摘要:

The relationship between bulbar conjunctival hyperemia and temperature was investigated. Conjunctival redness was induced in eighteen volunteers by instilling hypertonic saline into the conjunctival sac. The degree of redness was estimated using a subjective grading scale. The subsequent changes in temperature of the nasal bulbar conjunctiva were monitored using an infra-red bolometer. Conjunctival hyperemia was significantly correlated with conjunctival temperature; the maximum response of a 3-grade change in redness was accompanied by an increase of 0.5°C in temperature. These findings confirm the classic association between inflammation, rubor and calor.

ISSN:0271-3683    

DOI:10.3109/02713688809031818

出版商:Taylor&Francis    

年代:1988

数据来源: Taylor

摘要:

Two methods were used to investigate cellular ion transport processes in confluent monolayers of cultured bovine corneal epithelial cells: measurements of membrane voltage (V) using conventional microelectrodes, and intracellular pH (pHi) measurements using the pH sensitive absorbance of intracellularly trapped 5(and 6)-carboxy-4′,5′dimethyl-fluorescein.(1) V averaged-39.2±0.9 mV (mean±SEM, n=71) with a range of-30 to-59 mV. Increasing extracellular potassium depolarized the cell membrane with a K+-slope of 43.3 mV/decade [K+] (for [K+] between 20 and 80 mM). Intracellular as well as extracellular acidification reversibly depolarized the cell membrane. Depolarization induced by 40 mM K+-pulses was smaller at extracellular pH (pHo) of 6.9 as compared to pHo= 7.9. These findings are compatible with a pH-sensitive K+conductance.(2) During steady state pHiwas 6.96±0.05 (mean±SEM, n=7). After intracellular acidification, induced by Nh4Cl-prepulse technique, pHiwas regulated back towards normal steady state pHi. Application of 1 mM amiloride reversibly inhibited pHirecovery. Furthermore, pHibackregulation was inhibited by removing sodium from the extracellular solution. The effect was reversible after readdition of sodium. These findings suggest that a Na+/H+exchange is present in corneal epithelial cells and participates in pHibackregulation after an intracellular acid load.

ISSN:0271-3683    

DOI:10.3109/02713688809031819

出版商:Taylor&Francis    

年代:1988

数据来源: Taylor

摘要:

The injection of a variety of antigens, including transplantation antigens, haptens, and viral glycoproteins, into the anterior chamber of eyes of mice produces a characteristic spectrum of immune responses in which suppressed cell mediated immunity dominates. This phenomenon is called Anterior Chamber Associated Immune Deviation (ACAID). Having recently demonstrated that soluble antigens also can induce ACAID in mice, we examined the possibility that an important soluble ocular antigen, retinal S antigen (S Ag) may be capable of inducing ACAID in rats. We have found that injection of S Ag with adjuvant into the anterior chamber of eyes of adult Lewis rats evokes neither a humoral nor a cell mediated immune response that can be detected. However, animals that received S Ag intracamerally in this manner did respond to a subsequent immunogenic dose of S Ag in CFA injected into the hind foot pads, by producing serum anti S Ag antibodies. Importantly, these rats failed to display antigen-specific delayed hypersensitivity. Moreover, lymphoid cells from anterior chamber pre-treated animals, when adoptively transferred, inhibited the development of S Ag-specific delayed hypersensitivity in naive recipients indicating that an active suppression mechanism had been generated. Thus, a soluble antigen injected intracamerally in adult Lewis rats can generate ACAID. The possibility that ACAID may be relevant to the development of S Ag induced uveitis is discussed.

ISSN:0271-3683    

DOI:10.3109/02713688809031820

出版商:Taylor&Francis    

年代:1988

数据来源: Taylor

摘要:

Colour Atlas of Ophthalmology, Second Edition. By A.S.M. Lim and I.J. Constable. Published by IOP Publishing Ltd. (Wright), Bristol, England, 1987.

ISSN:0271-3683    

DOI:10.3109/02713688809031821

出版商:Taylor&Francis    

年代:1988

数据来源: Taylor