摘要:

SummaryHuman T lymphocyte colony formation can be induced in a two‐step culture system with phytohaemagglutinin (PHA) as the only exogenous growth factor. In this system the first step is the overnight pre‐incubation of mononuclear cells in a liquid medium in the presence of PHA (‘activation step’). The second step is the seeding of these PHA‐activated cells into semi‐solid agar, again in the presence of PHA, and their culture for 7 days (‘proliferative step’). We have previously proposed that interleukin 2 (IL2) is produced during the second (proliferative) step and that this IL2 facilitates the production of colonies from PHA‐activated colony‐forming cells. Here we substantiate this proposal by demonstrating that both highly purified IL2 and recombinant IL2 stimulate T colony formation from PHA‐treated cells. The monoclonal antibody anti‐Tac, which is specifically directed against the IL2 receptor, dramatically inhibited colony formation when it was included during the agar culture (proliferative step). There was no effect when anti‐Tac was included during the liquid pre‐incubation culture (activation step). These results provide strong evidence that IL2 is the critical factor in the development of T lymphocyte colonies at the stage of conversion of PHA‐activated cells into colonies.

ISSN:0004-945X    

DOI:10.1038/icb.1987.11

出版商:Nature Publishing Group    

年代:1987

数据来源: WILEY

摘要:

SummaryAn antigen‐capture enzyme‐linked immunosorbent assay (ELISA) has been developed to detect antigens of Australian flaviviruses in mosquito pools, suckling mouse brain and infected cell culture supernatant fluid. A monoclonal antibody reactive to an epitope on the envelope glycoprotein common to all flaviviruses was used as the capture antibody. Purified rabbit IgG, produced against Murray Valley encephalitis (MVE) virus, which reacted with eight Australian flaviviruses in haemagglutination inhibition (HI) and in an indirect fluorescent antibody test, was used as the indicator antibody in direct and indirect antigen‐capture ELISA. A monoclonal antibody specific for a subgroup of encephalitic flaviviruses was conjugated to horseradish peroxidase and used as the indicator antibody to distinguish MVE, Kunjin and Alfuy viruses from the remainder tested. This ELISA could detect viral antigen in mosquito cell culture fluids and suckling mouse brain preparations at titres as low as 1000 TCID50/100μ1. Viral antigen in a single mosquito infected with MVE could be detected in a pool of 500.

ISSN:0004-945X    

DOI:10.1038/icb.1987.12

出版商:Nature Publishing Group    

年代:1987

数据来源: WILEY