摘要:

ABSTRACT—Glafenine was associated with hepatic injury in 38 cases. The causal relationship was assessed on the basis of the temporal relationship with drug use, course and exclusion of other causes. In 27 cases a causal relationship was considered likely, i.e. ‘probable’ (12 cases) or ‘possible’ (15 cases), whereas in 11 cases it was either unlikely or unclassifiable. In both the ‘probable’ and ‘possible’ groups 60–70% of individuals were women. Jaundice was present in three‐quarters of cases in both groups. Eosinophilia was more frequent in the group of ‘probable’ cases, and this group had the highest case‐fatality rate (42%). Onset varied from 2 days (after a rechallenge) to 8 months, but most cases appeared between 2 weeks and 4 months after starting therapy. Histology in 22 cases showed a predominantly hepatocellular pattern, varying from spotty panlobular necrosis, centrilobular and (sub)massive necrosis (acute pattern) to fibrosis and cirrhosis (chronic pattern). The chemical structure of glafenine and the clinicopathological pattern it induces resemble that of cinchophen. The incidence is unknown. Either metabolic idiosyncrasy or an immunoallergic mechan

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1986.tb00270.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

ABSTRACT—Serum samples from 608 residents of Saudi Arabia were examined for the presence of hepatitis B, delta and HTLV‐III virus markers. The HBsAg carrier rate was 17% and the prevalence of all hepatitis B virus markers was 55%. Males between 16 and 25 years had a significantly higher prevalence of all HBV markers (69%; 95% confidence limits: 49%–82%) compared to females in the same age group (34%; 95% confidence limits: 25%–45%). The prevalence of HBsAg was significantly higher among males aged 25–35 years (24%; 95% confidence limits: 19%–40%) compared to females in the same age group (9%; 95% confidence limits: 4%–18%). In addition, the HBsAg carrier rate for all males (23%) was significantly higher compared to females (11%) (p<0.001). HBeAg was found in 12% of 89 HBsAg‐positive individuals, 48% were anti‐HBe positive and 40% were negative for both markers. Hepatitis B virus DNA was found in 11 of 41 individuals tested. Five of these were HBeAg positive and four were anti‐HBe positive. Anti‐delta was found in three of 36 HBsAg positive serum samples (8%). Of 485 individuals tested for anti‐HTLV

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1986.tb00271.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

ABSTRACT—The distribution of microfilaments in cryostat sections of liver from BALB/c and C57BL/6 mice was compared using the F‐actin binding probe rhodaminyl phalloidin and indirect immunofluorescence using a human serum containing anti‐actin autoantibodies. The immunological reactivity of this serum was established by its capacity to immunoprecipitate purified skeletal muscle actin and by its ability to immunoprecipitate a protein which migrated electrophoretically with actin from35S‐labeled liver cells. Oral administration of the liver toxin sporidesmin did not substantially alter the binding of rhodaminyl phalloidin to microfilaments but the reactivity of the anti‐actin serum with the liver cytoskeleton was diminished 3 h after, and enhanced within 24 h of toxin ingestion. Amounts of actin measured by DNAase inhibition were not altered. The results are assessed in terms of their significance for understanding the way in which sporidesmin causes liv

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1986.tb00272.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

ABSTRACT—More than 25% of undirected percutaneous liver needle biopsies that are performed in search of metastatic neoplasms yield false negative specimens. Often, however, histologic changes are observed in the liver adjacent to space‐occupying lesions due to focal obstruction of bile and blood flow. These alterations were defined by semi‐quantitative evaluation of 20 liver biopsy specimens with metastatic neoplasms, and revealed a characteristic histologic triad consisting of proliferated and abnormal bile ductules, polymorphonuclear leukocytes in edematous portal tracts and focal sinusoidal dilatation. When 15 patients with this combination of histologic alterations, but without malignant cells in liver biopsy specimens, were investigated prospectively, 13 patients (87%) were found to have hepatic metastases. Therefore, the observation of the histologic triad described here should lead to a continued search for space‐occupying lesions in th

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1986.tb00273.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

ABSTRACT—Perfusion fixation is essential for the examination of the hepatic microvasculature by scanning electron microscopy. No detailed study has previously been done on the perfusion of human hepatic needle biopsies. The present paper demonstrates two modifications of a simple technique by which needle biopsies can be perfused. Perfusion was performed either manually (n = 33) or with a constant perfusion pressure of 10–15 mm H2O (n = 54). No significant difference in the ultrastructural preservation was observed in the two groups. Manual perfusion, however, when done gently, was superior in cleaning the sinusoids, and thus larger areas could be investigated by this method. Topographical orientation was possible, allowing the identification of terminal hepatic veins and portal tra

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1986.tb00274.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

ABSTRACT—The scanning electron microscope was used to study the fenestrations of human liver sinusoids. Thirteen biopsies, where light microscopy and transmission electron microscopy revealed normal sinusoidal architecture, were investigated. The number of fenestrae was calculated in acinar zone 3 and acinar zone 1 areas. The porosity, i.e. fenestrated area relative to total area of the sinusoidal lining wall, was estimated on micrographs by using a Texture Analysing System (TAS). A zonal gradient was found with median 23.5 fenestrae per μm2in zone 3, as compared to 19.2 fenestrae per μm2in the zone 1 area (p<0.005). Increasing porosity towards the terminal hepatic vein was found (9.1% in zone 3 vs. 7.6% in zone 1 (p<0.05)). This was due to an increased number of fenestrae measuring 100 nm or less in diameter in zone 3 areas. The number of fenestrae measuring more than 100 nm but less than 300 nm was unchanged along the sinusoids. Fenestrae larger than 300 nm were rare and randomly distributed. The results suggest a different permeability along the sinusoidal lining, which may influence the transport between blood and hepatocytes and thus be of importance to hepatocellular funct

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1986.tb00275.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

ABSTRACT—Trace elements (Fe, Cu, Zn, Se, Mn, Ti, Pb) were measured by X‐ray fluorescence spectrometry in normal liver tissue obtained at autopsy from 74 subjects (44 ♀, 30 ♂), median age 62 years (range 20–87), and in tissue from 27 cirrhotic livers (14 alcoholic, 13 non‐alcoholic cirrhosis). The element content (median and 5–95 percentile interval) innormal liversin mmol/kg dry tissue was:Fe, 16.51 (7.82–39.03);Cu, 0.378 (0.189–0.629);Zn, 4.01 (2.59–9.33);Se, 0.018 (<0.004–0.035);Mn,<0.055 (<0.055–0.237);Ti,<0.146 (<0.146–0.919);Pb,<0.0005 (<0.0005–0.0154). Only copper content showed a sex difference, being higher in males than in females (P<0.04). In both groups ofcirrhotic liver, Fecontent was within normal,Cucontent above normal (P<0.05, P<0.02), andSecontent below normal (P<0.0001, P<0.04). Alcoholic cirrhotic livers had lowerZnlevels (P<0.02), higherMnlevels (P<0.06), and higherPblevels

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1986.tb00276.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

ABSTRACT—Eight patients with chronic (mean 52 months) type B hepatitis (chronic active hepatitis and active cirrhosis) and markers of active viral replication (presence of HBeAg and HBV‐DNA in serum) were treated with Ara‐AMP for 7–12 weeks. The mean follow‐up time was 18.3 months. All but one patient responded to treatment. In seven patients, HBeAg and HBV‐DNA became negative and developed anti‐HBe. One patient lost HBsAg as well as HBeAg and HBV‐DNA. HBcAg in liver tissue became undetectable or greatly reduced in the seven patients in whom it was strongly positive. Aminotransferase and immunoglobulin levels reverted to normal in the seven responders and remained normal through the entire follow‐up period. A second liver biopsy, performed after completion of therapy, showed improvement in six of eight cases, with disappearance of lobular activity. Two patients treated for 12 weeks developed a severe polyneuropathy lasting for 6 months. A 7‐week course of Ara‐AMP seems to induce long‐lasting inhibition of viral replication, along with an improvement in liver function tests and liver histology, without significant side effects. On the other hand, a 12‐week course was associated with the development of a severe and

ISSN:0106-9543    

DOI:10.1111/j.1600-0676.1986.tb00277.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY