摘要:

SummaryOf 12 brush‐tailed possums(Trichosurus vulpecula)inoculated withLeptospira interrogansserovarbalcanica11 developed migroagglutination (MA) antibody tohardjoantigen by 14 days postinoculation (PI). Leptospiruria was observed in 2 possums 117 to 145 days PI. Of 6 possums inoculated with serovarhardjo4 developed low short‐lived titres by day 18 PI. Two of 3 wombats(Vombatus ursinus)inoculated withbalcanicahad high MA titres (≥ 1:128) by day 16 PI and leptospiruria occurred by day 16. One wombat inoculated withhardjodeveloped a low MA titre. Low transitory MA titres tohardjowere found in 1 of 3 water rats(Hydromys chrysogaster)after inoculation withbalcanicaand 1 of 2 givenhardjo. Histopathological examination of kidneys revealed mild to moderately severe focal interstitial nephritis in 4 of 8 possums, in 2 wombats and in 2 water rats following experimental infection withbalcanica. Similar lesions were observed in 2 of 4 possums, 1 wombat and 2 water rats following experimental infection withhardjo.

ISSN:0004-945X    

DOI:10.1038/icb.1979.24

出版商:Nature Publishing Group    

年代:1979

数据来源: WILEY

ISSN:0004-945X    

DOI:10.1038/icb.1979.25

出版商:Nature Publishing Group    

年代:1979

数据来源: WILEY

摘要:

SummaryThe two antiproteases α1‐antitrypsin (α1‐AT) and antithrombin‐III (AT‐III) have been purified simultaneously from human plasma. Purification procedure consisted of gel filtration on Sephadex G‐200 after initial processing of plasma, followed by ion exchange chromatography on DEAE‐Sephadex A50 and DEAE‐celllulose, at a pH of 9·0 and pH 8·3 respectively. The two proteins could not be separated by any of these procedures including a lower pH (7·4) in ion exchange chromatography. Affinity chromatography on heparin‐Sepharose separated the proteins since α1‐AT did not bind to the matrix. Alpha1‐AT unbound to the heparin‐Sepharose was subsequently purified through con A‐Sepharose affinity column. The final yield of both the proteins was about 20%. The molecular weight estimated on SDS electrophoresis for AT‐III and α1‐AT was 63,000 and 50,000, respectively.

ISSN:0004-945X    

DOI:10.1038/icb.1979.26

出版商:Nature Publishing Group    

年代:1979

数据来源: WILEY

摘要:

SummaryUrinary calculi composed of calcium oxalate were produced in male hooded Wistar rats fed a vitamin B6deficient diet over 16 weeks. This basic diet was modified by doubling the phosphate content or loading with vitamin C or D3in three treatment groups. The number of rats developing oxalate stones was not altered by the addition of vitamin D3or phosphate, but there was a significant increase in total weight of stone formed and histological evidence of extensive renal damage in rats on the high vitamin D3diet. The addition of vitamin C to the vitamin B6deficient rats resulted in a reduction in the number of rats with uroliths and a fall in urinary oxalate excretion, while similarly loaded vitamin B6supplemented controls were free of oxalate calculi.It is concluded that the oxalate urolithiasis induced by vitamin B6deficiency was exacerbated by added vitamin D3and reduced by vitamin C.

ISSN:0004-945X    

DOI:10.1038/icb.1979.27

出版商:Nature Publishing Group    

年代:1979

数据来源: WILEY

ISSN:0004-945X    

DOI:10.1038/icb.1979.28

出版商:Nature Publishing Group    

年代:1979

数据来源: WILEY

ISSN:0004-945X    

DOI:10.1038/icb.1979.29

出版商:Nature Publishing Group    

年代:1979

数据来源: WILEY

摘要:

SummarySpecific IgG antibody given intravenously 3–4 hours prior to oral immunisation withVibrio choleraeled to a specific depression of both the systemic and local immune response. One vibriocidal unit of IgG antibody, which itself would give undetectable levels of circulating specific antibody, was significantly immunosuppresive. The suppression is considered to be due to central repression of antigen‐reactive lymphocyte, rather than to antigen exclusion at the gut mucosal surface. The repression appeared less pronounced in some immunoglobulin classes than in others.

ISSN:0004-945X    

DOI:10.1038/icb.1979.30

出版商:Nature Publishing Group    

年代:1979

数据来源: WILEY

摘要:

SummaryThe clearance of organisms from the lungs of mice was followed after aerosol administration. Preopsonisation of the organisms with immune serum, as a source of specific antibody, enhanced the rate of pulmonary clearance while s.IgA delayed clearance. In the peritoneal cavity, bacteria pre‐treated with immune serum were cleared more rapidly than unopsonised bacteria, but s.IgA had little effect. The presence of Fc receptors for IgG and not s.IgA on alveolar macrophages suggests that, in secretions, IgG is the predominant antibody promoting phagocytosis by alveolar macrophages and that any protective effect of s.IgA is not mediated by these cells.

ISSN:0004-945X    

DOI:10.1038/icb.1979.31

出版商:Nature Publishing Group    

年代:1979

数据来源: WILEY

摘要:

SummaryA prototype immunodiagnostic assay has been developed using chronic infection with the larval cestode,Mesocestoides corti, as a model system in mice. The assay is highly sensitive, it appears to be absolutely specific forM. cortiinfection, and is based on the inhibition of binding (by sera from infected mice) of a radiolabelled anti‐M. cortihybridoma antibody to a crudeM. cortiantigen extract. The hybridoma antibody binds to livingM. cortilarvae and is an IgG1protein. In large scale experiments no false positives were detected and the onlyM. corti‐infected mice not detected by the assay where hypothymic nude (nu/nu) mice. Only limited success has been achieved in attempts to convert the assay to one not requiring parasite antigen and based on the inhibition of binding of radiolabelled anti‐parasite hybridoma antibody and a large pool of anti‐idiotype antiserum. Monoclonal antibodies derived from anti‐parasite antibody‐secreting hybridoma cell lines will be of particular use in the development of new, highly specific, immnunodiagnostic reagents for the detection of parasite infection, exposure and disease.

ISSN:0004-945X    

DOI:10.1038/icb.1979.32

出版商:Nature Publishing Group    

年代:1979

数据来源: WILEY

摘要:

SummaryAffinity chromatography was used to purify rabbit antibodies to common and strain‐specific antigeneic determinants of haemagglutinin, to neuraminidase, and to a combination of the internal proteins of influenza A viruses. The purity of the antibodies was assessed by haemagglutination‐inhibition, enzyme‐linked immunosorbent assay (ELISA) and competition ELISA. The antibodies were examined for their participation in neutralization and haemagglutination‐inhibition assays, and in ELISA. ELISA was found to be the most sensitive technique for detecting antibodies. Antibodies to the common and strain‐specific determinants of haemagglutinin were shown to participate in neutralization assays and ELISA, but only those antibodies to the common determinants were detected by haemagglutination‐inhibition.

ISSN:0004-945X    

DOI:10.1038/icb.1979.33

出版商:Nature Publishing Group    

年代:1979

数据来源: WILEY