摘要:

Because stress proteins are believed to play an important role in cellular repair and survival mechanisms, we investigated accumulation of the 70-kilodalton stress protein (SP70) and its mRNA in the rat corneal epithelium after hyperthermia. In the corneal epithelium of control rats, in situ hydridization with a radioactive probe for SP70 mRNA followed by autoradiography revealed very few silver grains. Eighteen hours after the rats were subjected to hyperthermia, the density of silver grains was greatly increased and this elevated level of corneal epithelium SP70 expression continued through 50 hours after heat treatment. Immunostaining for SP70 in the corneal epithelium was consistent with the in situ hybridization, being weak and mainly confined to the basal cells in control rats and increasing by 18 hours in the heat-treated rats. At 50 hours post-heat treatment, the immunostaining was denser than control in all corneal epithelial cells, especially in the apical portions of the basal and wing cells. These results suggest that SP70 may be an important factor in the response of the corneal epithelium to adverse environmental changes.

ISSN:0271-3683    

DOI:10.3109/02713689008999563

出版商:Taylor&Francis    

年代:1990

数据来源: Taylor

摘要:

To better define the growth requirements for corneal epithelial cells, methods for serumfree culture were established. Sheets of corneal epithelium from eyes of swine and rabbits were obtained by dispase treatment of corneal buttons, and single cells and small clumps were obtained by further dissociation with trypsin/EDTA. Growth of cells with epithelial-like morphology was readily achieved in low calcium MCDB 153 medium containing epidermal growth factor, insulin, hydrocortisone, and bovine pituitary extract. Primary cultures could be subcultivated at least four times at 1:6 split ratios. Examination of cultured corneal epithelial cells by transmission electron microscopy demonstrated a relatively undifferentiated phenotype. Elevation of calcium in the medium caused the reappearance of desmosomal junctions and a more typical corneal epithelial morphology.

ISSN:0271-3683    

DOI:10.3109/02713689008999564

出版商:Taylor&Francis    

年代:1990

数据来源: Taylor

摘要:

Synopsis of Ophthalmology, 6th Edition. Edited by J.J. Kanski. Wright (An Imprint of Butterworth Scientific), London, 1990.Ocumplastic&Orbital Emergencies, edited by J.V. Linberg, published by Appleton and Lange (a publishing division of Prentice Hall), East Norwalk, CT, 1990.Dictionary of Eye Terminology, 2nd Edition. Edited by Barbara Cassin and Sheila A.B. Solomon. Triad Publishing Company, Gainesville, FL, 1990.

ISSN:0271-3683    

DOI:10.3109/02713689008999565

出版商:Taylor&Francis    

年代:1990

数据来源: Taylor