liver conditions represents an immunologically related structure and that its formation may involve a common me

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1982.tb00835.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACT—The purpose of this study was to estimate and compare the inconvenience of six investigations used in hepatology, as experienced by 113 inpatients and imagined by 80 hepatologists. For patients, the investigations, in order of increased inconvenience rate were: (1) venous puncture, (2) BSP clearance, (3) transvenous liver biopsy, (4) percutaneous liver biopsy, (5) laparoscopy and (6) oesogastroscopy. Hepatologists gave a higher inconvenience rate for all investigations than patients and the ranking of the crude inconvenience rate of each investigation was different for hepatologists and patients. For 84% of patients, oesogastroscopy was over 10 times more inconvenient than a venous puncture, whereas only 15% of hepatologists considered oesogastroscopy over 10 times more inconvenient (p<0.001). Multidimensional analysis indicated that hepatologists underestimated the role of pain in the rejection of re‐investigation for transvenous liver biopsy, percutaneous liver biopsy and oesogastroscopy. These results suggest that hepatologists must improve the psychological and pharmacological premedication of these investigations according to the reaction of their patients, especially before oesogastrosc

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1982.tb00836.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACT—Following oral intake or inhalation, halogenated hydrocarbons are metabolized to hepatotoxic intermediates in the liver to only a small extent, the major part being eliminated via the lungs without biochemical transformation. Following intoxication, increased pulmonary elimination of hydrocarbons can be achieved in patients by treatment with CO2‐induced hyperventilation. To investigate the efficacy of this new therapy under exact experimental conditions, female Wistar rats received 2.5 ml CCl4/kg BW by gastric intubation and were then treated with CO2‐induced hyperventilation. In comparison to untreated animals, hyperventilated rats showed only a few signs of hepatic injury by histological evaluation, whereas massive centrolobular necroses and fatty infiltrations were observed in non‐hyperventilated animals. By biochemical assessment, significant decreases of GOT, GPT and GDH activity were observed in the serum, when hyperventilated rats were compared to untreated animals. Moreover, the LD50for CCl4was almost trebled after hyperventilation compared to the non‐hyperventilated animals. The increased LD50, and the biochemical and histological results therefore substantiate the usefulness of CO2‐induced hyperventilation therapy in the treatment of intoxications by hydrocarbons under standardized experimental

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1982.tb00837.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACT—Patterns of intrahepatic immunoglobulin production were investigated by anin vitrobiosynthetic labelling technique which measured the rate of Ig production in liver biopsy fragments. This technique depends on the incorporation of3H‐leucine into proteins synthesized by cells in the biopsy fragment and subsequently released into the culture medium, and precipitation of Ig with monospecific antisera. Intrahepatic Ig production was expressed as counts of radioactivity precipitated/g of liver tissue/24 h. Mean values were high in various inflammatory diseases of the liver, including alcoholic hepatitis (AH) (17 cases), IgG, 87.8, IgA, 105.6 and IgM, 14.7, chronic active hepatitis (CAH) (19 cases), IgG, 86.0, IgA, 56:1 and IgM, 12.6, and acute viral hepatitis (3 cases), IgG, 116.0, IgA, 61.0 and IgM, 32.0, but low in histologically normal livers (6 cases), IgG, 4.5, IgA, 4.8 and IgM, 4.7, alcoholic fatty liver (11 cases), IgG, 9.4, IgA, 11.4 and IgM, 7.1, and miscellaneous non‐inflammatory conditions (10 cases), IgG, 8.7, IgA, 11.1 and IgM, 5.0. Photomicrographs were used to measure the density of plasma cells, expressed as cells/mm2of liver biopsy tissue: mean counts were for AH 5.1, CAH 16.2 and normal liver 0.0. Intrahepatic Ig productionin vitrodid not correlate with the density of plasma cells in biopsy samples from cases of AH or CAH, nor with serum Ig l

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1982.tb00838.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

ABSTRACT—Six patients with post‐infantile giant cell hepatitis (PIGCH) are reported. The age of the patients ranged from 12 to 74 years. One patient had acute giant cell hepatitis and complete restitution was established by biopsy. In five patients, giant cell formation was seen in chronic aggressive hepatitis with cirrhosis. Cirrhosis was demonstrated 5–11 months after the onset of hepatitis. All of these cases later became inactive, but in only one was evidence of inactivity obtained by liver biopsy. Giant cell hepatitis occurred in 0.25% of patients with post‐infantile hepatitis; however, all but one of the PIGCH‐cases were seen between 1972 and 1974. Hepatitis B markers were negative in all tested cases. The possibility of a special type of infection is

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1982.tb00839.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY