摘要:

AbstractThe binding of antigen to the multicomponent T cell antigen receptor (TcR)‐CD3 complex leads to the activation of several signal transduction pathways which result in T lymphocyte proliferation and lymphokine secretion by molecular mechanisms and catalytic molecules as yet poorly defined. One of the earliest events that follows the triggering of the antigen‐specific TcR‐CD3 complex is a rapid tyrosine phosphorylation of several intracellular substrates, suggesting stimulation of at least one protein tyrosine kinase (PTK). Since none of the seven TcR‐CD3 subunits exhibits a recognizable kinase domain, it seems likely that the receptor complex is associated with an intracellular PTK. p59fynand the T lymphocyte‐specific p56lckare two intracellular, non‐receptor, cell membrane‐associated PTK of thesrcfamily expressed in T lymphocytes. Here, we show by double immunofluorescence microscopy a specific co‐distribution of p59fyn, but not p56lck, with antibody‐induced TcR or CD3 caps in intact human T lymphocytes. These findings provide direct evidence for a significant association of p59fynwith the TcR‐CD3 complex under physiologically relevant conditions in functional T lymphocytes. They suggest that p59fynis a crucial component of the TcR signal transduction machinery and that one of the earliest consequences of antigen recognition by the TcR is p59fyn‐mediated phosphorylation of intracellular substrates

ISSN:0014-2980    

DOI:10.1002/eji.1830220142

出版商:WILEY‐VCH Verlag GmbH    

年代:1992

数据来源: WILEY

摘要:

AbstractMost soluble protein antigens are poor at priming class I‐restricted cytotoxic cell activity. In this study we show that incorporation of one protein, ovalbumin, into liposomes converts this antigen into an effective stimulator of antigen‐specific cytotoxicity. The mechanisms responsible for this phenomenon are discus

ISSN:0014-2980    

DOI:10.1002/eji.1830220143

出版商:WILEY‐VCH Verlag GmbH    

年代:1992

数据来源: WILEY

摘要:

AbstractIn this report we have compared responses of T cells bearing different T cell receptor (TcR) Vβdomains, including Vβ6, 7, 8.1 and 9, to the minor lymphocyte stimulatory locus (MIs‐1a). The virtually complete deletion of peripheral T cells using these TcR Vβdomains in mice expressing MIs‐1averified their MIs‐1areactivity. However, varied responses among these cells following stimulation with MIs‐1ain vitrosuggested a hierarchy of TcR reactivities for MIs‐1awith Vβ6 and Vβ9>Vβ8.1 » Vβ7. This hierarchy was further supported byin vivostudies which showed that Vβ6+CD4+cells dominated r

ISSN:0014-2980    

DOI:10.1002/eji.1830220144

出版商:WILEY‐VCH Verlag GmbH    

年代:1992

数据来源: WILEY

ISSN:0014-2980    

DOI:10.1002/eji.1830220145

出版商:WILEY‐VCH Verlag GmbH    

年代:1992

数据来源: WILEY

ISSN:0014-2980    

DOI:10.1002/eji.1830220101

出版商:WILEY‐VCH Verlag GmbH    

年代:1992

数据来源: WILEY