摘要:

AbstractFour lines of mice were produced by selective breeding for quantitative agglutinin responsiveness to flagellar (f) or somatic (s) antigens (Ags) ofSalmonellae: high (H) or low (L) responder lines to f Ag and H and L responder lines to sAg.TheSalmonellaecontained both f and sAgs, the Ag used to perform the selection was the Selection Ag and the other was the Associated Ag. The selective breeding produced a progressive interline separation with an equivalent effect for both Ags. After 15 generations (F15) the level of agglutinin response was about 60 times higher in H than in L responders. About 50 % of the phenotypic variation of the character investigated is determined by a group of immune response genes, the rest is due to environmental factors. The nonspecific effect of this group of immune response genes was investigated by measuring the responses to three independent antigens: Sheep erythrocytes (SE), dinitrophenyl‐conjugated human IgG (DNP‐HGG) and bovine IgG (BGG).The selection for fAg response produced an equivalent modification in the responsiveness to the Associated Ag (97 %) and to BGG (130 %). This nonspecific effect was smaller for responsiveness to SE and DNP‐HGG, 58 % and 41 % of the Selection Ag response, respectively.The selection for sAg response produced a nonspecific modification of responsiveness of 94 % for the Associated Ag of 74 % for BGG and 63 % for DNP‐HGG. An important exception concerned SE to which an equal antibody response is produced in high and low lines of sAg se

ISSN:0014-2980    

DOI:10.1002/eji.1830070402

出版商:WILEY‐VCH Verlag GmbH    

年代:1977

数据来源: WILEY

摘要:

AbstractSequential treatment of chicken lymphocytes with normal sheep serum (NSS), chicken antiserum against sheep erythrocytes (SE) and formaldehyde results in rosette formation with SE. The identity of reactive components and the mechanism of rosette formation has been elucidated. The binding activity of NSS to chicken lymphocytes was ascribed to “natural” IgM anti‐species antibodies. Subsequently, chicken IgM anti‐SE antibody reacted with sheep IgM bound to the lymphocyte surface, and, following formaldehyde fixation, rosettes were formed by the addition of SE. The reaction of chicken anti‐SE antibody was competitively inhibited with a hog blood group substance suggesting that it had binding specificity for a carbohydrate moiety which is shared by sheep IgM and SE. This specificity represented only a fraction of the bulk of chicken IgM anti‐SE antibodies and was not manifested by antibodies of the IgG class. The analysis by the above‐described “sandwich rosette assay” was extended to chicken antisera against horse, rat and mouse red cells and to normal sera from these species. Cross‐matching of chicken antisera, with the normal sera and red cells from the tested species revealed a distinct pattern of cross‐reactivities. These results imply that the saccharide epitope which is shared by IgM and red cells has a wide distribution between various mammalian species and may be classified as a new system of

ISSN:0014-2980    

DOI:10.1002/eji.1830070403

出版商:WILEY‐VCH Verlag GmbH    

年代:1977

数据来源: WILEY

摘要:

AbstractThe two mouse immunoglobulin receptors on lymphocytes (IgM and IgD‐like) were individually digested by trypsin. Their tryptic susceptibility, and the products released, were similar to those of their human counterparts. Evidence for a structural homology between human IgD and its presumed mouse counterpart has been provided by the remarkably similar profile of fragments resulting from digestion. More definitive homology awaits sequence determination. The extreme susceptibility of surface IgD to proteolysis contrasted with the resistance of surface IgM. We therefore propose that the major role of IgD is to release a fragment (Fabδ) following exposure to antigen and then elicit a regulatory anti‐idiotype response which acts through recognition of the protease‐resistant IgM idiotype remaining on the cell s

ISSN:0014-2980    

DOI:10.1002/eji.1830070404

出版商:WILEY‐VCH Verlag GmbH    

年代:1977

数据来源: WILEY

摘要:

AbstractExperimental allergic neuritis (EAN) in the peripheral nervous system, without involvement of the central nervous system, was produced in laboratory animals by the injection of a basic neuritogenic protein, P1L, purified from human peripheral nerves. The animals manifested a positive skin test with P1L, and their lymphocytes were found to be transformed in vitro in the presence of this protein several days before the appearance of the clinical signs. Passive transfer of the disease was performed with lymph node cells from donor guinea pigs immunized with P1L protein. EAN, the experimental model for the human disease Guillain‐Barré syndrome, was shown to be a transient disease and could be suppressed by the administration of hydrocortiso

ISSN:0014-2980    

DOI:10.1002/eji.1830070405

出版商:WILEY‐VCH Verlag GmbH    

年代:1977

数据来源: WILEY

摘要:

AbstractPicogram quantities of dinitrophenylated (DNP) dextran, a thymus‐inde‐pendent antigen or lipopolysaccharide, a B cell mitogen, signal down B lymphocytes to unresponsiveness. Down signals were detected by a decision test in which signaled lymphocytes were allowed one hour to react to an immunogenic pulse of DNP‐dextran. Depletion of T cells or macrophages did not interfere with the generation of down signals. The signaling can proceed entirely at 4 °C and its negative effect is transitory. Down signals which could be detected after 15 min at 37°C were reversed by 60 min. It is suggested that down regulation by low dose antigenic signals provides a means of distinguishing background noise from true antigenic

ISSN:0014-2980    

DOI:10.1002/eji.1830070406

出版商:WILEY‐VCH Verlag GmbH    

年代:1977

数据来源: WILEY

摘要:

AbstractDelayed‐type hypersensitivity (DTH) to 2‐phenyl‐4‐ethoxymethylene‐5‐oxazolone (oxazolone) was found to be under multigenic control in inbred, H‐2 congenic and intra‐H‐2 recombinant strains of mice. A high response was associated with haplotypes H‐2d,a,kand low response with haplotype H‐2b.DTH to oxazolone was high or intermediate in different F1hybrids of high and low responder mice. In F2and backcross generations a higher response was associated with the “dd”, than with the “bb” phenotype, while intermediate response was found in the heterozygote “db” mice.A study of H‐2 recombinant strains suggests that a gene controlling the DTH response maps in the I‐B subregion of the H‐2 complex. The response was significantly modified by gene(s) which are not linked to the H‐2 complex and have not been mapped.Since congenitally athymic nude (nu/nu) mice did not respond to oxazolone, this contact sensitivity is belived

ISSN:0014-2980    

DOI:10.1002/eji.1830070407

出版商:WILEY‐VCH Verlag GmbH    

年代:1977

数据来源: WILEY

摘要:

AbstractPreleukemic cells could be detected in the bone marrow cell population of SJL/J mice within several days after induction of leukemia by repeated feedings with a chemical carcinogen 7,12‐dimethylbenz[a]anthracene (DMBA). Bone marrow cells collected 7,30 or 60 days following carcinogenic treatment, developed lymphoid tumors upon transplantation into syngeneic irradiated recipients. The incidence of these tumors varied between 40–45 % when the bone marrow cells were collected and transferred 7–30 days after feeding with DMBA, and raised to an incidence of 80 % when transferred 60 days after carcinogen administration (compared to 50 % incidence in the DMBA‐treated bone marrow donors). A survey of several cell surface components on the lymphoid tumor cells, obtained after transplantation of preleukemic cells, indicated that most of the tumor lines bore both the Thy‐1.2 antigen (weak) and the Fc receptor, whereas the rest were positive only for the Fc receptor. None of these tumor cell lines would yield a significant amount of cell‐bound imm

ISSN:0014-2980    

DOI:10.1002/eji.1830070408

出版商:WILEY‐VCH Verlag GmbH    

年代:1977

数据来源: WILEY

摘要:

AbstractIn the culture medium of some human lymphoblastoid cell lines material is released with the following properties: (a) hemagglutination reaction of IgG‐sensitized erythrocytes; (b) enhancement of precipitation of DNA‐anti‐DNA complexes; (c) inhibition of binding of Clq to immune complexes; (d) inhibition of immune complex binding to lymphocytes; (e) inhibition of antibody‐dependent lymphocytotoxicity The material is not identical with Clq or rheumatoid factor, it is heat resistant (30 min at 56 °C); the molecular weight is about 100 000 daltons and it is capable of inhibiting antibody productionin vitroIt is suggested that this material consists of Fc receptors spontaneously shed from lymphocyte m

ISSN:0014-2980    

DOI:10.1002/eji.1830070409

出版商:WILEY‐VCH Verlag GmbH    

年代:1977

数据来源: WILEY

摘要:

AbstractChicken spleen and bursa cells were examined for the percentage of Fc receptor‐bearing cells. Rosette formation was done with chicken 7S antibody‐sensitized sheep erythrocytes and was inhibited by heat‐aggregated chicken Ig. In the spleen, the percentage was found to increase with age to ∼ 26 % at 7 to 12 weeks. In contrast, only 3 to 5 % of bursa cells at this age demonstrated Fc receptors. Spleens from bursectomized chickens had 7–10 % Fc receptor‐bearing cells.In an attempt to determine a possible role of the C3 receptor on migration patterns, the effect of cobra venom factor (CVF) on the localization of transferred lymphoid cells was examined. Pretreatment of recipients with enough CVF to lower mean C3 levels to 11 % of controls failed to affect follicular B cell localization in mice at either 24 or 48 h after transfer. Localization of thymus or bursa cells in chickens was similarly unaffected by CVF pretreatment.The possible roles of Fc and C3 receptors on migration of B lymphocytes into follicles and germinal centers wer

ISSN:0014-2980    

DOI:10.1002/eji.1830070410

出版商:WILEY‐VCH Verlag GmbH    

年代:1977

数据来源: WILEY

摘要:

AbstractLymphoid cell surface alloantigens were detected by a triple‐layer immunofluorescence technique. An antiserum raised against B locus‐incompatible lymphoid cells also reacted with previously undefined differentiation antigens (CA1) which segregated between B locus homozygous (B14/B14) chickens of the same strain as the antiserum donor. CAl‐positive chickens reacted with the antiserum by agglutination of red cells and staining of all peripheral lymphocytes and thymocytes but not bursal cells. Cross‐absorption experiments have demonstrated that antigens expressed by red cells, T cells (both thymic and peripheral) and B cells (peripheral only) respectively, were of distinct spec

ISSN:0014-2980    

DOI:10.1002/eji.1830070411

出版商:WILEY‐VCH Verlag GmbH    

年代:1977

数据来源: WILEY