摘要:

Previous work in our laboratory has shown thatCannabis sativa(marijuana) contains water soluble, high molecular weight components that have extremely potent intraocular pressure (IOP) lowering activity. Aqueous extraction of other plants has now shown that a number of them also contain components with potent IOP lowering activity in rabbits. These include tobacco, cabbage, lettuce, several greens,Senecio anonymus, Erigeron philadelphicus, and several others. Not all plants tested were active however, indicating that while more ubiquitous than originally thought, these materials are not apparently extractable from all plants. The chemical composition of the active fraction from tobacco was found to be different from that derived fromCannabis sativa.

ISSN:0271-3683    

DOI:10.3109/02713688709034837

出版商:Taylor&Francis    

年代:1987

数据来源: Taylor

摘要:

Several reports have appeared on the efficacy of topically applied 0.01% or 0.1% all-trans retinoic acid (0.04–0.4 millimolar) for treatment of xerophthalmia, conjunctival squamous metaplasia, and corneal epithelial erosions in humans and animals. An observation common to many of these studies is the occurrence of an adverse reaction to retinoic acid in the form of lid margin hyperemia and blepharoconjunctivitis. Since retinoic acid is biologically active at micro-molar to nanomolar concentrations, it may be possible to reduce side effects while maintaining therapeutic effectiveness by reducing the retinoic acid concentration in ophthalmic formulations. In the present study, topical 0.005% retinoic acid in petrolatum ointment reversed corneal keratinization in xerophthalmic, vitamin A-deficient rabbits in 3–4 days while 0.0005% (2 micromolar) retinoic acid ointment was effective in 4–6 days. Further clinical trials of topical retinoic acid for treatment of ocular surface disease should be conducted using micromolar concentrations of retinoic acid which are expected to maintain a therapeutic effect while reducing adverse reactions.

ISSN:0271-3683    

DOI:10.3109/02713688709034838

出版商:Taylor&Francis    

年代:1987

数据来源: Taylor

摘要:

In the presence of 0.1 M glycine, pH 2.5, a-crystallin dissociates into aggregates containing onlyαA chains plus monomericαB chains. Advantage has been taken of this to develop a method for the purification of theαA andαB chains by gel filtration on Sephadex G-75. This method gives high yields of purified subunits and avoids the risk of carbamylation associated with other methods which use high concentrations ofurea.

ISSN:0271-3683    

DOI:10.3109/02713688709034839

出版商:Taylor&Francis    

年代:1987

数据来源: Taylor