ISSN:0004-945X    

DOI:10.1038/icb.1992.29

出版商:Blackwell Publishing Ltd    

年代:2017

数据来源: WILEY

ISSN:0004-945X    

DOI:10.1038/icb.1992.21

出版商:Blackwell Publishing Ltd    

年代:2017

数据来源: WILEY

摘要:

SummaryTechnetium‐99 m (99mTc)‐labelled conjugates of an anti‐fibrin monoclonal antibody, DD‐3B6/22, have been assessed for their detection of vascular thrombi in a rabbit model. DD‐3B6/22 binds to a D‐dimer epitope present on cross‐linked fibrin but absent from the fibrin monomer or fibrinogen. Injection of a99mTc‐label led Fab′ fragment of DD‐3H6/22 allowed delineation of model thrombi as early as 30 min postinjection (p.i.) with optimal localization at 4–5 h. Thrombus label uptake at 4 h p.i. was 0.304 ± 0.106% injected dose/g (% ID/g) compared with 0.022 ± 0.001% ID/g after the injection of a control Fab′ fragment. These results suggest that the99mTc‐labelled Fab′ fragment of DD‐3B6/22 has excellent potential for scintigraphic detection of vascular thrombi in humans.

ISSN:0004-945X    

DOI:10.1038/icb.1992.22

出版商:Blackwell Publishing Ltd    

年代:2017

数据来源: WILEY

摘要:

SummaryAn immunoblotting procedure was developed to detect antibody responses in mice and humans to influenza virion proteins. The technique was capable of detecting 1.5 μg of haemagglutinin (HA) on nitrocellulose strips at a 1: 5000 dilution of a mouse serum with an initial haemagglutination inhibition titre of 20. The effects of the use of the blocking agent Tween‐20 on virion proteins were also studied. The commonly used concentration of 0.05% (v/v) Tween‐20, when included in blocking and incubation buffers, greatly reduced the amount of detectable matrix protein but caused no detectable loss of HA and neuraminidase/nucleoprotein proteins. If virion proteins were separated by polyacrylamide gel electrophoresis under reducing conditions, antibody bound to HA2 more strongly than to HA1. Under non‐reducing conditions, more antibody bound to the uncleaved HA protein than to other proteins. IgG1 and IgG2a antibody responses in mice to each protein were stronger than IgG2b and IgG3 responses.

ISSN:0004-945X    

DOI:10.1038/icb.1992.23

出版商:Blackwell Publishing Ltd    

年代:2017

数据来源: WILEY

摘要:

SummaryRetroviruses have been implicated as causative agents for a range of diseases including neoplasia, autoimmunity and immunosuppression. No two retroviruses carry the same complement of genes and for this reason it is not surprising that they induce a variety of different disease states. One common element in retroviral evolution has been the need to avoid immune recognition in order to persist within the host. A comparative approach, looking at various persistent retroviruses, has been used to pin‐point the types of genetic adaptations adopted by retroviruses to remain hidden, often within the T cell compartment. Most of these retroviruses are T‐cell‐tropic and the diseases which they induce usually reflect the effect of the retrovirus on normal lymphocyte function.

ISSN:0004-945X    

DOI:10.1038/icb.1992.24

出版商:Blackwell Publishing Ltd    

年代:2017

数据来源: WILEY

ISSN:0004-945X    

DOI:10.1038/icb.1992.25

出版商:Blackwell Publishing Ltd    

年代:2017

数据来源: WILEY

摘要:

SummaryRecent studies of the structural basis of antigenic variation in influenza virus neuraminidase and of the structure of neuraminidase‐antibody complexes are summarized.

ISSN:0004-945X    

DOI:10.1038/icb.1992.26

出版商:Blackwell Publishing Ltd    

年代:2017

数据来源: WILEY

ISSN:0004-945X    

DOI:10.1038/icb.1992.27

出版商:Blackwell Publishing Ltd    

年代:2017

数据来源: WILEY

摘要:

THE PLATELET IN HEALTH AND DISEASEEdited by Clive P.ASTHMA: ITS PATHOLOGY AND TREATMENTEdited by Michael A. Kaliner, Peter J. Barnes and Karl A. Pearson.ALLERGEN IMMUNOTHERAPY (ALLERGIC DISEASES AND THERAPY SERIES VOL. 4)Edited by R. F. Lockey and S, C. Bukantz.LYMPHATIC TISSUES AND IN VIVO IMMUNE RESPONSESEdited by B. A. Imhof, S. Berrih‐Aknin and S. Ezine.

ISSN:0004-945X    

DOI:10.1038/icb.1992.28

出版商:Blackwell Publishing Ltd    

年代:2017

数据来源: WILEY