ISSN:0014-2980    

DOI:10.1002/eji.1830091202

出版商:WILEY‐VCH Verlag GmbH    

年代:1979

数据来源: WILEY

摘要:

AbstractThe heterogeneity of the anti‐GAT [terpolymer poly(Glu60, Ala30, Tyr10)] response of GAT responder mice has been analyzed. Purified anti‐GAT antibodies from BALB/c mice belong only to the γ1χ subclass. The isoelectric focusing pattern obtained indicates that the anti‐GAT antibodies are particularly basic and restricted. These results have been confirmed by two‐dimensional polyacrylamide gel electrophoresis; by this technique, we have shown that the γ1chain and the corresponding χchain of anti‐ GAT antibodies are restricted. All the anti‐GAT antibodies from 10 BALB/c mice bear the cross‐reactive GAT idiotype previously defined (J. Thèze and G. Sommé,Eur. J. Immunol.1979,9: 294). The present results indicate that the anti‐GAT repertoire expressed in BALB/c

ISSN:0014-2980    

DOI:10.1002/eji.1830091203

出版商:WILEY‐VCH Verlag GmbH    

年代:1979

数据来源: WILEY

摘要:

AbstractMouse mastocytoma cells (P815) formed rosettes with normal mouse spleen lymphocytes which had been coated with uncleaved human C 3; this interaction was clearly dependent on the amount of C 3. Lymphocytes treated with C 3 b or buffer alone were ineffective. Formation of cell contact could be inhibited by the presence of protease inhibitors such as diisopropyl fluorophosphate, phenyl methyl sulfonyl fluoride and tosyllysyl chloromethyl ketone. Seven out of 13 different cell lines behaved like P 815 cells.The results strongly suggested that a proteolytic activity on mouse tumor cells led to a cooperation with uncleaved C3 on a carrier cell to connect these two cells. We interpreted these data in analogy to the complement‐dependent bridge formation mechanism (M. P. Dierich and B. Landen,J. Exp. Med.1977.146: 1484): uncleaved C3, attached to mouse spleen lymphocytes as carriers, becomes cleaved by enzymes associated with the tumor cells tested; by this cleavage, the labile binding site is released on C3 (nascent C3b) and anchors the C3‐carrying cell to the protease‐ carrying cell; since this labile binding site is short‐lived, this process can be induced by membrane‐associated proteases only. The nature of the proteases and the biological implications of this process are as yet

ISSN:0014-2980    

DOI:10.1002/eji.1830091204

出版商:WILEY‐VCH Verlag GmbH    

年代:1979

数据来源: WILEY

摘要:

AbstractThe immunoglobulin (Ig) classes and subclasses of the specific antibodies contained in antisera raised in male 129/Sv mice against cells of a syngeneic clonal line of embryonal carcinoma (F9) have been determined. Cytotoxic activity was found associated almost exclusively with anti‐F9 IgM (μχ) antibodies. A large part of anti‐F9 activity was found associated with IgG1(γ1χ and presumably also γ1λ) antibodies, and was detectable only by indirect immunofluorescence. Traces of specific IgG2aand IgG2bantibodies were also found. No IgG3and IgA antibodies reacting with embryonal carcinoma cells were detectable under these conditions. The serum of F9 tumor‐bearing animals had a very similar Ig composition. fürthermore, IgM and IgG1, but not IgG2antibodies, were detected at the surface ofin vivogrowing F9

ISSN:0014-2980    

DOI:10.1002/eji.1830091205

出版商:WILEY‐VCH Verlag GmbH    

年代:1979

数据来源: WILEY

摘要:

AbstractRabbit antiserum against mouse brain tissue (anti‐brain‐associated T cell antigen, anti‐BAT) was capable of killing splenic natural killer (NK) cells of CBA/J, BALB/c, C 57 BL/6 J, C 3 H/He and nude mice, which were detected with Molony virus‐induced lymphoma (YAC‐1) and radiation‐induced leukemia (RL ♂ 1) cells as targets. The same antiserum abolished T cell functions,e.g.carrier‐specific helper function and the responsiveness to concanavalin A, but not B cell functions,e.g.immunological memory for the secondary antibody response and the responsiveness to lipopolysac‐ charide. After absorption of the anti‐BAT with thymocytes, the ability to kill T cells was completely abrogated, leaving the activity to kill NK cells intact. No other heterologous and isologous antisera,i.e.rabbit anti‐mouse thymocyte antiserum, goat antiserum against antigens shared by thymus and B cells, anti‐Thy‐1.2 and anti‐la antisera, could eliminate NK function regardless of their definite reactivity against T or B cells. The results indicate that the absorbed anti‐BAT can distinguish NK cells from other k

ISSN:0014-2980    

DOI:10.1002/eji.1830091206

出版商:WILEY‐VCH Verlag GmbH    

年代:1979

数据来源: WILEY

摘要:

AbstractActivation of human T cells by mitogens was compared in cultures containing serum, human serum albumin or purified human transferrin as growth support. The mitogenic effect of the lectins leucoagglutinin, concanavalin A andWistaria floribundaagglutinin was measured as incorporation of [3H]thymidine into the cellular DNA of the lymphocytes. Three different preparations of transferrin were all able to fully substitute serum or serum albumin as growth promotors, when present at concentrations of 10 μg/ml or more. A small contamination of transferrin in the human serum albumin preparations used was shown to be responsible for their growth‐supporting effect, while no need for the presence of albumin itself could be demonstrat

ISSN:0014-2980    

DOI:10.1002/eji.1830091207

出版商:WILEY‐VCH Verlag GmbH    

年代:1979

数据来源: WILEY

摘要:

AbstractAn antiserum raised in BALB/c AnN mice against selected CAL.20 T cells reacts with a cell surface antigen in virgin animals that is found on 25% of mature thymocytes and Lyt‐2‐bearing T cells, but not on prothymocytes, Lyt‐1 T cells or B cells. The antigen is restricted to strains carrying the Ig‐ldor Ig‐leheavy chain allotype haplotypes. It is expressed in F1mice. The antiserum blocks the binding of suppressor T cells to the cross‐reacting idiotype for arsonate while reagents specific for Fab, Fc or Ig were ineffective. We suggest that the antigen described represents a determinant on the product of a new locus coding for a heavy chain‐linked polypeptide found on a subpopulati

ISSN:0014-2980    

DOI:10.1002/eji.1830091208

出版商:WILEY‐VCH Verlag GmbH    

年代:1979

数据来源: WILEY

摘要:

AbstractThe partial N‐terminal amino acid sequences and tryptic peptide maps of I‐A subregion products from the H‐2b, H‐2d, H‐2kand H‐2Shaplotypes demonstrate that haplotype‐associated differences are present in both a and β polypeptides. The genetic and evolutionary implications of these multiple amino acid substitutions and the homology relationships among Ia molecules from human, guinea pig and the I‐E subregion of the mou

ISSN:0014-2980    

DOI:10.1002/eji.1830091209

出版商:WILEY‐VCH Verlag GmbH    

年代:1979

数据来源: WILEY

摘要:

AbstractMurine and human β2‐microglobulin (β2m) bind to various types of mouse cells. The binding is saturable and displays a single association constant of about 1 × 109liter/mol. The binding of β2m to splenocytes was not affected by a variety of metabolic inhibitors but was temperature‐dependent. It is suggested that the β2m “receptor” exhibits a temperature‐dependent conformational change since the “receptor”, whether integrated into the membrane or solubilized by the detergent Triton X‐100, binds β2m poorly at low temperatures. Spleen T and B lymphocytes display more binding sites than thymocytes, kidney, liver and brain cells. The relative amounts of the β2m‐binding “receptor” on these cell types are strongly correlated to the relative amounts of H‐2 antigens. This correlation is also obvious for the teratocarcinoma cell line F9, which lacks both β2m “receptor” and H‐2 antigens, but spermatozoa, which express very small amounts of H‐2 antigens, have an appreciable amount of the β2m “receptor”. The latter observation, together with the fact that alloantisera directed against H‐2 K and D antigens do not measurably affect the binding of β2m to the “receptor”, may argue against the notion that the β2m “receptor” represents H‐2 antigens which have lost their endogeneous β2m. Normal mouse serum contains a component which inhibits the binding of β2m to splenocytes. It is likely that this serum protein is identical to a newly discovered H‐2 antigen‐like glycoprotein. The β2m “receptor” appears to be under the control of the major histocompatibility complex as splenocytes of the H‐2fha

ISSN:0014-2980    

DOI:10.1002/eji.1830091210

出版商:WILEY‐VCH Verlag GmbH    

年代:1979

数据来源: WILEY

摘要:

AbstractA unique subpopulation of macrophages (MΦ) was identified among the spleen and bone marrow MΦ of normal mice. After 24 h of culture, approximately 2.5% of the adherent cells cluster into “foci” of 10–30 cells. On the basis of their phagocytic and morphologic characteristics, these focus‐forming MΦ (FF‐MΦ) appeared to be highly activated. Uncoated sheep erythrocytes (E) were ingested by FF‐MΦ indicating that opsonization was not a prerequisite for phagocytosis. However, IgM‐coated E (EIgM) were more readily phagocytosed by FF‐MΦ than were E suggesting that IgM is recognized as an effective opsonin by these cells. EIgM and E coated with IgM and complement(C) (ElgMC) were ingested by approximately the same percentage of FF‐ MΦ; thus, if these cells possess complement receptors in addition to structures which bind EIgM, the C receptors do not enhance the ability of FF‐MΦ to ingest opsonized particles.The non‐focus‐forming MΦ,e.g.individual MΦ (I‐MΦ), in the spleen and bone marrow can, themselves, be divided into various subpopulations distinguished by their ability to bind and ingest E, EIgM and ElgMC. These may represent various subpopulations of MΦ or MΦ at various stages of activation or differentiation.While spleen and bone marrow MΦ contained FF‐MΦ and I‐MΦ which vary in their ability to ingest E, EIgM and ElgMC, the MΦ of the peritoneum and blood of normal mice were far more homogeneous. Peritoneal and blood MΦ did not form foci, and did not ingest E or EIgM in significant amounts although a small percentage were able to ingest ElgMC. These data suggest that the population of MΦ in the spleen and bone marrow are far more heterogeneous than those found in the peritoneum or blood and that binding and phagocytosis of various coated and uncoated erythrocyte

ISSN:0014-2980    

DOI:10.1002/eji.1830091211

出版商:WILEY‐VCH Verlag GmbH    

年代:1979

数据来源: WILEY