摘要:

AbstractWhen mice of the A/J strain were immunized with azobenzenearsonate (ABA) directly coupled to a protein, they produced antibodies that share an inherited cross‐reactive idiotype (CRI). In the antigenic determinant that induces CRI, ABA is very probably coupled to tyrosine, and the structure that protrudes from the polypeptide backbone has two benzene rings and a molecular weight of 419. When this same structure is separated from the polypeptide backbone by a spacer of 99 daltons, it induces a different inherited idiotype (ABA‐HOP‐e) in the same strain of mice. Our data suggest that antibodies with the idiotype CRI recognize the terminal benzene ring and the azo group, but do not fit closely around the second ring structure. Antibodies with the idiotype ABA‐HOP‐e have fine specificity for both benzene rings. Both idiotypes are inherited and both are linked to genes controlling constant regions of the hea

ISSN:0014-2980    

DOI:10.1002/eji.1830071202

出版商:WILEY‐VCH Verlag GmbH    

年代:1977

数据来源: WILEY

摘要:

AbstractA human serum thymic factor (SF) stimulated cyclic adenosine‐3′,5′‐mono‐phosphate (cAMP) synthesis in normal mouse thymocytes. Such stimulation was no longer observed when thymocytes were depleted of hydrocortisone (HC)‐sensitive cells. It was concluded that SF selectively stimulates cAMP in HC‐sensitive cells. Furthermore, incubation of thymocytes with SF enlarged the population of HC‐resistant thymocytes.These results suggest that SF might act on HC‐sensitive thymocytes increasing their cellular cAMP level and inducing their transformation in H

ISSN:0014-2980    

DOI:10.1002/eji.1830071203

出版商:WILEY‐VCH Verlag GmbH    

年代:1977

数据来源: WILEY

摘要:

AbstractTwenty‐five to 30 % of blood lymphocytes from a myeloma patient with an IgGl(k) protein with antibody activity to horse α2‐macroglobulin (α2M) reacted with purified IgG from a rabbit antiserum to the idiotypic determinants of this protein. A small proportion of these idiotype‐bearing lymphocytes were IgG‐bearing B cells, but most of them were T cells. Several experiments demonstrated the actual synthesis of the idiotypic structures carried by the T lymphocytes. All idiotype‐bearing lymphocytes bound horse α2M, and the structures that bore the idiotype and bound the antigen were shown to completely cocap. After biosynthetic labeling with14C‐labeled amino acids, immunoprecipitation and sodium dodecyl sulfate gel electrophoresis, the anti‐idiotypic serum and the α2M antigen were found to react with the same molecules. Preliminary data on the size of these antigen‐binding T lymphocyte‐derived molecules are in accord with those of Binz and Wigzell (Scand. J. Immunol.1976. 5: 559) in the rat. The finding in this patient with multiple myeloma of a homogeneous and possibly malignant population of T lymphocytes, synthesizing identical antigen receptors which share idiotypic (but not isotypic) determinants of the immunoglobulin molecule produced by the malignant B cell clone, therefore confirms in man the results of several recent studies in ot

ISSN:0014-2980    

DOI:10.1002/eji.1830071204

出版商:WILEY‐VCH Verlag GmbH    

年代:1977

数据来源: WILEY

摘要:

AbstractThe specificity of individual clones of cytotoxic lymphocytes (CL) which develop upon stimulation by semi‐allogeneic cells, have been examined and compared with the specificity of CL clones which develop spontaneously when normal spleen cells are cultured without stimulator cells. It was found that the specificity of stimulated clones was different from the specificity of ‘spontaneous’ clones. Specifically stimulated clones from CBA cells cultured with (CBA × DBA)F1stimulator cells, did not discriminate between the H2dP81 5 and H2dDBA splenic blasts, and lysed both targets. In contrast, spontaneous CL clones from cultures of CBA or (CBA × DBA)Fi spleen cells were found to lyse either P8 15 or DBA blasts, but not both. The results indicated that the spontaneous CL clones were not a representative sample of the total poo

ISSN:0014-2980    

DOI:10.1002/eji.1830071205

出版商:WILEY‐VCH Verlag GmbH    

年代:1977

数据来源: WILEY

摘要:

AbstractWhen normal spleen cells are cultured for 4 days in polyacrylamide vessels, individual clones of cytotoxic lymphocytes (CL) can be detected. The specificity of these ‘spontaneous’ CL was investigated by assaying the cytotoxic activity of cells from individual clones against pairs of different targets. Target cells used were syngeneic blast cells induced by dextran sulfate, lipopolysaccharide (LPS), concanavalin A (ConA) and phytohemagglutinin (PHA). It was found that LPS blasts were lysed by a separate set of CL clones from those which lysed PHA blasts of the same H‐2 haplotype, and the clones of CL which lysed PHA blasts were a subset of all the clones which lysed ConA blasts. When individual clones of spontaneous CL were assayed against LPS and DS blasts, there were clones which lysed both types of blasts as well as clones which were specific for either LPS or DS blasts. These results have been interpreted as demonstrating that spontaneous CL can recognize and kill subsets of cells which are stimulated by different mit

ISSN:0014-2980    

DOI:10.1002/eji.1830071206

出版商:WILEY‐VCH Verlag GmbH    

年代:1977

数据来源: WILEY

摘要:

Abstract2,4,6‐Trinitrobenzene sulfonic acid (TNBS) injected intravenously (i.v.) makes mice fully tolerant to the trinitrophenyl (TNP) determinant. Administration ofin vitroTNP‐labeled syngeneic erythrocytes or thymocytes renders mice unable to develop contact sensitivity to picryl chloride, while the humoral anti‐TNP responses seem to be unaffected. The reverse was found after pre‐treatment of mice with TNP‐labeled isologous IgG (MGG) since only anti‐TNP antibody responses, but not contact sensitivity to picryl chloride, were significantly reduced. TNP‐coupled macrophages given to animals suppressed both the cell‐mediated and humoral responses, and this might be due to the presence on their surface of TNP‐labeled cytophilic antibody. TNBS administered i.v. binds to circulating proteins and formed blood elements. Thus the split unresponsiveness affecting either humoral or cell‐mediated compartments after the injection of TNP‐MGG or of haptenated cells respectively, is presumably due to dissecting events whichin vivoafter the injection of TNBS, occur simultaneously. These results may be interpreted to indicate that split unresponsive states to TNP determinants are mediated by two independent mechanisms which require different tolerogen presenta

ISSN:0014-2980    

DOI:10.1002/eji.1830071207

出版商:WILEY‐VCH Verlag GmbH    

年代:1977

数据来源: WILEY

摘要:

AbstractSurface immunoglobulin allotypes on rabbit perpheral bloodlymphocytes (PBL)and spleen lymphocytes are detected by an immunoferritin labeling technique for electron microscopy. Human red blood cells, chemically coated with purified rabbit lgG of specific allotypes, served as test cells to assess the labeling speecificity and efficiency. Immunoelectron microscopic labeling reveals that groups a,b,d and e allotypic specificities are readily detectable on passively coated test cells. However, only a and b group markers are detectable on lymphocytes. On rabbit PBL both a (VHof Fd reguib) abd b (kappa chain constant region);group alloytpes are detectable (73‐78% and 68‐77%, respectively); on spleen cells, 46% and 52‐55%are positive for a and b locus allotypes, respectively.The d and e allotypes (i.e.γ chain‐specific) are undetectable on PBL or on spleen lllllymphocytes using this method. We conclude that the d and e group allotypes are either not present on the lymphocyte surface or are buried in the surface memberane and unaccessible to the antisera used in this immunoferritin labeling t

ISSN:0014-2980    

DOI:10.1002/eji.1830071208

出版商:WILEY‐VCH Verlag GmbH    

年代:1977

数据来源: WILEY

摘要:

AbstractA B10.A(5R) responder mouse to the random linear terpolymer, poly‐L‐(Glu, Lys, Phe), GLΦ, can produce immunoglobulins which bind poly‐L (Glu, Lys), GL, that share idiotypic determinants with GL‐ binding antibodies produced by other members of the same strain. Expression of these common idiotypic determinants, termed BGL, is independent of the H‐2 haplotype and closely linked to the Ig‐1bheavy chain allotype. Moreover, nonresponder mice with the Ig‐1bheavy chain allotype, when immunized with GLΦ that has been chemically coupled to an immunogenic carrier, chicken IgG, can produce GL‐binding antibodies that share BGL idiotypic specificities with anti‐GLΦ antibodies produced by responder animals. Also, the responses to other GL‐containing polymers, such as poly‐L (Glu, Lys, Ala) and poly‐L (Glu, Lys, Pro), which are under the control of distinct Ir genes, can stimulate the production of GL‐binding antibodies that share common BGL idiotypic determinants with antibodies induced with GLΦ. These findings are discussed with respect to their implications concerning the me

ISSN:0014-2980    

DOI:10.1002/eji.1830071209

出版商:WILEY‐VCH Verlag GmbH    

年代:1977

数据来源: WILEY

摘要:

AbstractGraft rejection in the earthworm,Lumbricus terrestris, is mediated by granulocytic coelomocytes (neutrophils, by light microscopy). Within 1–3 days post‐transplantation of body‐wall xenografts, granulocytic coelomocytes migrate to the transplant site, penetrate the graft matrix, and actively phagocytize viable muscle fibers. Destruction of muscle tissues may be augmented by lymphocytic coelomocytes (basophils, by light microscopy), but these cells participate in a minor way and do so very late in the rejection process (days 11‐13). Xenografts, autografts and sham‐operated controls display generalized inflammatory reactions, including coelomocyte infiltration into the grafted tissue. In autografts, however, granulocytes only penetrate the inner longitudinal muscle layers and extensive infiltration, persistence and complete destruction never occur. Lymphocytic coelomocytes are not observed in autografts or sham‐operated controls. Graft rejection by leukocytes in an advanced invertebrate confirms that aspects of cell‐mediated immunity evolved early in phyloge

ISSN:0014-2980    

DOI:10.1002/eji.1830071210

出版商:WILEY‐VCH Verlag GmbH    

年代:1977

数据来源: WILEY

摘要:

AbstractThe effect of neonatal anti‐idiotypic suppression on the expression of B cells of the T15 clonotype has been investigated at the level of individual clonal precursor cells. The results indicate that B cells of the T15 clonotype are almost completely eliminated from the repertoire for four months after neonatal injection of allogeneic anti‐idiotypic serum. The degree of this suppression is dependent on the amount of anti‐idiotypic antibody administered and is less profound if anti‐idiotypic antibody is given after the first week of life. No suppression was observed when anti‐idiotypic antisera were administered to mice 30 days of age or older, which may indicate that immature B cells are the population most susceptible to suppression. However, since suppression could be reversed by administration of T15 myeloma protein several days after injection of anti‐idiotype, the inability to suppress adult BALB/c mice may have been due to the high level of T15 idiotype normally present in their serum. Finally, phosphorylcholine‐responsive B cells of identifiable clonotypes other than T15, even a clonotype sharing antigen‐combining site determinants with T15, appear unaffected by anti

ISSN:0014-2980    

DOI:10.1002/eji.1830071211

出版商:WILEY‐VCH Verlag GmbH    

年代:1977

数据来源: WILEY