摘要:

AbstractNewborn mouse spleen, whose cells strongly suppress thein vitrohumoral response of adult spleen cells, is essentially a hematopoietic organ. It contains a large percentage of proliferating cells, among which about 50% are erythroblasts (identified by their spectrin content) and about 15% are cells of the myelocytic and monocytic lineage. Lymphoid cells are a minority, with about 20% B and only 1‐2% T lymphocytes. After 4 days, a culture of newborn spleen cells contains 5‐10 times more macrophages than that of an adult spleen. Most of these macrophage precursors from the newborn spleen are proliferating cells, partially glass‐ or plastic‐adherent, which differentiate in culture into activated macrophages producing large amounts of plas‐minogen activator. It is this macrophage excess which is responsible for the immunosuppressive effect of newborn spleen cells in culture, as indicated by (a) the effect of silica particles added to the cultures, which both relieve the suppression and prevent the accumulation of macrophages and (b) the suppression of the humoral response of adult spleen cells when they are cultured on the adherent cells from a newborn but not from an adult spleen.The suppressive effect of macrophages seems to result, at least in part, from the production of prostaglandin, since it can be relieved by indomethacin or aspirin. Suppression is not related to arginine depletion of the medium or to production of an excess of plasminogen activator. T lymphocytes from newborn spleen or lymph nodes have no suppressive c

ISSN:0014-2980    

DOI:10.1002/eji.1830110112

出版商:WILEY‐VCH Verlag GmbH    

年代:1981

数据来源: WILEY

摘要:

AbstractTwo allospecific T cell lines, C.C3.11.75 (H‐2danti‐H‐2k) and B6.C.7.76 (H‐2banti‐H‐2d), established from mixed lymphocyte cultures, were selected by continuous antigenic stimulation over several years. Both cell lines proliferated to alloantigen, provided allohelp to B cells in the humoral antibody response, and one of them showed cytolytic activity. Using spleen cells from various congenic mouse strains, the specificity of antigen recognition was examined in order to examine whether there is a correlation between T cell function and antigen recognition. Results showed that in all functional assays, the same antigenic determinant, a private specificity encoded in IA, was recognized. This shows that there is no obligatory correlation between T cell function and antigen recognition and may suggest that the T cell receptor repertoire is biased towards recognizing private sp

ISSN:0014-2980    

DOI:10.1002/eji.1830110113

出版商:WILEY‐VCH Verlag GmbH    

年代:1981

数据来源: WILEY

摘要:

AbstractCells of the hybridoma cell line X 63/38 C‐13 secreting both IgM and IgG have been used in studies on the importance of the carbohydrate moieties of the Ig molecules for their secretion. It has been found that the secretion of the IgM produced in the presence of the glycosylation inhibitor tunicamycin was strongly inhibited, while the secretion of the IgG produced by the same cells was only slightly affecte

ISSN:0014-2980    

DOI:10.1002/eji.1830110114

出版商:WILEY‐VCH Verlag GmbH    

年代:1981

数据来源: WILEY

摘要:

AbstractIn the syngeneic mixed lymphocyte reaction, Ly 1 cells proliferate following stimulation with Ia‐positive macrophage‐like cells. The proliferation is accompanied by the release of interleukin 2 into the medium. Both the proliferation and interleukin 2 production are dependent on the expression of Ia antigenic determinants, as the addition of haplotype‐specific anti‐Ia antisera to the cultures inhibits both par

ISSN:0014-2980    

DOI:10.1002/eji.1830110115

出版商:WILEY‐VCH Verlag GmbH    

年代:1981

数据来源: WILEY

ISSN:0014-2980    

DOI:10.1002/eji.1830110101

出版商:WILEY‐VCH Verlag GmbH    

年代:1981

数据来源: WILEY