摘要:

AbstractHighly purified murine lymph node T cells were used to test the hypothesis that polyclonal T cell activation requires the recognition of mitogen‐modified major histocompatibility complex (MHC) antigens on accessory cells (AC) by the T cells. A veriety of tumor cell lines, including macrophage, B and mast cell tumors, as well as thymomas, were shown to function as AC in concanavalin A‐induced T cell activation, even if they expressed only one class of MHC antigens or none at all. In contrast to antigen‐specific responses, where the Lyt‐2+phenotype is reportedly associated with recognition of class I MHC antigens, T cells enriched for or depleted of Lyt‐2+cells were not preferentially activated in the presence of class I‐ or class II‐positive AC, respectively.In addition, as shown by others in the guinea pig and in the rat systems, T cell proliferation induced by oxidation of cell surface sugars is equally effective if T cells or AC are oxidized. T cell mitogens, therefore, do not seem to act by altering MHC antigens on AC, but rather by providing T cell‐AC contact via their agglutina

ISSN:0014-2980    

DOI:10.1002/eji.1830140602

出版商:WILEY‐VCH Verlag GmbH    

年代:1984

数据来源: WILEY

摘要:

AbstractIn the present study, interleukin 1 (IL 1)‐containing media from different sources, namely a murine macrophage cell line (P388D1), rabbit peritoneal macrophages, and human peripheral blood mononuclear cells, were compared for their effect on thymocyte proliferation and on collagenase and PGE2secretion by chondrocytes. A high correlation was found between the enhancement of thymocyte proliferation and the induction of collagenase and PGE2secretion by chondrocytes. Furthermore, a highly purified IL 1‐like factor, namely mononuclear cell factor (MCF) was also active on chondrocytes. The addition of highly purified IL 2 to rabbit chondrocytes had no effect on collagenase and PGE2secretion induced by IL 1‐containing media. Our findings suggest that the factor which induced collagenase and PGE2secretion by rabbit chondrocytes was an IL 1‐like factor. Thus, collagenase secretion by chondrocytes may be used as an IL 2‐insensitive assay for the detection of IL 1‐l

ISSN:0014-2980    

DOI:10.1002/eji.1830140603

出版商:WILEY‐VCH Verlag GmbH    

年代:1984

数据来源: WILEY

摘要:

AbstractMouse bone marrow cells exposed to fluorescein‐conjugated peanut agglutinin (PNA) showed subsets of highly labeled cells when analyzed in a fluorescence‐activated cell sorter. After separating three cell fractions of large and small PNA‐binding cells and PNA‐nonbinding cells, respectively, the B lymphocyte precursor (pre‐B) cells, having cytoplasmic μ chains (cμ) without surface μ chains (sμ), were recovered solely in the PNA‐binding fractions. Only a minority of sμ+small lymphocytes having the lowest densities of sμ bound PNA. Small and large cμ+sμ−pre‐B cell populations were separated in high degrees of purity in the PNA‐binding fractions, especially when obtained from bone marrow undergoing lymphoid regeneration after sublethal X‐irradiation and during stimulation of lymphocyte production in post‐polycythemic erythroid suppression. Characteristic shifts in the size distribution profile of PNA‐binding cells reflected changes in the maturation stage of the pre‐B cells. The results demonstrate that surface membrane components with strong PNA‐binding capacities characterize cμ+sμ−pre‐B cells in the bone marrow during both normal and perturbed primary B lymphocyte genesis. The PNA‐binding sites become undetectable soon after the first expression of sμ. This property permits the isolation from the bone marrow of high concentrations of subsets of large and small cμ+sμ−cells in a viable stat

ISSN:0014-2980    

DOI:10.1002/eji.1830140604

出版商:WILEY‐VCH Verlag GmbH    

年代:1984

数据来源: WILEY

摘要:

AbstractEight monoclonal anti‐idiotypic antibodies directed against public idiotopes have been further characterized: (a) they bind to public idiotopes with a high affinity; (b) they recognize all anti‐poly(Glu60, Ala30, Tyr10) (GAT) antibodies as measured by inhibition of the anti‐GAT plaque‐forming cell response. This has been verified in three strains of mice. These reagents were not able to detect idiotope expression on eight GAT‐specific helper T cell lines and clones. This result was obtained by two techniques: (a) idiotope expression at the T cell surface was measured by indirect immunofluorescence using a cell sorter with surface antigens H‐2D, Thy‐1.2, Lyt‐1 and L3T4 as positive controls; (b) after immunoadsorption of [35S]methionine‐labeled cellular extracts from two lines, no unique molecule was retained by the HP‐idp22 monoclonal anti‐idiotypic antibody coupled to Sepharose. Despite these negative results, this antibody was found to prime lymph node cellsin vivo, which were able to proliferate specifically in response to GAT. Two T cell lines derived from this lymphocyte population do not express any of the idiotopes tested. These results suggest that monoclonal anti‐idiotypic antibodies may be influencing T lymphoc

ISSN:0014-2980    

DOI:10.1002/eji.1830140605

出版商:WILEY‐VCH Verlag GmbH    

年代:1984

数据来源: WILEY

摘要:

AbstractThe proteins p40 (Mr= 40000), p32 (Mr= 32000), p28 (Mr= 28000), p20 (Mr= 20000) and p10 (Mr= 10000) are described which occur in noncovalent association with the polymorphic α,β heterodimer of class II antigens. They were investigated with respect to their molecular characteristics and their mutual structural relationship. p32, the predominant species of this group corresponds to the invariant chain γ (Ii). In contrast to the polymorphic subunits α and β, proteins p40, p28, p20 and p10 migrated like γ in electrophoretically constant positions, when class II molecules of different subregions and different alleles were assessed by two‐dimensional gel electrophoresis [1st dimension, isoelectric focusing; 2nd dimension, sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis]. Analogous to γ, they are therefore designated invariant chains. The low Mrspecies of this group do not arise from higher Mrforms as preparation artefacts. Short‐term pulse‐chase analysis and cell‐free translation of sucrose gradient‐fractionated mRNA in conjunction with specific immunoprecipitation rendered the possibility unlikely that individual components of this set of proteins existed in a precursor‐product relationship within the cell. Comparative enzymatic fragmentation on SDS polyacrylamide gels as well as tryptic peptide map comparisons by high performance liquid chromatography revealed a high structural relatedness among all members of this group of

ISSN:0014-2980    

DOI:10.1002/eji.1830140606

出版商:WILEY‐VCH Verlag GmbH    

年代:1984

数据来源: WILEY

摘要:

AbstractA monoclonal antibody, designated CLB‐LFA‐1/1, directed to the human lymphocyte‐function‐associated antigen 1 (LFA‐1) was raised by immunization of mice with the peripheral blood lymphocytes of a Tγ lymphocytosis patient. The monoclonal antibody was selected by inhibition of the natural killer cell and the antibody‐dependent killer cell activity of the patient's Tγ lymphocytes. In addition, the monoclonal antibody was shown to inhibit the cytotoxic activity of T cell clones specific for either class I or class II HLA molecules. The antigen recognized by CLB‐LFA‐1/1 consisted of three polypeptide chains with molecular weights of 180000 (α), 155000 and 94000 (β). The antibody reacted with T cells, B cells, monocytes and granulocytes, and stained normal Tγ cells and Tγ cells of patients with Tγ lymphocytosis two‐ to threefold stronger than normal T cells. It was shown that LFA‐1 and the Fc receptor on Tγ cells did not comodulate and it is therefore concluded that Fc receptors and LFA‐1 are independent membrane structures, both required for the kil

ISSN:0014-2980    

DOI:10.1002/eji.1830140607

出版商:WILEY‐VCH Verlag GmbH    

年代:1984

数据来源: WILEY

摘要:

AbstractSeven hybridoma cell lines secreting monoclonal antibodies (mAb) to nonlymphoid cells of the mouse thymus have been prepared. These mAb clearly demonstrate the heterogeneity of the thymic stroma. Based on their anatomical distribution patterns observed with the immunoperoxidase technique on frozen tissue sections, they were subdivided into four groups. The first group of mAb, ER‐TR1, 2 and 3, detects antigens encoded for by the I region of the major histocompatibility complex. These antigens are expressed on both stromal and lymphoid cells in lymphoid organs. mAb of the second category, ER‐TR4, react with epithelial cells in the thymic cortex. mAb of the third group detect stromal cells of the thymic medulla. One antibody of this group, ER‐TR5, exclusively reacts with medullary epithelial cells. ER‐TR6, the other antibody of this group, reacts with medullary interdigitating cells and macrophages. The fourth type of antibodies, ER‐TR7, detects the reticular fibroblasts of the thymus. The possible role of the thymic cell types detected by the present antibodies in T cell differentiation is

ISSN:0014-2980    

DOI:10.1002/eji.1830140608

出版商:WILEY‐VCH Verlag GmbH    

年代:1984

数据来源: WILEY

摘要:

AbstractBALB/c mice immunized with the phospholipid, cardiolipin, produced anti‐cardiolipin and anti‐DNA antibodies. Seven hybridomas derived from spleen cells of the cardiolipin‐immunized mice produced cardiolipin‐binding monoclonal antibodies that also bound to the polynucleotides DNA, poly(dT), and poly(I). The seven cardiolipin‐induced monoclonal antibodies shared idiotypic determinants with a high frequency idiotypic marker of spontaneously expressed anti‐DNA autoantibodies of lupus‐prone MRL‐lpr/lprmice. The monoclonal antibodies presumably bound to phosphodiester phosphate groups that occur in both polynucleotides and phospholipids. The results imply that production of anti‐DNA autoantibodies does not require im

ISSN:0014-2980    

DOI:10.1002/eji.1830140609

出版商:WILEY‐VCH Verlag GmbH    

年代:1984

数据来源: WILEY

摘要:

AbstractSeventeen monoclonal rat antibodies with specificities for mouse μ heavy chain recognize seven distinguishable determinants that are located on the four constant region domains. All determinants are present on secreted, intracellular and membrane bound IgM, and all but two are expressed on isolated μ heavy chain. One antibody, specific for a site in the first constant region domain, recognizes a determinant that is present on IgM of AKR, C3H/HeJ, C57BL/6J, SJL, DBA/2, BALB/c, NZB and CBA/J mouse strains, but not on IgM of A.TH, A/J and A.CA strains of mic

ISSN:0014-2980    

DOI:10.1002/eji.1830140610

出版商:WILEY‐VCH Verlag GmbH    

年代:1984

数据来源: WILEY

摘要:

AbstractIn a previous report (M. Barel et al.FEBS Lett., 1981.136:111) using radiolabeling methods, we characterized from the membrane of the human B lymphoblastoid cell line Raji, a 140000‐Mrglycoprotein (gp140) carrying a C3b‐binding activity with125I‐labeled C3b or Sepharose‐bound C3b. The facts of absence on Raji cells of CR1, the C3b receptor purified from human erythrocytes, the observations made by others that H‐like activity (the 150000 MrC3b binding serum protein) was present in Raji cells and the same molecular weight range of H and gp140, led us to investigate the relationship between both antigens.A rabbit antibody anti‐5.4 was prepared against gp140, highly purified from Raji cells. However, anti‐H specificities were detected in crude anti‐5.4 IgG, while anti‐serum H IgG did not react with gp140 antigen. The crude anti‐5.4 IgG fraction, anti‐gp140 IgG or F(ab′)2and anti‐H specificities present in anti‐5.4 IgG, separated by absorption on Sepharose‐bound H, and anti‐serum H IgG were tested on Raji cells by immunofluorescence techniques, by measuring the inhibition of specific cytotoxic assays and the inhibition of specific binding of soluble or particle‐bound C3b to the cell surface and on solubilized antigens by immunoblotting techniques. All the data obtained support that: (a) anti‐H specificities are not shared by antibodies bearing anti‐gp140 specificities and their presence in crude anti‐5.4 IgG is more likely due to a contamination by H antigen of gp140 antigen used in the immunization process, and (b) gp140 antigen is highly expressed on Raji cell surface, whereas H antigen can not be detected under the same conditions. Molecular analysis of gp140 and H antigens confirmed differences between both antigens in molecular weight, trypsin sensitivity and charge properties.All the results presented herein support the notion that gp140 is not identical with the H molecule and that C3b binding to gp140 is not mediated by H. The relationship between gp140 and C3 rec

ISSN:0014-2980    

DOI:10.1002/eji.1830140611

出版商:WILEY‐VCH Verlag GmbH    

年代:1984

数据来源: WILEY