摘要:

We quantitated the amount of collagen in corneas obtained from patients with keratoconus. Following a previously described procedure, we deparaffinized tissue sections and stained them with the collagen-specific dye Sirius red. Twenty-two (group I) of 31 keratoconus corneas examined were normal, whereas the remaining 9 (group II) showed reduced collagen staining per mm2of surface area compared with the controls. These results agreed with our previous data demonstrating heterogeneity in keratoconus in terms of collagen content analyzed biochemically in addition, we adapted another histochemical method, using safranin O, to measure the polyanion (mainly glycosaminoglycan) content in paraffin sections of the same specimens. All except two keratoconus corneas showed markedly higher polyanion staining per mm2of surface area than the controls. This suggests that keratoconus corneas may contain an increased amount of glycosaminoglycans and other polyanions. Such an abnormality may be related to some of the pathologic changes seen in these corneas.

ISSN:0271-3683    

DOI:10.3109/02713688809047024

出版商:Taylor&Francis    

年代:1988

数据来源: Taylor

摘要:

Experimental autoimmune uveitis (EAU) was observed in Hartley guinea pigs following Immunization with two small synthetic peptides, peptide M and peptide M15L, which correspond to the amino acid sequence of a well-characterized region of bovine S-antigen. Groups of guinea pigs were immunized with 100γg of each peptide in complete Freund's adjuvant and examined at regular intervals for the development of disease. Approximately two weeks later, an EAU was present which was characterized clinically by iris and pericorneal hyperemia. Histopathologically, a severe inflammatory response involving the uveal tract and retina was observed. In these eyes the photoreceptor cell layer of the retina was destroyed. A subretinal exudate containing mononuclear cells and polymorphonuclear leukocytes was also present. In addition, animals with EAU showed an associated pinealitis characterized by a lymphocytic infiltration of the subcapsular and central area of the pineal gland. Furthermore, draining lymph node cells of guinea pigs immunized with peptide M showed strong in vitro proliferative responses towards peptide M as measured by3H thymidine uptake. These results demonstrate the existence of at least one common pathogenic epitope in bovine S-antigen for the induction of EAU in Hartley guinea pigs and Lewis rats.

ISSN:0271-3683    

DOI:10.3109/02713688809047025

出版商:Taylor&Francis    

年代:1988

数据来源: Taylor

ISSN:0271-3683    

DOI:10.3109/02713688809047026

出版商:Taylor&Francis    

年代:1988

数据来源: Taylor