摘要:

Monkeys immunized with bovine IRBP-derived synthetic peptides R4 (sequence 1158–1180) or R14 (1169–1191) developed EAU which was detected by both clinical and histological examinations. The inflammation localized mainly in the choroid, with only minor changes being noticed in the adjacent retinal tissue. EAU developed in only one of the two monkeys immunized with each of the peptides and the animals with disease also showed higher levels of cellular immunity toward the immunizing peptide than did the monkeys with no disease. The cellular immune responses, measured by the lymphocyte proliferation assay, were specific toward the immunizing peptides, with no cross responsiveness to whole IRBP. This finding suggests that the two uveitogenic peptides were non-immunodominant in the tested monkeys. In contrast, peptide R14 is highly immunodominant in the Lewis rat. Also, the fine specificity of the monkey response to R14 differed from that of the Lewis rat. The possible genetic control of the monkey susceptibility to EAU induction by the peptides is discussed and the unique finding of an autoimmune disease induction by a non-immunodominant peptide is underscored.

ISSN:0271-3683    

DOI:10.3109/02713689008995206

出版商:Taylor&Francis    

年代:1990

数据来源: Taylor

摘要:

A method capable of the determination of pmole quantities of myo-inositol in mgr amounts of tissue by anion exchange chromatography is detailed for use in lens and potentially other tissues. Samples were rendered protein-free through ZnS04/Ba(0H)2precipitation, lyophilized and reconstituted in water just prior to analysis. An aliquot of sample was injected onto an anion exchange column and eluted with a 0.045 M sodium hydroxide mobile phase. Each analysis requires 30 minutes to complete. Average recovery of myo-inositol added to lens sample prior to injection was 100.6%. The coefficient of variation for repeated sample injections was 2.9%. Rabbit lens averaged 11.4μmol/gr wet weight with epithelium containing 8.5μmol/gr wet weight while human lens contained 20.1μmol/gr wet weight and human epithelial cells had 17.5 nmol/mgr protein.

ISSN:0271-3683    

DOI:10.3109/02713689008995207

出版商:Taylor&Francis    

年代:1990

数据来源: Taylor