摘要:

AbstractThe relationship of serologically defined determinants to determinants recognized by cytotoxic T cells on molecules encoded by the Kkgene of the murine major histocompatibility complex (H‐2) has been analyzed. For this purpose we used three somatic variants of a Kk‐expressing lymphoma line lacking individual determinants of the Kkmolecule, as defined by monoclonal antibodies (mAb), as target cells for Kk‐specific alloreactive and Kk‐restricted cytotoxic T lymphocytes (CTL) cloned by limiting dilution. Neither alloreactive nor fluorescein isothiocyanate, influenza‐ or Newcastle disease virus‐specific Kk‐restricted CTL clones were found to distinguish between variants and wild type cells, indicating that the serologically defined determinants lost by the variants were not essential for antigen recognition of CTL with these specificities. On the other hand, two of the variants lacking either one of a pair of serological determinants were discriminated from Kkwild type cells by about 40% of Kk‐restricted, trinitrophenol (TNP)‐specific CTL clones. The third variant, lacking both of the determinants, however, was lysed by all CTL clones to the same extent as wild type cells. From these results we conclude that the determinants restricting the TNP‐specific CTL were also not identical with those defined by mAb.In experiments performed to optimize the conditions for the limiting dilution analysis we found that the specificity of the CTL stimulation was strongly dependent on the concentration of T cell growth factor (interleukin 2) in the cultures during CTL stimulation. High concentrations of IL2 resulted in a drastic increase in the frequency of CTL clones. Part of these clones, however, were found not to be specific for antigens present on t

ISSN:0014-2980    

DOI:10.1002/eji.1830131012

出版商:WILEY‐VCH Verlag GmbH    

年代:1983

数据来源: WILEY

摘要:

AbstractA requirement for idiotype matching was reported such that carrier‐primed T helper cells from mice, which are unable to produce an idiotype (idiotype‐negative) because of genetic reasons or adult anti‐idiotype or neonatal anti‐μ antibody treatment, did not provide helper function for an idiotype‐positive antibody response.We have analyzed the requirement for idiotype matching in the response to phosphorylcholine by using mice which are idiotype‐negative because they were injected with anti‐idiotype antibody shortly after birth. Carrier‐primed T cells from such animals supported idiotype‐positive responses when mixed with normal or primed B cell populations and challenged with appropriate antigen; these responses were quantitatively and qualitatively similar to those obtained with T cells from idiotype‐positive animals. These results demonstrate no requirement for idiotype matching and suggest that the procedure used to establish an idiotype‐negative T cell donor may be decisive in showing a requirement

ISSN:0014-2980    

DOI:10.1002/eji.1830131013

出版商:WILEY‐VCH Verlag GmbH    

年代:1983

数据来源: WILEY

摘要:

AbstractThe mouse monoclonal antibodies W3/25 and MRC OX‐8 have been used to distinguish rat T lymphocyte subpopulations. In the peripheral T lymphocyte population, W3/25 antibody recognizes an antigen on the Thelper(Th) subset, while MRC OX‐8 antibody recognizes an antigen on the Tsuppressor/cytotoxic(Ts/c) subset. To determine the nature of these antigens, rat thymocytes were either metabolically labeled with [35S]L‐methionine or surface‐labeled at sialic acid residues by periodate oxidation followed by [3H]NaBH4reduction. Thymocytes were solubilized with nonionic detergent, the antigens immunoprecipitated and the molecular weights determined by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis analysis. Using metabolically labeled cells, W3/25 antibody immunoprecipitated an antigen that electrophoresed under reducing conditions as a broad band between 48000–53000 Mr. Unreduced samples migrated between 46000–50000 Mr. Surface‐labeled W3/25 antigen, electrophoresed under reducing conditions, separated into two bands of 44000 and 52000 Mr. Metabolically labeled MRC OX‐8 antigen was identified as a protein of at least two chains of 39000 and 34000 Mr. There was also a fainter band at approximately 67000 Mr. Unreduced samples indicated a more complex structure with bands at 70000, 110000 and 165000 Mr. Surface‐labeled MRC OX‐8 antigen was of similar nature. These data, when considered with functional and tissue distribution data, suggest that W3/25 antigen is equivalent to T4 in man and MRC OX‐8 antigen is equivalent to T8 in

ISSN:0014-2980    

DOI:10.1002/eji.1830131014

出版商:WILEY‐VCH Verlag GmbH    

年代:1983

数据来源: WILEY

摘要:

AbstractThe influence of muramyl dipeptide (N‐acetylmuramyl‐L‐alanyl‐D‐isoglutamine) on the rejection of mouse skin allografts was investigated. While the 0.1‐mg dose administered on days 7, 6, 5 prior to transplantation caused significant prolongation of the graft survival, the 0.5‐mg dose administered on days 3,2,1 prior to transplantation resulted in remarkable augmentation of the graft rejection. The present results support the view that muramyl dipeptide can induce both stimulatory and suppressive immune mechanisms, depending on the trea

ISSN:0014-2980    

DOI:10.1002/eji.1830131015

出版商:WILEY‐VCH Verlag GmbH    

年代:1983

数据来源: WILEY

ISSN:0014-2980    

DOI:10.1002/eji.1830131016

出版商:WILEY‐VCH Verlag GmbH    

年代:1983

数据来源: WILEY

ISSN:0014-2980    

DOI:10.1002/eji.1830131001

出版商:WILEY‐VCH Verlag GmbH    

年代:1983

数据来源: WILEY