摘要:

A 100 mm long and 5 mm wide filter paper strip enclosed in a transparent plastic sheath to prevent evaporation was used to determine the time dependence of wetting of the strip in the anesthetized eyes of dry eye patients and age-matched normals. Prior to the measurements, the paper strips were extracted by lipid solvents and marked at every millimeter length. Wetted length data were plotted against time and the resulting wetting curves were analyzed to obtain the time dependence of the tear secretion rate during lacrimation.Every eye studied exhibited an exponentially decaying tear secretion rate that could be characterized by three kinetic parameters per cycle: the initial and final tear secretion rates and the secretion decay coefficient. The eyes of the sicca patients showed a much simpler lacrimation pattern than did the controls: 60% of the dry eyes exhibited a one-cycle lacrimation pattern while only 5% of the normal group did so. The tear secretion kinetic parameters characterizing the lacrimation pattern reduced to one-cycle were compared. Both the dry eyes and the control eyes started to lacrimate at about the same high initial secretion rate. However, the lacrimation rate was found to decrease faster and to a lower final rate in dry eye patients as opposed to normal controls.

ISSN:0271-3683    

DOI:10.3109/02713688809031792

出版商:Taylor&Francis    

年代:1988

数据来源: Taylor

摘要:

Tear prealbumin previously isolated from the saline-extractable human ocular mucus was further examined for microheterogeneities using crossed-iirmunoelectrophoresis through development with antiserum to the isolate. The isolate was examined following the final two stages of its chromatographic purification by DEAE ion exchange and Sephadex G-75. The crossed-immunoelectrophoretic pattern of the tear prealbumin isolate, recovered following DEAE ion exchange chromatography, indicated the existence of two types of minor degrees of binding between tear prealbumin and serum albumin. One type appeared to be tightly associated while the other was interpreted as weakly bound, where serum albumin was separable from tear prealbumin in an electric field. The total association of serum albumin with tear prealbumin in this isolate was estimated to be about 3–7%. Crossed immunoelectrophoresis of the isolate after its final stage of purification on Sephadex G-75 chromatography showed the absence of the weakly bound serum albumin. Hawever, the tight tear prealbumin/serum albumin association remained, estimated to be 1–3%.

ISSN:0271-3683    

DOI:10.3109/02713688809031793

出版商:Taylor&Francis    

年代:1988

数据来源: Taylor

摘要:

Clinical Optics by Troy E. Fannin and Theodore Grosvenor. Published by Butterworths Scientific, Ltd.

ISSN:0271-3683    

DOI:10.3109/02713688809031794

出版商:Taylor&Francis    

年代:1988

数据来源: Taylor