摘要:

Abstract—A numerical scoring system was applied and compared to the conventional histological classification to assess the histological status of liver specimens from 37 patients with chronic posttransfusion non‐A, non‐B hepatitis followed for 7 to 105 months (mean 35 months). Four histological categories of alterations were assessed and scored: piecemeal necrosis (PMN), fibrosis and cirrhosis, lobular necrosis and portal inflammation. Sequential liver biopsies were obtained from 19 patients. PMN was generally mild but still predictive of progressing fibrosis. Thus, in none of the biopsies from four patients with initial PMN score 0 was there any increase in the fibrosis score in the follow‐up biopsy, while in 10/15 (67%) patients with an initial PMN score of ± 1 the fibrosis score increased with time (p = 0.033). Lobular necrosis and portal inflammation were not predictive of progressing fibrosis. Judging from the scoring method, 22% of all the 37 patients displayed cirrhosis and 27% bridging fibrosis in the latest liver biopsy performed. Patients with antibodies to hepatitis C did not differ in histological status or outcome from those without antibodies to hepatitis C. It is concluded that the scoring system can be used to monitor the histological long‐term follow‐up in patients with chronic posttransfusion non‐A, non‐13 hepatitis, and offers a means of predicting the hist

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1990.tb00467.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract—Mallory bodies are known to occur in hepatocellular carcinoma. The simple question whether or not there are any clinicopathological features characterizing Mallory body‐positive hepatocellular carcinoma remains unresolved to date. The present study of 200 consecutive autopsy cases of hepatocellular carcinoma showed several important differences between 49 cases bearing Mallory bodies and 151 cases bearing no Mallory bodies in carcinoma cells. The patients in the former group were older, showed a higher association rate of liver cirrhosis, and their liver weight was lighter. As to the gross pathology of hepatocellular carcinoma, the nodular type was relatively frequent in Mallory body‐positive hepatocellular carcinoma, while the massive and diffuse types were relatively frequent in Mallory body‐negative cases. The frequency of extrahepatic metastases in the Mallory body‐positive group was lower than that in the Mallory body‐negative cases. The reasons for these differences remain

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1990.tb00468.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract—A silver colloid technique to identify argyrophilic nucleolar organizer region associated protein (AgNOR) was applied to 43 cases of intrahepatic bile duct carcinoma (cholangiocarcinoma, CC), 2 with bile duct adenoma (BDA), 5 with focal duct epithelial hyperplasia (FEH) associated with hepatolithiasis, 15 with posthepatitic ductular proliferation (PHDP) associated with massive or submassive hepatic necrosis and 20 of normal liver. In the present study, only discrete, easily counted black dots within nuclei and silver‐stained nucleolus were counted under a magnification of X 400 without oil‐immersion objectives. The mean AgNOR count of CC was significantly higher than those of BDA, FEH, PHDP and normal controls (P>0.05, P>0.001, P>0.01, and P>0.001, respectively). Among CCs the mean AgNOR numbers of papillary adenocarcinoma (pap), moderately (tub2) and poorly differentiated (por) adenocarcinoma, and adeno‐squamous carcinoma (as) were significantly higher than that of normal controls (P>0.01, P>0.001, P>0.001 and P>0.001, respectively), and those of tub2, por and as were also significantly higher than those of BDA, FEH and PHDP, whereas that of well differentiated tubular adenocarcinoma (tub 1) was not different from those of BDA, FEH, PHDP and normal controls, and that of pap was not different from those of BDA, FEH and PHDP. The mean numbers of AgNORs of BDA and FEH were not different from that of normal controls, whereas that of PHDP was significantly higher than that of normal controls (P>0.01). Interestingly, the mean AgNOR counts of tubular adenocarcinoma were increased with histologic tumor grades. All cases with AgNOR counts of more than 2.24 had CC. Furthermore, not only quantitative but also striking qualitative abnormalities of AgNORs were seen in CCs. Large and/or irregularly shaped AgNORs without uniformity in size and density were characteristic for CCs, even in well differentiated adenocarcinoma, in contrast to the small, round, regular AgNORs seen in normal bile duct epithelium and rather large but regular AgNORs seen in benign bile duct lesions. These results indicate that the enumeration of AgNORs in various bile duct lesions is useful to identify moderate and high grade CCs and to evaluate tumor grade, but not useful to discriminate well differentiated adenocarcinoma from benign lesions. In case of normal or equivocal AgNOR counts, the presence of large and/or irregularly shaped AgNORs without uniformity in size and density would favor a diagnosis of carcinoma rather than benign count

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1990.tb00469.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract—The present study is concerned with changes in the number and localization of S‐phase cells in the liver of rats exposed to dimethylnitrosamine (DMN). S‐phase cells were detected by immunohistochemistry after injection of bromodeoxyuridine (BrdU) and exposure of paraffin sections of liver tissue to the antibody anti‐BrdU. With respect to controls, the number of S‐phase cells increased four to fivefold in DMN‐treated animals in the first week of treatment and remained significantly higher thereafter, in association with the formation of septa. At all times, the labelling index was higher in littoral cells than in hepatocytes. No labelling was observed in biliary cells. This behaviour is different from that reported in other situations, for instance in regeneration after partial hepatectomy, which suggests that besides hepatocytes and littoral cells replacement, an involvement of the latter cell line in the inflammatory reaction, synthesis of extracellular matrix components and formation of septa may account for this particu

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1990.tb00470.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract—Transforming growth factor beta (TGFB) is a potent inhibitor of DNA synthesis in adult rat hepatocytesin vitro.In the present study, the response of hepatocytes from normal or regenerating rat liver to TGFB was determined. TGFB inhibited DNA synthesis uniformly in hepatocytes from both groups in the absence of EGF. However, hepatocytes from 3 h regenerating liver maintained for 3 days in the presence of EGF were less sensitive to the growth‐inhibitory action of TGFB. [3H]‐thymidine incorporation was inhibited at 20 pM TGFB by only 7% in hepatocytes from 3 h regenerating liver compared with 70% in normal hepatocytes. By increasing the dose of TGFB to 100 pM, however, the full inhibitory response was restored. Reduced sensitivity was also found when the nuclear labelling index was determined, but no change was observed in cells from rats 3 h following sham hepatectomy. The change in sensitivity to TGFB required the presence of 5 ng/ml EGF or greater. Within a further 24–48 h in culture, the response to lower doses of TGFB was at least partially restored. While the present experimental design cannot directly confirm the role of TGFB as a paracrine inhibitor of liver growthin vivo, the data are compatible with this hyp

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1990.tb00471.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract—Hepatitis delta virus (HDV) superinfection of woodchuck chronic carriers of woodchuck hepatitis virus (WHV) results in acute and chronic disease. The different courses of disease mimicked the outcome of human HDV superinfection, making woodchucks valuable models for clinical studies of HDV. Ten of 11 woodchuck chronic carriers of WHV superinfected with HDV developed acute HDV infection with markers of viral replication in the serum and liver. One animal (DW128) had no serological markers of acute HDV infection. Nine of 11 (82%) superinfected animals developed chronic HDV infection. An unusual course of chronic HDV infection occurred in one woodchuck (DW128): no serum markers of acute or chronic HDV infection appeared but HDV RNA was detected in the liver, indicating that chronic HDV infection can occur without serological marker

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1990.tb00472.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract—We investigated the inhibitory effects of purified recombinant hepatitis B virus (HBV) surface antigen (rHBsAg) and core antigen (rHBcAg) on lymphokine‐activated killer cell (LAK) activity. Either peripheral blood mononuclear cells (PBMCs) or CD16+CD3‐LAK precursors, both of which were pre‐incubated with interleukin‐2 (IL‐2) and rHBsAg or rHBcAg for 72 h, showed a significant decrease in LAK cytotoxicity against Daudi cells, in comparison to the results recorded in the presence of IL‐2 alone, or IL‐2 andE. coliextracts. This inhibitory effect was dose‐dependent and was observed to be time‐dependent from 24 to 72‐h‐cultures with these HBV antigens. This influence was not mediated with either adherent cells or other accessory cells. The proliferative reaction of either PBMCs or the LAK precursors after being cultured with IL‐2 and rHBsAg or rHBcAg for 72 h was significantly diminished compared with the levels of reaction of those cells after a 72‐h culture with IL‐2 alone or with IL‐2 andE. coliextracts. The levels of IL‐2‐driven IL‐2 receptor (p55) expression of either PBMCs or the LAK precursors in the presence of rHBsAg or rHBcAg were higher than the levels seen in the absence of these HBV antigens. These results suggest that HBsAg and HBcAg may inhibit the induction of LAK activity by interfering with the proliferative reaction of the LAK precursors to IL‐2 without inhibiting the IL‐2 receptor expression of the cells. Cytofluorographic analysis of PBMCs, cultured with rIL‐2, showed lower percentages of CD3+ and CD16+ cells in the presence of these HBV ant

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1990.tb00473.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract—It has been documented that chronic hepatitis may progress to cirrhosis and then develop hepatocellular carcinoma (HCC). To test whether abnormal cellular DNA increases along this line of development, liver tissues from 48 patients with chronic hepatitis, 17 with cirrhosis, and 8 with HCC were investigated for cellular DNA content with a scanning microdensitometer. Seven of 8 HCCs and 2 cirrhotic livers adjacent to HCC had abnormally increased cellular DNA content. Only 4 livers from patients with chronic liver diseases other than HCC had abnormal cellular DNA content. The cellular DNA content in livers not accompanying HCC was not related to the patient's age, histological diagnosis, and hepatitis inflammatory activity. The results confirmed the increase of cellular DNA content in HCC, but did not provide evidence of a progressively increasing DNA content from chronic hepatitis to liver cirrhosis. However, cirrhotic livers with abnormal hepatocytic DNA content deserve careful follow‐up for the early detection of

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1990.tb00474.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1990.tb00475.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY