摘要:

The results of this investigation demonstrate that the IgA antibody in tears inhibits the attachment of herpes simplex virus (HSV) to cells and implicate immunoglobulin A (IgA) isotype antibody in the protection of the ocular surface. Tears were collected from the eyes of infected and uninfected rabbits and tested for the presence of infectious virus, for viral neutralization titer, and in an enzyme-linked immunoassay (ELISA) for antibody to HSV. Neither the tears from the uninfected rabbits nor the tears from the infected rabbits produced viral cytopathic effects on indicator cells in vitro. Tears from uninfected rabbits had no virus neutralization capacity while the tears from infected rabbits had titers of 1:10 to 1:40. Tears collected from HSV-1 infected rabbits, but not uninfected rabbits, had anti-HSV antibody titers as determined by the enzyme-linked immunosorbent assay. Lectin affinity column enrichment of IgA from the tears of uninfected and infected rabbits was examined for the capacity to block viral adsorption to cells using32P-labeled virus. It was found that exposure of the radio-actively-labeled virus to the purified IgA fraction of tears from infected rabbits reduced the amount of virus binding to Vero cells, whereas the purified IgA obtained from uninfected tears had minimal activity. These results may imply a role for the IgA-containing fraction of tears from HSV-infected animals in preventing viral attachment to cells and suggest that antibody of this isotype plays a protective role at the ocular surface.

ISSN:0271-3683    

DOI:10.3109/02713688809047037

出版商:Taylor&Francis    

年代:1988

数据来源: Taylor

摘要:

Rabbits were treated with the cyclooxygenase inhibitors indomethacin (2.5 and 10 mg/kg; IP) or flunixin meglumine (2.2 and 8.8 mg/kg; IM), or the dual cyclooxygenase/lipoxygenase inhibitor BW755C (10 and 30 mg/kg; IP) (administered 4 times over 25 hours). One hour following drug administration, 10 ng of E. coli endotoxin was injected into the vitreal chamber and the subsequent inflammatory response was measured 24 h later. Indomethacin was the most effective inhibitor of prostaglandin (PG) E2and PGF2a accumulation in the aqueous humor and their ex vivo production by the lens. It was the only agent that decreased iridal hyperemia and protein concentration in the aqueous humor. Flunixin decreased PG production, although generally to a lesser degree than indomethacin. BW755C produced modest inhibition of PG production in the aqueous humor, but did not affect PG production by the lens. The high dose of BW755C also reduced 5-hydroxyeicosatetraenoic acid (5-HETE) levels in the aqueous humor, but did not alter production by the lens. The results indicate that despite drastic reductions in PG levels in the aqueous humor and PG production by the lens, only modest decreases in aqueous humor protein concentration and iridal hyperemia were achieved. Furthermore, 5-HETE does not appear to be important in the modulation of these inflammatory signs, although it is possible that larger decreases in its production could result in more dramatic effects.

ISSN:0271-3683    

DOI:10.3109/02713688809047038

出版商:Taylor&Francis    

年代:1988

数据来源: Taylor

摘要:

Systemic Inflammatory Disease and the Eyeby W.J. Dinning. Published by Butterworth Scientific Ltd.Uveitis. A Colour Manual of Diagnosis and Treatmentby Jack J. Kanski. Published by Butterworth Scientific Ltd.

ISSN:0271-3683    

DOI:10.3109/02713688809047039

出版商:Taylor&Francis    

年代:1988

数据来源: Taylor