ISSN:0004-945X    

DOI:10.1038/icb.1991.41

出版商:Blackwell Publishing Ltd    

年代:2017

数据来源: WILEY

ISSN:0004-945X    

DOI:10.1038/icb.1991.42

出版商:Blackwell Publishing Ltd    

年代:2017

数据来源: WILEY

ISSN:0004-945X    

DOI:10.1038/icb.1991.43

出版商:Blackwell Publishing Ltd    

年代:2017

数据来源: WILEY

ISSN:0004-945X    

DOI:10.1038/icb.1991.44

出版商:Blackwell Publishing Ltd    

年代:2017

数据来源: WILEY

ISSN:0004-945X    

DOI:10.1038/icb.1991.45

出版商:Blackwell Publishing Ltd    

年代:2017

数据来源: WILEY

ISSN:0004-945X    

DOI:10.1038/icb.1991.46

出版商:Blackwell Publishing Ltd    

年代:2017

数据来源: WILEY

ISSN:0004-945X    

DOI:10.1038/icb.1991.47

出版商:Blackwell Publishing Ltd    

年代:2017

数据来源: WILEY

ISSN:0004-945X    

DOI:10.1038/icb.1991.48

出版商:Blackwell Publishing Ltd    

年代:2017

数据来源: WILEY

摘要:

SummaryThe interactions betweenChlamydia trachumatisand human blood mononuclear leukocytes were studied using flow cytometry. Immunofluorescence, electron microscopy and cytokine assays. Under serum‐free conditions, elementary bodies (EB) ofC. trachomatiswere found to bind to human T lymphocytes as well as to B cells and monocytes/macrophages (Mφ). For all cell types the binding was saturable. rapid, temperature‐independent and independent of the chlamydia‐specific serological status of the donor. Similar proportions of T and B cells bound EB at similar levels. In the T cell population, proportionally less CD8+cells bound EB. Whereas Mφ phagocytosed and destroyed the bound micro‐organisms for lymphocytes, theChlamydiaremained at the surface, adherent to morphologically featureless membrane areas and showed no evidence of uptake even after long periods at 37°C. Host molecules modulated these basic binding patterns: a heat‐stable serum factor inhibited EB binding to T cells and a heat‐labile serum factor enhanced binding to B cells. Stimulation withC. trachomatisEB rapidly elicited cytokine production by lymphocytes including interleukin‐6 from B cells and interferon‐gamma (IFN‐γ) from T and/or nonT/nonB cells. The responses were irrespective of the serological status of the donor. The findings suggest thatC. trachomati‐leucocyteinteractions may differ from the interactions of other bacteria and human leucocytes. The possible relationship between leucocyte‐binding, cytokine induction, and the pathognomonic development of lymphoid follicles during mucosalC. trachomatisinfections is discussed.

ISSN:0004-945X    

DOI:10.1038/icb.1991.49

出版商:Blackwell Publishing Ltd    

年代:2017

数据来源: WILEY

摘要:

SummaryT cells are thought to play a fundamental role in the pathogenesis of rheumatoid arthritis (RA), Activated T cells expressing α/β T cell receptor (Tcr) infiltrate the rheumatoid synovium and could potentially initiate a local inflammatory response directed against joint constituents, A Tcr repertoire with restricted heterogeneity may reflect a selective expansion of T cells reactive to a few antigenic determinants within the synovium. To determine whether predominant Vαand/or Vβgene usage of the expressed α/β Tcr repertoire is a feature of synovial T cells in patients with RA, the polymerase chain reaction (PCR) was used to amplify Tcr‐α and Tcr‐β chain transcripts. The peripheral blood and synovia of five patients with adult RA were examined and no evidence of preferential use of 19 Tcr Vα gene families was found. Similarly, most of the 18 Tcr Vβ gene families could be detected in RA synovia although there were quantitative differences in Tcr Vβgene expression when compared to peripheral blood. This report shows that when the extremely sensitive assay of oligonucleotide hybridization of PCR amplified Tcr transcripts is used, permitting identification of specific V gene families, the α/β Tcr repertoire in the rheumatoid synovium is more diverse than was previously thought. Therefore, in patients with RA of long duration, the synovial T cell response is most likely to be polyclonal.

ISSN:0004-945X    

DOI:10.1038/icb.1991.50

出版商:Blackwell Publishing Ltd    

年代:2017

数据来源: WILEY